Validation of microsatellite multiplexes for parentage analysis in a coral reef fish (Lutjanus carponotatus, Lutjanidae)

Handle URI:
http://hdl.handle.net/10754/563557
Title:
Validation of microsatellite multiplexes for parentage analysis in a coral reef fish (Lutjanus carponotatus, Lutjanidae)
Authors:
Harrison, Hugo B.; Feldheim, Kevin Andrew; Jones, Geoffrey P.; Mansour, Hicham; Perumal, Sadhasivam; Williamson, David H.; Berumen, Michael L. ( 0000-0003-2463-2742 )
Abstract:
Parentage analysis is an important tool for identifying connectivity patterns in coral reef fishes, but often requires numerous highly polymorphic markers. We isolated 21 polymorphic microsatellite markers from the stripey snapper, Lutjanus carponotatus and describe their integration into three multiplex PCRs. All markers were highly polymorphic with a mean of 24.9 ± 1.8 SE alleles per locus and an average observed heterozygosity of 0.797 ± 0.038 SE across 285 genotyped individuals. Using a simulated dataset, we conclude that the complete marker set provides sufficient resolution to resolve parent–offspring relationships in natural populations with 99.6 ± 0.1 % accuracy in parentage assignments. This multiplex assay provides an effective means of investigating larval dispersal and population connectivity in this fishery-targeted coral reef fish species and informing the design of marine protected area networks for biodiversity conservation and fisheries management.
KAUST Department:
Biosciences Core Lab; Red Sea Research Center (RSRC); Biological and Environmental Sciences and Engineering (BESE) Division; Marine Science Program; Core Labs; Reef Ecology Lab
Publisher:
Springer Nature
Journal:
Conservation Genetics Resources
Issue Date:
25-May-2014
DOI:
10.1007/s12686-014-0226-4
Type:
Article
ISSN:
18777252
Sponsors:
We are grateful to volunteers who assisted with sample collections. This project was supported by KAUST awards CRG-1-2012-BER-002 and OCRF-SPCF-2011-BER-001, as well as the Australian government's National Environmental Research Program (NERP), Tropical Ecosystem's Hub. It formed part of Project 8.3: The significance of no-take marine protected areas to regional recruitment and population persistence on the GBR. Additional support was provided by the ARC Centre of Excellence for Coral Reef Studies and the KAUST Red Sea Research Center.
Appears in Collections:
Articles; Red Sea Research Center (RSRC); Marine Science Program; Bioscience Core Lab; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorHarrison, Hugo B.en
dc.contributor.authorFeldheim, Kevin Andrewen
dc.contributor.authorJones, Geoffrey P.en
dc.contributor.authorMansour, Hichamen
dc.contributor.authorPerumal, Sadhasivamen
dc.contributor.authorWilliamson, David H.en
dc.contributor.authorBerumen, Michael L.en
dc.date.accessioned2015-08-03T11:54:25Zen
dc.date.available2015-08-03T11:54:25Zen
dc.date.issued2014-05-25en
dc.identifier.issn18777252en
dc.identifier.doi10.1007/s12686-014-0226-4en
dc.identifier.urihttp://hdl.handle.net/10754/563557en
dc.description.abstractParentage analysis is an important tool for identifying connectivity patterns in coral reef fishes, but often requires numerous highly polymorphic markers. We isolated 21 polymorphic microsatellite markers from the stripey snapper, Lutjanus carponotatus and describe their integration into three multiplex PCRs. All markers were highly polymorphic with a mean of 24.9 ± 1.8 SE alleles per locus and an average observed heterozygosity of 0.797 ± 0.038 SE across 285 genotyped individuals. Using a simulated dataset, we conclude that the complete marker set provides sufficient resolution to resolve parent–offspring relationships in natural populations with 99.6 ± 0.1 % accuracy in parentage assignments. This multiplex assay provides an effective means of investigating larval dispersal and population connectivity in this fishery-targeted coral reef fish species and informing the design of marine protected area networks for biodiversity conservation and fisheries management.en
dc.description.sponsorshipWe are grateful to volunteers who assisted with sample collections. This project was supported by KAUST awards CRG-1-2012-BER-002 and OCRF-SPCF-2011-BER-001, as well as the Australian government's National Environmental Research Program (NERP), Tropical Ecosystem's Hub. It formed part of Project 8.3: The significance of no-take marine protected areas to regional recruitment and population persistence on the GBR. Additional support was provided by the ARC Centre of Excellence for Coral Reef Studies and the KAUST Red Sea Research Center.en
dc.publisherSpringer Natureen
dc.subjectCoral reef fishen
dc.subjectDispersalen
dc.subjectLutjanus carponotatusen
dc.subjectMicrosatellite multiplexen
dc.subjectParentage analysisen
dc.titleValidation of microsatellite multiplexes for parentage analysis in a coral reef fish (Lutjanus carponotatus, Lutjanidae)en
dc.typeArticleen
dc.contributor.departmentBiosciences Core Laben
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentMarine Science Programen
dc.contributor.departmentCore Labsen
dc.contributor.departmentReef Ecology Laben
dc.identifier.journalConservation Genetics Resourcesen
dc.contributor.institutionCentre of Excellence for Coral Reef Studies, James Cook UniversityTownsville, QLD, Australiaen
dc.contributor.institutionPritzker Laboratory for Molecular Systematics and Evolution, The Field Museum, 1400 S. Lake Shore DriveChicago, IL, United Statesen
dc.contributor.institutionSchool of Marine and Tropical Biology, James Cook UniversityTownsville, QLD, Australiaen
kaust.authorMansour, Hichamen
kaust.authorPerumal, Sadhasivamen
kaust.authorBerumen, Michael L.en
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