"light-on" sensing of antioxidants using gold nanoclusters

Handle URI:
http://hdl.handle.net/10754/563552
Title:
"light-on" sensing of antioxidants using gold nanoclusters
Authors:
Hu, Lianzhe; Deng, Lin ( 0000-0001-8954-5610 ) ; Alsaiari, Shahad K.; Zhang, Dingyuan; Khashab, Niveen M. ( 0000-0003-2728-0666 )
Abstract:
Depletion of intracellular antioxidants is linked to major cytotoxic events and cellular disorders, such as oxidative stress and multiple sclerosis. In addition to medical diagnosis, determining the concentration of antioxidants in foodstuffs, food preservatives, and cosmetics has proved to be very vital. Gold nanoclusters (Au-NCs) have a core size below 2 nm and contain several metal atoms. They have interesting photophysical properties, are readily functionalized, and are safe to use in various biomedical applications. Herein, a simple and quantitative spectroscopic method based on Au-NCs is developed to detect and image antioxidants such as ascorbic acid. The sensing mechanism is based on the fact that antioxidants can protect the fluorescence of Au-NCs against quenching by highly reactive oxygen species. Our method shows great accuracy when employed to detect the total antioxidant capacity in commercial fruit juice. Moreover, confocal fluorescence microscopy images of HeLa cells show that this approach can be successfully used to image antioxidant levels in living cells. Finally, the potential application of this "light-on" detection method in multiple logic gate fabrication was discussed using the fluorescence intensity of Au-NCs as output. © 2014 American Chemical Society.
KAUST Department:
Advanced Membranes and Porous Materials Research Center; Smart Hybrid Materials (SHMs) lab; Physical Sciences and Engineering (PSE) Division; Chemical Science Program; Bioscience Program
Publisher:
American Chemical Society (ACS)
Journal:
Analytical Chemistry
Issue Date:
20-May-2014
DOI:
10.1021/ac500528m
Type:
Article
ISSN:
00032700
Sponsors:
The authors gratefully acknowledge King Abdullah University of Science and Technology (KAUST) for the support of this work.
Appears in Collections:
Articles; Bioscience Program; Advanced Membranes and Porous Materials Research Center; Physical Sciences and Engineering (PSE) Division; Controlled Release and Delivery Laboratory; Chemical Science Program

Full metadata record

DC FieldValue Language
dc.contributor.authorHu, Lianzheen
dc.contributor.authorDeng, Linen
dc.contributor.authorAlsaiari, Shahad K.en
dc.contributor.authorZhang, Dingyuanen
dc.contributor.authorKhashab, Niveen M.en
dc.date.accessioned2015-08-03T11:54:18Zen
dc.date.available2015-08-03T11:54:18Zen
dc.date.issued2014-05-20en
dc.identifier.issn00032700en
dc.identifier.doi10.1021/ac500528men
dc.identifier.urihttp://hdl.handle.net/10754/563552en
dc.description.abstractDepletion of intracellular antioxidants is linked to major cytotoxic events and cellular disorders, such as oxidative stress and multiple sclerosis. In addition to medical diagnosis, determining the concentration of antioxidants in foodstuffs, food preservatives, and cosmetics has proved to be very vital. Gold nanoclusters (Au-NCs) have a core size below 2 nm and contain several metal atoms. They have interesting photophysical properties, are readily functionalized, and are safe to use in various biomedical applications. Herein, a simple and quantitative spectroscopic method based on Au-NCs is developed to detect and image antioxidants such as ascorbic acid. The sensing mechanism is based on the fact that antioxidants can protect the fluorescence of Au-NCs against quenching by highly reactive oxygen species. Our method shows great accuracy when employed to detect the total antioxidant capacity in commercial fruit juice. Moreover, confocal fluorescence microscopy images of HeLa cells show that this approach can be successfully used to image antioxidant levels in living cells. Finally, the potential application of this "light-on" detection method in multiple logic gate fabrication was discussed using the fluorescence intensity of Au-NCs as output. © 2014 American Chemical Society.en
dc.description.sponsorshipThe authors gratefully acknowledge King Abdullah University of Science and Technology (KAUST) for the support of this work.en
dc.publisherAmerican Chemical Society (ACS)en
dc.title"light-on" sensing of antioxidants using gold nanoclustersen
dc.typeArticleen
dc.contributor.departmentAdvanced Membranes and Porous Materials Research Centeren
dc.contributor.departmentSmart Hybrid Materials (SHMs) laben
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.contributor.departmentChemical Science Programen
dc.contributor.departmentBioscience Programen
dc.identifier.journalAnalytical Chemistryen
kaust.authorHu, Lianzheen
kaust.authorDeng, Linen
kaust.authorAlsaiari, Shahad K.en
kaust.authorZhang, Dingyuanen
kaust.authorKhashab, Niveen M.en
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