Magneto-mechanical trapping systems for biological target detection

Handle URI:
http://hdl.handle.net/10754/563460
Title:
Magneto-mechanical trapping systems for biological target detection
Authors:
Li, Fuquan ( 0000-0002-1573-597X ) ; Kodzius, Rimantas ( 0000-0001-9417-8894 ) ; Gooneratne, Chinthaka Pasan; Foulds, Ian G.; Kosel, Jürgen ( 0000-0002-8998-8275 )
Abstract:
We demonstrate a magnetic microsystem capable of detecting nucleic acids via the size difference between bare magnetic beads and bead compounds. The bead compounds are formed through linking nonmagnetic beads and magnetic beads by the target nucleic acids. The system comprises a tunnel magneto-resistive (TMR) sensor, a trapping well, and a bead-concentrator. The TMR sensor detects the stray field of magnetic beads inside the trapping well, while the sensor output depends on the number of beads. The size of the bead compounds is larger than that of bare magnetic beads, and fewer magnetic beads are required to fill the trapping well. The bead-concentrator, in turn, is capable of filling the trap in a controlled fashion and so to shorten the assay time. The bead-concentrator includes conducting loops surrounding the trapping well and a conducting line underneath. The central conducting line serves to attract magnetic beads in the trapping well and provides a magnetic field to magnetize them so to make them detectable by the TMR sensor. This system excels by its simplicity in that the DNA is incubated with magnetic and nonmagnetic beads, and the solution is then applied to the chip and analyzed in a single step. In current experiments, a signal-to-noise ratio of 40.3 dB was obtained for a solution containing 20.8 nM of DNA. The sensitivity and applicability of this method can be controlled by the size or concentration of the nonmagnetic bead, or by the dimension of the trapping well.
KAUST Department:
Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division; Computational Bioscience Research Center (CBRC); Electrical Engineering Program; Sensing, Magnetism and Microsystems Lab
Publisher:
Springer Science + Business Media
Journal:
Microchimica Acta
Issue Date:
29-Mar-2014
DOI:
10.1007/s00604-014-1241-6
Type:
Article
ISSN:
00263672
Appears in Collections:
Articles; Electrical Engineering Program; Sensing, Magnetism and Microsystems Lab; Computational Bioscience Research Center (CBRC); Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorLi, Fuquanen
dc.contributor.authorKodzius, Rimantasen
dc.contributor.authorGooneratne, Chinthaka Pasanen
dc.contributor.authorFoulds, Ian G.en
dc.contributor.authorKosel, Jürgenen
dc.date.accessioned2015-08-03T11:52:03Zen
dc.date.available2015-08-03T11:52:03Zen
dc.date.issued2014-03-29en
dc.identifier.issn00263672en
dc.identifier.doi10.1007/s00604-014-1241-6en
dc.identifier.urihttp://hdl.handle.net/10754/563460en
dc.description.abstractWe demonstrate a magnetic microsystem capable of detecting nucleic acids via the size difference between bare magnetic beads and bead compounds. The bead compounds are formed through linking nonmagnetic beads and magnetic beads by the target nucleic acids. The system comprises a tunnel magneto-resistive (TMR) sensor, a trapping well, and a bead-concentrator. The TMR sensor detects the stray field of magnetic beads inside the trapping well, while the sensor output depends on the number of beads. The size of the bead compounds is larger than that of bare magnetic beads, and fewer magnetic beads are required to fill the trapping well. The bead-concentrator, in turn, is capable of filling the trap in a controlled fashion and so to shorten the assay time. The bead-concentrator includes conducting loops surrounding the trapping well and a conducting line underneath. The central conducting line serves to attract magnetic beads in the trapping well and provides a magnetic field to magnetize them so to make them detectable by the TMR sensor. This system excels by its simplicity in that the DNA is incubated with magnetic and nonmagnetic beads, and the solution is then applied to the chip and analyzed in a single step. In current experiments, a signal-to-noise ratio of 40.3 dB was obtained for a solution containing 20.8 nM of DNA. The sensitivity and applicability of this method can be controlled by the size or concentration of the nonmagnetic bead, or by the dimension of the trapping well.en
dc.publisherSpringer Science + Business Mediaen
dc.subjectMagnetic beadsen
dc.subjectMagnetic biosensoren
dc.subjectNucleic acid detectionen
dc.subjectTrapping wellen
dc.titleMagneto-mechanical trapping systems for biological target detectionen
dc.typeArticleen
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Divisionen
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.contributor.departmentElectrical Engineering Programen
dc.contributor.departmentSensing, Magnetism and Microsystems Laben
dc.identifier.journalMicrochimica Actaen
kaust.authorKodzius, Rimantasen
kaust.authorGooneratne, Chinthaka Pasanen
kaust.authorFoulds, Ian G.en
kaust.authorKosel, Jürgenen
kaust.authorLi, Fuquanen
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