Investigating the role for adaptation of the microbial community to transform trace organic chemicals during managed aquifer recharge

Handle URI:
http://hdl.handle.net/10754/563564
Title:
Investigating the role for adaptation of the microbial community to transform trace organic chemicals during managed aquifer recharge
Authors:
Alidina, Mazahirali; Li, Dong; Drewes, Jorg
Abstract:
This study was undertaken to investigate whether adaptation by pre-exposure to trace organic chemicals (TOrCs) was necessary for microbial transformation during managed aquifer recharge (MAR). Two pairs of laboratory-scale soil columns, each receiving a different primary substrate, were utilized to simulate the dominant bulk organic carbon present in MAR systems receiving wastewater effluent of varying quality and having undergone different degrees of pre-treatment, as well as organic carbon prevalent at different stages of subsurface travel. Each pair of columns consisted of duplicate set-ups receiving the same feed solution with only one pre-exposed to a suite of eight TOrCs for approximately ten months. Following the pre-exposure period, a spiking experiment was conducted in which the non-exposed columns also received the same suite of TOrCs. TOrC attenuation was quantified for the pre- and non-exposed columns of each pair during the spiking experiment. The microbial community structure and function of these systems were characterized by pyrosequencing of 16S rRNA gene and metagenomics, respectively. Biotransformation rather than sorption was identified as the dominant removal mechanism for almost all the TOrCs (except triclocarban). Similar removal efficiencies were observed between pre-exposed and non-exposed columns for most TOrCs. No obvious differences in microbial community structure were revealed between pre- and non-exposed columns. Using metagenomics, biotransformation capacity potentials of the microbial community present were also similar between pre- and non-exposed columns of each pair. Overall, the pre-exposure of MAR systems to TOrCs at ng/L levels did not affect their attenuation and had no obvious influence on the resulting microbial community structure and function. Thus, other factors such as bioavailability of the primary substrate play a greater role regarding biotransformation of TOrCs. These results indicate that MAR systems adapted to a primary substrate are capable of degrading TOrC without necessarily being pre-exposed to them, making MAR a robust treatment barrier for biodegradable TOrCs. © 2014 Elsevier Ltd.
KAUST Department:
Water Desalination and Reuse Research Center (WDRC); Water Desalination & Reuse Research Cntr; Environmental Science and Engineering Program
Publisher:
Elsevier BV
Journal:
Water Research
Issue Date:
Jun-2014
DOI:
10.1016/j.watres.2014.02.046
PubMed ID:
24681234
Type:
Article
ISSN:
00431354
Sponsors:
This research was supported by discretionary investigator funds at King Abdullah University of Science and Technology (KAUST). The material presented is also based in part upon work supported by the National Science Foundation under Cooperative Agreement EEC-1028968. The authors are thankful for technical assistance provided by Prof. Pascal Saikaly at KAUST as well as Prof. Jonathan O. Sharp at Colorado School of Mines.
Appears in Collections:
Articles; Environmental Science and Engineering Program; Water Desalination and Reuse Research Center (WDRC)

Full metadata record

DC FieldValue Language
dc.contributor.authorAlidina, Mazahiralien
dc.contributor.authorLi, Dongen
dc.contributor.authorDrewes, Jorgen
dc.date.accessioned2015-08-03T11:54:35Zen
dc.date.available2015-08-03T11:54:35Zen
dc.date.issued2014-06en
dc.identifier.issn00431354en
dc.identifier.pmid24681234en
dc.identifier.doi10.1016/j.watres.2014.02.046en
dc.identifier.urihttp://hdl.handle.net/10754/563564en
dc.description.abstractThis study was undertaken to investigate whether adaptation by pre-exposure to trace organic chemicals (TOrCs) was necessary for microbial transformation during managed aquifer recharge (MAR). Two pairs of laboratory-scale soil columns, each receiving a different primary substrate, were utilized to simulate the dominant bulk organic carbon present in MAR systems receiving wastewater effluent of varying quality and having undergone different degrees of pre-treatment, as well as organic carbon prevalent at different stages of subsurface travel. Each pair of columns consisted of duplicate set-ups receiving the same feed solution with only one pre-exposed to a suite of eight TOrCs for approximately ten months. Following the pre-exposure period, a spiking experiment was conducted in which the non-exposed columns also received the same suite of TOrCs. TOrC attenuation was quantified for the pre- and non-exposed columns of each pair during the spiking experiment. The microbial community structure and function of these systems were characterized by pyrosequencing of 16S rRNA gene and metagenomics, respectively. Biotransformation rather than sorption was identified as the dominant removal mechanism for almost all the TOrCs (except triclocarban). Similar removal efficiencies were observed between pre-exposed and non-exposed columns for most TOrCs. No obvious differences in microbial community structure were revealed between pre- and non-exposed columns. Using metagenomics, biotransformation capacity potentials of the microbial community present were also similar between pre- and non-exposed columns of each pair. Overall, the pre-exposure of MAR systems to TOrCs at ng/L levels did not affect their attenuation and had no obvious influence on the resulting microbial community structure and function. Thus, other factors such as bioavailability of the primary substrate play a greater role regarding biotransformation of TOrCs. These results indicate that MAR systems adapted to a primary substrate are capable of degrading TOrC without necessarily being pre-exposed to them, making MAR a robust treatment barrier for biodegradable TOrCs. © 2014 Elsevier Ltd.en
dc.description.sponsorshipThis research was supported by discretionary investigator funds at King Abdullah University of Science and Technology (KAUST). The material presented is also based in part upon work supported by the National Science Foundation under Cooperative Agreement EEC-1028968. The authors are thankful for technical assistance provided by Prof. Pascal Saikaly at KAUST as well as Prof. Jonathan O. Sharp at Colorado School of Mines.en
dc.publisherElsevier BVen
dc.subjectAdaptationen
dc.subjectBiotransformationen
dc.subjectCo-metabolismen
dc.subjectManaged aquifer rechargeen
dc.subjectTrace organic chemicalsen
dc.titleInvestigating the role for adaptation of the microbial community to transform trace organic chemicals during managed aquifer rechargeen
dc.typeArticleen
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)en
dc.contributor.departmentWater Desalination & Reuse Research Cntren
dc.contributor.departmentEnvironmental Science and Engineering Programen
dc.identifier.journalWater Researchen
dc.contributor.institutionNSF Engineering Research Center ReNUWIt, Civil and Environmental Engineering, Colorado School of Mines, Golden, CO, United Statesen
dc.contributor.institutionUrban Water Systems Engineering, Technische Universität München, Garching, Germanyen
kaust.authorAlidina, Mazahiralien
kaust.authorLi, Dongen
kaust.authorDrewes, Jorgen
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