Multifunctional substrates of thin porous alumina for cell biosensors

Handle URI:
http://hdl.handle.net/10754/563406
Title:
Multifunctional substrates of thin porous alumina for cell biosensors
Authors:
Toccafondi, Chiara; Thorat, Sanjay B.; La Rocca, Rosanna; Scarpellini, Alice; Salerno, Marco; Dante, Silvia; Das, Gobind ( 0000-0003-0942-681X )
Abstract:
We have fabricated anodic porous alumina from thin films (100/500 nm) of aluminium deposited on technological substrates of silicon/glass, and investigated the feasibility of this material as a surface for the development of analytical biosensors aiming to assess the status of living cells. To this goal, porous alumina surfaces with fixed pitch and variable pore size were analyzed for various functionalities. Gold coated (about 25 nm) alumina revealed surface enhanced Raman scattering increasing with the decrease in wall thickness, with factor up to values of approximately 104 with respect to the flat gold surface. Bare porous alumina was employed for micro-patterning and observation via fluorescence images of dye molecules, which demonstrated the surface capability for a drug-loading device. NIH-3T3 fibroblast cells were cultured in vitro and examined after 2 days since seeding, and no significant (P > 0.05) differences in their proliferation were observed on porous and non-porous materials. The effect on cell cultures of pore size in the range of 50–130 nm—with pore pitch of about 250 nm—showed no significant differences in cell viability and similar levels in all cases as on a control substrate. Future work will address combination of all above capabilities into a single device.
KAUST Department:
Physical Sciences and Engineering (PSE) Division
Publisher:
Springer Science + Business Media
Journal:
Journal of Materials Science: Materials in Medicine
Issue Date:
27-Feb-2014
DOI:
10.1007/s10856-014-5178-4
Type:
Article
ISSN:
09574530
Appears in Collections:
Articles; Physical Sciences and Engineering (PSE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorToccafondi, Chiaraen
dc.contributor.authorThorat, Sanjay B.en
dc.contributor.authorLa Rocca, Rosannaen
dc.contributor.authorScarpellini, Aliceen
dc.contributor.authorSalerno, Marcoen
dc.contributor.authorDante, Silviaen
dc.contributor.authorDas, Gobinden
dc.date.accessioned2015-08-03T11:47:47Zen
dc.date.available2015-08-03T11:47:47Zen
dc.date.issued2014-02-27en
dc.identifier.issn09574530en
dc.identifier.doi10.1007/s10856-014-5178-4en
dc.identifier.urihttp://hdl.handle.net/10754/563406en
dc.description.abstractWe have fabricated anodic porous alumina from thin films (100/500 nm) of aluminium deposited on technological substrates of silicon/glass, and investigated the feasibility of this material as a surface for the development of analytical biosensors aiming to assess the status of living cells. To this goal, porous alumina surfaces with fixed pitch and variable pore size were analyzed for various functionalities. Gold coated (about 25 nm) alumina revealed surface enhanced Raman scattering increasing with the decrease in wall thickness, with factor up to values of approximately 104 with respect to the flat gold surface. Bare porous alumina was employed for micro-patterning and observation via fluorescence images of dye molecules, which demonstrated the surface capability for a drug-loading device. NIH-3T3 fibroblast cells were cultured in vitro and examined after 2 days since seeding, and no significant (P > 0.05) differences in their proliferation were observed on porous and non-porous materials. The effect on cell cultures of pore size in the range of 50–130 nm—with pore pitch of about 250 nm—showed no significant differences in cell viability and similar levels in all cases as on a control substrate. Future work will address combination of all above capabilities into a single device.en
dc.publisherSpringer Science + Business Mediaen
dc.titleMultifunctional substrates of thin porous alumina for cell biosensorsen
dc.typeArticleen
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.identifier.journalJournal of Materials Science: Materials in Medicineen
dc.contributor.institutionNanophysics, Istituto Italiano di Tecnologia, via Morego 30Genoa, Italyen
dc.contributor.institutionNanostructures, Istituto Italiano di Tecnologia, via Morego 30Genoa, Italyen
dc.contributor.institutionUniversita‘ di Genova, viale Causa 13Genoa, Italyen
dc.contributor.institutionNanochemistry, Istituto Italiano di Tecnologia, via Morego 30Genoa, Italyen
kaust.authorDas, Gobinden
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