The putative E3 ubiquitin ligase ECERIFERUM9 regulates abscisic acid biosynthesis and response during seed germination and postgermination growth in arabidopsis

Handle URI:
http://hdl.handle.net/10754/563542
Title:
The putative E3 ubiquitin ligase ECERIFERUM9 regulates abscisic acid biosynthesis and response during seed germination and postgermination growth in arabidopsis
Authors:
Zhao, Huayan; Zhang, Huoming ( 0000-0001-5416-0358 ) ; Cui, Peng ( 0000-0003-3076-0070 ) ; Ding, Feng ( 0000-0001-8237-4062 ) ; Wang, Guangchao; Li, Rongjun; Jenks, Matthew A.; Lü, Shiyou; Xiong, Liming ( 0000-0001-8099-0806 )
Abstract:
The ECERIFERUM9 (CER9) gene encodes a putative E3 ubiquitin ligase that functions in cuticle biosynthesis and the maintenance of plant water status. Here, we found that CER9 is also involved in abscisic acid (ABA) signaling in seeds and young seedlings of Arabidopsis (Arabidopsis thaliana). The germinated embryos of the mutants exhibited enhanced sensitivity to ABA during the transition from reversible dormancy to determinate seedling growth. Expression of the CER9 gene is closely related to ABA levels and displays a similar pattern to that of ABSCISIC ACID-INSENSITIVE5 (ABI5), which encodes a positive regulator of ABA responses in seeds. cer9 mutant seeds exhibited delayed germination that is independent of seed coat permeability. Quantitative proteomic analyses showed that cer9 seeds had a protein profile similar to that of the wild type treated with ABA. Transcriptomics analyses revealed that genes involved in ABA biosynthesis or signaling pathways were differentially regulated in cer9 seeds. Consistent with this, high levels of ABA were detected in dry seeds of cer9. Blocking ABA biosynthesis by fluridone treatment or by combining an ABA-deficient mutation with cer9 attenuated the phenotypes of cer9. Whereas introduction of the abi1-1, abi3-1, or abi4-103 mutation could completely eliminate the ABA hypersensitivity of cer9, introduction of abi5 resulted only in partial suppression. These results indicate that CER9 is a novel negative regulator of ABA biosynthesis and the ABA signaling pathway during seed germination. © 2014 American Society of Plant Biologists. All Rights Reserved.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Biosciences Core Lab; Bioscience Program; Core Labs; Plant Stress Genomics Research Lab
Publisher:
American Society of Plant Biologists (ASPB)
Journal:
PLANT PHYSIOLOGY
Issue Date:
8-May-2014
DOI:
10.1104/pp.114.239699
PubMed ID:
24812105
PubMed Central ID:
PMC4081335
Type:
Article
ISSN:
00320889
Sponsors:
This work was supported by King Abdullah University of Science and Technology (to L.X.) and by the Natural Science Foundation of China (grant no. 113137033816 to S.L.).
Additional Links:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4081335
Appears in Collections:
Articles; Bioscience Program; Bioscience Core Lab; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorZhao, Huayanen
dc.contributor.authorZhang, Huomingen
dc.contributor.authorCui, Pengen
dc.contributor.authorDing, Fengen
dc.contributor.authorWang, Guangchaoen
dc.contributor.authorLi, Rongjunen
dc.contributor.authorJenks, Matthew A.en
dc.contributor.authorLü, Shiyouen
dc.contributor.authorXiong, Limingen
dc.date.accessioned2015-08-03T11:54:03Zen
dc.date.available2015-08-03T11:54:03Zen
dc.date.issued2014-05-08en
dc.identifier.issn00320889en
dc.identifier.pmid24812105en
dc.identifier.doi10.1104/pp.114.239699en
dc.identifier.urihttp://hdl.handle.net/10754/563542en
dc.description.abstractThe ECERIFERUM9 (CER9) gene encodes a putative E3 ubiquitin ligase that functions in cuticle biosynthesis and the maintenance of plant water status. Here, we found that CER9 is also involved in abscisic acid (ABA) signaling in seeds and young seedlings of Arabidopsis (Arabidopsis thaliana). The germinated embryos of the mutants exhibited enhanced sensitivity to ABA during the transition from reversible dormancy to determinate seedling growth. Expression of the CER9 gene is closely related to ABA levels and displays a similar pattern to that of ABSCISIC ACID-INSENSITIVE5 (ABI5), which encodes a positive regulator of ABA responses in seeds. cer9 mutant seeds exhibited delayed germination that is independent of seed coat permeability. Quantitative proteomic analyses showed that cer9 seeds had a protein profile similar to that of the wild type treated with ABA. Transcriptomics analyses revealed that genes involved in ABA biosynthesis or signaling pathways were differentially regulated in cer9 seeds. Consistent with this, high levels of ABA were detected in dry seeds of cer9. Blocking ABA biosynthesis by fluridone treatment or by combining an ABA-deficient mutation with cer9 attenuated the phenotypes of cer9. Whereas introduction of the abi1-1, abi3-1, or abi4-103 mutation could completely eliminate the ABA hypersensitivity of cer9, introduction of abi5 resulted only in partial suppression. These results indicate that CER9 is a novel negative regulator of ABA biosynthesis and the ABA signaling pathway during seed germination. © 2014 American Society of Plant Biologists. All Rights Reserved.en
dc.description.sponsorshipThis work was supported by King Abdullah University of Science and Technology (to L.X.) and by the Natural Science Foundation of China (grant no. 113137033816 to S.L.).en
dc.publisherAmerican Society of Plant Biologists (ASPB)en
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4081335en
dc.titleThe putative E3 ubiquitin ligase ECERIFERUM9 regulates abscisic acid biosynthesis and response during seed germination and postgermination growth in arabidopsisen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentBiosciences Core Laben
dc.contributor.departmentBioscience Programen
dc.contributor.departmentCore Labsen
dc.contributor.departmentPlant Stress Genomics Research Laben
dc.identifier.journalPLANT PHYSIOLOGYen
dc.identifier.pmcidPMC4081335en
dc.contributor.institutionKey Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, Chinaen
dc.contributor.institutionDivision of Plant and Soil Sciences, West Virginia University, Morgantown, WV 26506-6108, United Statesen
kaust.authorZhao, Huayanen
kaust.authorZhang, Huomingen
kaust.authorCui, Pengen
kaust.authorDing, Fengen
kaust.authorWang, Guangchaoen
kaust.authorXiong, Limingen
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