Localization of iron in rice grain using synchrotron X-ray fluorescence microscopy and high resolution secondary ion mass spectrometry

Handle URI:
http://hdl.handle.net/10754/563417
Title:
Localization of iron in rice grain using synchrotron X-ray fluorescence microscopy and high resolution secondary ion mass spectrometry
Authors:
Kyriacou, Bianca; Moore, Katie L.; Paterson, David J.; De Jonge, Martin Daly; Howard, Daryl Lloyd; Stangoulis, James Constantine R; Tester, Mark A. ( 0000-0002-5085-8801 ) ; Lombi, E.; Johnson, Alexander A T
Abstract:
Cereal crops accumulate low levels of iron (Fe) of which only a small fraction (5-10%) is bioavailable in human diets. Extensive co-localization of Fe in outer grain tissues with phytic acid, a strong chelator of metal ions, results in the formation of insoluble complexes that cannot be digested by humans. Here we describe the use of synchrotron X-ray fluorescence microscopy (XFM) and high resolution secondary ion mass spectrometry (NanoSIMS) to map the distribution of Fe, zinc (Zn), phosphorus (P) and other elements in the aleurone and subaleurone layers of mature grain from wild-type and an Fe-enriched line of rice (Oryza sativa L.). The results obtained from both XFM and NanoSIMS indicated that most Fe was co-localized with P (indicative of phytic acid) in the aleurone layer but that a small amount of Fe, often present as "hotspots", extended further into the subaleurone and outer endosperm in a pattern that was not co-localized with P. We hypothesize that Fe in subaleurone and outer endosperm layers of rice grain could be bound to low molecular weight chelators such as nicotianamine and/or deoxymugineic acid. © 2014.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Center for Desert Agriculture; Bioscience Program; The Salt Lab
Publisher:
Elsevier BV
Journal:
Journal of Cereal Science
Issue Date:
Mar-2014
DOI:
10.1016/j.jcs.2013.12.006
Type:
Article
ISSN:
07335210
Appears in Collections:
Articles; Bioscience Program; Center for Desert Agriculture; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorKyriacou, Biancaen
dc.contributor.authorMoore, Katie L.en
dc.contributor.authorPaterson, David J.en
dc.contributor.authorDe Jonge, Martin Dalyen
dc.contributor.authorHoward, Daryl Lloyden
dc.contributor.authorStangoulis, James Constantine Ren
dc.contributor.authorTester, Mark A.en
dc.contributor.authorLombi, E.en
dc.contributor.authorJohnson, Alexander A Ten
dc.date.accessioned2015-08-03T11:50:56Zen
dc.date.available2015-08-03T11:50:56Zen
dc.date.issued2014-03en
dc.identifier.issn07335210en
dc.identifier.doi10.1016/j.jcs.2013.12.006en
dc.identifier.urihttp://hdl.handle.net/10754/563417en
dc.description.abstractCereal crops accumulate low levels of iron (Fe) of which only a small fraction (5-10%) is bioavailable in human diets. Extensive co-localization of Fe in outer grain tissues with phytic acid, a strong chelator of metal ions, results in the formation of insoluble complexes that cannot be digested by humans. Here we describe the use of synchrotron X-ray fluorescence microscopy (XFM) and high resolution secondary ion mass spectrometry (NanoSIMS) to map the distribution of Fe, zinc (Zn), phosphorus (P) and other elements in the aleurone and subaleurone layers of mature grain from wild-type and an Fe-enriched line of rice (Oryza sativa L.). The results obtained from both XFM and NanoSIMS indicated that most Fe was co-localized with P (indicative of phytic acid) in the aleurone layer but that a small amount of Fe, often present as "hotspots", extended further into the subaleurone and outer endosperm in a pattern that was not co-localized with P. We hypothesize that Fe in subaleurone and outer endosperm layers of rice grain could be bound to low molecular weight chelators such as nicotianamine and/or deoxymugineic acid. © 2014.en
dc.publisherElsevier BVen
dc.subjectBioavailabilityen
dc.subjectBiofortificationen
dc.subjectIP6en
dc.subjectPhytateen
dc.titleLocalization of iron in rice grain using synchrotron X-ray fluorescence microscopy and high resolution secondary ion mass spectrometryen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentCenter for Desert Agricultureen
dc.contributor.departmentBioscience Programen
dc.contributor.departmentThe Salt Laben
dc.identifier.journalJournal of Cereal Scienceen
dc.contributor.institutionSchool of Biological Sciences, Flinders University of South Australia, GPO Box 2100, SA 5001, Australiaen
dc.contributor.institutionDepartment of Materials, University of Oxford, Parks Road, Oxford OX1 3PH, United Kingdomen
dc.contributor.institutionAustralian Synchrotron, X-ray Fluorescence Microscopy, 800 Blackburn Road, Clayton, VIC 3168, Australiaen
dc.contributor.institutionCentre for Environmental Risk Assessment and Remediation, University of South Australia, Building X, Mawson Lakes Campus, Mawson Lakes, SA 5095, Australiaen
dc.contributor.institutionAustralian Centre for Plant Functional Genomics, University of Adelaide, PMB1, Glen Osmond, SA 5064, Australiaen
dc.contributor.institutionSchool of Botany, University of Melbourne, Victoria 3010, Australiaen
kaust.authorTester, Mark A.en
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