A DNA origami nanorobot controlled by nucleic acid hybridization

Handle URI:
http://hdl.handle.net/10754/563449
Title:
A DNA origami nanorobot controlled by nucleic acid hybridization
Authors:
Torelli, Emanuela; Marini, Monica ( 0000-0001-8182-5239 ) ; Palmano, Sabrina; Piantanida, Luca; Polano, Cesare; Scarpellini, Alice; Lazzarino, Marco; Firrao, Giuseppe
Abstract:
A prototype for a DNA origami nanorobot is designed, produced, and tested. The cylindrical nanorobot (diameter of 14 nm and length of 48 nm) with a switchable flap, is able to respond to an external stimulus and reacts by a physical switch from a disarmed to an armed configuration able to deliver a cellular compatible message. In the tested design the robot weapon is a nucleic acid fully contained in the inner of the tube and linked to a single point of the internal face of the flap. Upon actuation the nanorobot moves the flap extracting the nucleic acid that assembles into a hemin/G-quadruplex horseradish peroxidase mimicking DNAzyme catalyzing a colorimetric reaction or chemiluminescence generation. The actuation switch is triggered by an external nucleic acid (target) that interacts with a complementary nucleic acid that is beard externally by the nanorobot (probe). Hybridization of probe and target produces a localized structural change that results in flap opening. The flap movement is studied on a two-dimensional prototype origami using Förster resonance energy transfer and is shown to be triggered by a variety of targets, including natural RNAs. The nanorobot has potential for in vivo biosensing and intelligent delivery of biological activators. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
KAUST Department:
Physical Sciences and Engineering (PSE) Division; Materials Science and Engineering Program
Publisher:
Wiley-Blackwell
Journal:
Small
Issue Date:
20-Mar-2014
DOI:
10.1002/smll.201400245
Type:
Article
ISSN:
16136810
Appears in Collections:
Articles; Physical Sciences and Engineering (PSE) Division; Materials Science and Engineering Program

Full metadata record

DC FieldValue Language
dc.contributor.authorTorelli, Emanuelaen
dc.contributor.authorMarini, Monicaen
dc.contributor.authorPalmano, Sabrinaen
dc.contributor.authorPiantanida, Lucaen
dc.contributor.authorPolano, Cesareen
dc.contributor.authorScarpellini, Aliceen
dc.contributor.authorLazzarino, Marcoen
dc.contributor.authorFirrao, Giuseppeen
dc.date.accessioned2015-08-03T11:51:46Zen
dc.date.available2015-08-03T11:51:46Zen
dc.date.issued2014-03-20en
dc.identifier.issn16136810en
dc.identifier.doi10.1002/smll.201400245en
dc.identifier.urihttp://hdl.handle.net/10754/563449en
dc.description.abstractA prototype for a DNA origami nanorobot is designed, produced, and tested. The cylindrical nanorobot (diameter of 14 nm and length of 48 nm) with a switchable flap, is able to respond to an external stimulus and reacts by a physical switch from a disarmed to an armed configuration able to deliver a cellular compatible message. In the tested design the robot weapon is a nucleic acid fully contained in the inner of the tube and linked to a single point of the internal face of the flap. Upon actuation the nanorobot moves the flap extracting the nucleic acid that assembles into a hemin/G-quadruplex horseradish peroxidase mimicking DNAzyme catalyzing a colorimetric reaction or chemiluminescence generation. The actuation switch is triggered by an external nucleic acid (target) that interacts with a complementary nucleic acid that is beard externally by the nanorobot (probe). Hybridization of probe and target produces a localized structural change that results in flap opening. The flap movement is studied on a two-dimensional prototype origami using Förster resonance energy transfer and is shown to be triggered by a variety of targets, including natural RNAs. The nanorobot has potential for in vivo biosensing and intelligent delivery of biological activators. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.en
dc.publisherWiley-Blackwellen
dc.subjectDNA origamien
dc.subjectDNAzyme-based sensorsen
dc.subjecthemin/G-quadruplexen
dc.subjectnanorobotsen
dc.subjectswitchable nanodevicesen
dc.titleA DNA origami nanorobot controlled by nucleic acid hybridizationen
dc.typeArticleen
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.contributor.departmentMaterials Science and Engineering Programen
dc.identifier.journalSmallen
dc.contributor.institutionDepartment of Agricultural and Environmental Sciences, University of Udine, via delle Scienze 206, 33100 Udine, Italyen
dc.contributor.institutionItalian Institute of Technology (IIT), via Morego 30, 16163 Genova, Italyen
dc.contributor.institutionInstitute of Plant Virology (IVV), National Research Council, Strada delle Cacce 73, 10135 Torino, Italyen
dc.contributor.institutionIOM-CNR National Laboratory TASC, Basovizza 34149 Trieste, Italyen
kaust.authorMarini, Monicaen
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