The polyadenylation factor subunit CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR30: A key factor of programmed cell death and a regulator of immunity in arabidopsis

Handle URI:
http://hdl.handle.net/10754/563489
Title:
The polyadenylation factor subunit CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR30: A key factor of programmed cell death and a regulator of immunity in arabidopsis
Authors:
Bruggeman, Quentin; Garmier, Marie; De Bont, Linda; Soubigou-Taconnat, Ludivine; Mazubert, Christelle; Benhamed, Moussa ( 0000-0002-4181-1702 ) ; Raynaud, Cécile; Bergounioux, Catherine; Delarue, Marianne
Abstract:
Programmed cell death (PCD) is essential for several aspects of plant life, including development and stress responses. Indeed, incompatible plant-pathogen interactions are well known to induce the hypersensitive response, a localized cell death. Mutational analyses have identified several key PCD components, and we recently identified the mips1 mutant of Arabidopsis (Arabidopsis thaliana), which is deficient for the key enzyme catalyzing the limiting step of myoinositol synthesis. One of the most striking features of mips1 is the light-dependent formation of lesions on leaves due to salicylic acid (SA)-dependent PCD, revealing roles for myoinositol or inositol derivatives in the regulation of PCD. Here, we identified a regulator of plant PCD by screening for mutants that display transcriptomic profiles opposing that of the mips1 mutant. Our screen identified the oxt6 mutant, which has been described previously as being tolerant to oxidative stress. In the oxt6 mutant, a transfer DNA is inserted in the CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR30 (CPSF30) gene, which encodes a polyadenylation factor subunit homolog. We show that CPSF30 is required for lesion formation in mips1 via SA-dependent signaling, that the prodeath function of CPSF30 is not mediated by changes in the glutathione status, and that CPSF30 activity is required for Pseudomonas syringae resistance. We also show that the oxt6 mutation suppresses cell death in other lesion-mimic mutants, including lesion-simulating disease1, mitogen-activated protein kinase4, constitutive expressor of pathogenesis-related genes5, and catalase2, suggesting that CPSF30 and, thus, the control of messenger RNA 3′ end processing, through the regulation of SA production, is a key component of plant immune responses. © 2014 American Society of Plant Biologists. All rights reserved.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Center for Desert Agriculture; Bioscience Program; Chromatin and development Research Group
Publisher:
American Society of Plant Biologists (ASPB)
Journal:
PLANT PHYSIOLOGY
Issue Date:
4-Apr-2014
DOI:
10.1104/pp.114.236083
PubMed ID:
24706550
PubMed Central ID:
PMC4044851
Type:
Article
ISSN:
00320889
Sponsors:
This work was supported by the Centre National de la Recherche Scientifique, the Universite Paris-Sud, the Agence Nationale de la Recherche (grant no. MAPK-IPS ANR-2010-BLAN-1613-02), and the Labex Saclay Plant Sciences.
Additional Links:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4044851
Appears in Collections:
Articles; Bioscience Program; Desert Agriculture Initiative; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorBruggeman, Quentinen
dc.contributor.authorGarmier, Marieen
dc.contributor.authorDe Bont, Lindaen
dc.contributor.authorSoubigou-Taconnat, Ludivineen
dc.contributor.authorMazubert, Christelleen
dc.contributor.authorBenhamed, Moussaen
dc.contributor.authorRaynaud, Cécileen
dc.contributor.authorBergounioux, Catherineen
dc.contributor.authorDelarue, Marianneen
dc.date.accessioned2015-08-03T11:52:43Zen
dc.date.available2015-08-03T11:52:43Zen
dc.date.issued2014-04-04en
dc.identifier.issn00320889en
dc.identifier.pmid24706550en
dc.identifier.doi10.1104/pp.114.236083en
dc.identifier.urihttp://hdl.handle.net/10754/563489en
dc.description.abstractProgrammed cell death (PCD) is essential for several aspects of plant life, including development and stress responses. Indeed, incompatible plant-pathogen interactions are well known to induce the hypersensitive response, a localized cell death. Mutational analyses have identified several key PCD components, and we recently identified the mips1 mutant of Arabidopsis (Arabidopsis thaliana), which is deficient for the key enzyme catalyzing the limiting step of myoinositol synthesis. One of the most striking features of mips1 is the light-dependent formation of lesions on leaves due to salicylic acid (SA)-dependent PCD, revealing roles for myoinositol or inositol derivatives in the regulation of PCD. Here, we identified a regulator of plant PCD by screening for mutants that display transcriptomic profiles opposing that of the mips1 mutant. Our screen identified the oxt6 mutant, which has been described previously as being tolerant to oxidative stress. In the oxt6 mutant, a transfer DNA is inserted in the CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR30 (CPSF30) gene, which encodes a polyadenylation factor subunit homolog. We show that CPSF30 is required for lesion formation in mips1 via SA-dependent signaling, that the prodeath function of CPSF30 is not mediated by changes in the glutathione status, and that CPSF30 activity is required for Pseudomonas syringae resistance. We also show that the oxt6 mutation suppresses cell death in other lesion-mimic mutants, including lesion-simulating disease1, mitogen-activated protein kinase4, constitutive expressor of pathogenesis-related genes5, and catalase2, suggesting that CPSF30 and, thus, the control of messenger RNA 3′ end processing, through the regulation of SA production, is a key component of plant immune responses. © 2014 American Society of Plant Biologists. All rights reserved.en
dc.description.sponsorshipThis work was supported by the Centre National de la Recherche Scientifique, the Universite Paris-Sud, the Agence Nationale de la Recherche (grant no. MAPK-IPS ANR-2010-BLAN-1613-02), and the Labex Saclay Plant Sciences.en
dc.publisherAmerican Society of Plant Biologists (ASPB)en
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4044851en
dc.titleThe polyadenylation factor subunit CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR30: A key factor of programmed cell death and a regulator of immunity in arabidopsisen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentCenter for Desert Agricultureen
dc.contributor.departmentBioscience Programen
dc.contributor.departmentChromatin and development Research Groupen
dc.identifier.journalPLANT PHYSIOLOGYen
dc.identifier.pmcidPMC4044851en
dc.contributor.institutionUniversité Paris-Sud, Institut de Biologie des Plantes, Unité Mixte de Recherche Centre National de la Recherche Scientifique 8618, Saclay Plant Sciences, F-91405 Orsay, Franceen
dc.contributor.institutionUnité de Recherche en Génomique Végétale, Unité Mixte de Recherche-Institut National de la Recherche Agronomique 1165, Centre National de la Recherche Scientifique 8114, 91 057 Evry cedex, Franceen
kaust.authorBenhamed, Moussaen

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