mir-300 promotes self-renewal and inhibits the differentiation of glioma stem-like cells

Handle URI:
http://hdl.handle.net/10754/563358
Title:
mir-300 promotes self-renewal and inhibits the differentiation of glioma stem-like cells
Authors:
Zhang, Daming; Yang, Guang; Chen, Xin; Li, Chunmei; Wang, Lu; Liu, Yaohua; Han, Dayong; Liu, Huailei; Hou, Xu; Zhang, Weiguang; Li, Chenguang; Han, Zhanqiang; Gao, Xin ( 0000-0002-7108-3574 ) ; Zhao, Shiguang
Abstract:
MicroRNAs (miRNAs) are small noncoding RNAs that have been critically implicated in several human cancers. miRNAs are thought to participate in various biological processes, including proliferation, cell cycle, apoptosis, and even the regulation of the stemness properties of cancer stem cells. In this study, we explore the potential role of miR-300 in glioma stem-like cells (GSLCs). We isolated GSLCs from glioma biopsy specimens and identified the stemness properties of the cells through neurosphere formation assays, multilineage differentiation ability analysis, and immunofluorescence analysis of glioma stem cell markers. We found that miR-300 is commonly upregulated in glioma tissues, and the expression of miR-300 was higher in GSLCs. The results of functional experiments demonstrated that miR-300 can enhance the self-renewal of GSLCs and reduce differentiation toward both astrocyte and neural fates. In addition, LZTS2 is a direct target of miR-300. In conclusion, our results demonstrate the critical role of miR-300 in GSLCs and its functions in LZTS2 inhibition and describe a new approach for the molecular regulation of tumor stem cells. © 2014 Springer Science+Business Media.
KAUST Department:
Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division; Computer Science Program; Computational Bioscience Research Center (CBRC); Structural and Functional Bioinformatics Group
Publisher:
Springer Science + Business Media
Journal:
Journal of Molecular Neuroscience
Issue Date:
28-Jan-2014
DOI:
10.1007/s12031-014-0230-x
Type:
Article
ISSN:
08958696
Sponsors:
This work was supported by the National Natural Science Foundation of China (grant numbers 81272788 and 81302178), the Natural Science Foundation of Heilongjiang (QC2013C096), and the Fund of the First Affiliated Hospital of Harbin Medical University (2013B01).
Appears in Collections:
Articles; Structural and Functional Bioinformatics Group; Computer Science Program; Computational Bioscience Research Center (CBRC); Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorZhang, Damingen
dc.contributor.authorYang, Guangen
dc.contributor.authorChen, Xinen
dc.contributor.authorLi, Chunmeien
dc.contributor.authorWang, Luen
dc.contributor.authorLiu, Yaohuaen
dc.contributor.authorHan, Dayongen
dc.contributor.authorLiu, Huaileien
dc.contributor.authorHou, Xuen
dc.contributor.authorZhang, Weiguangen
dc.contributor.authorLi, Chenguangen
dc.contributor.authorHan, Zhanqiangen
dc.contributor.authorGao, Xinen
dc.contributor.authorZhao, Shiguangen
dc.date.accessioned2015-08-03T11:46:37Zen
dc.date.available2015-08-03T11:46:37Zen
dc.date.issued2014-01-28en
dc.identifier.issn08958696en
dc.identifier.doi10.1007/s12031-014-0230-xen
dc.identifier.urihttp://hdl.handle.net/10754/563358en
dc.description.abstractMicroRNAs (miRNAs) are small noncoding RNAs that have been critically implicated in several human cancers. miRNAs are thought to participate in various biological processes, including proliferation, cell cycle, apoptosis, and even the regulation of the stemness properties of cancer stem cells. In this study, we explore the potential role of miR-300 in glioma stem-like cells (GSLCs). We isolated GSLCs from glioma biopsy specimens and identified the stemness properties of the cells through neurosphere formation assays, multilineage differentiation ability analysis, and immunofluorescence analysis of glioma stem cell markers. We found that miR-300 is commonly upregulated in glioma tissues, and the expression of miR-300 was higher in GSLCs. The results of functional experiments demonstrated that miR-300 can enhance the self-renewal of GSLCs and reduce differentiation toward both astrocyte and neural fates. In addition, LZTS2 is a direct target of miR-300. In conclusion, our results demonstrate the critical role of miR-300 in GSLCs and its functions in LZTS2 inhibition and describe a new approach for the molecular regulation of tumor stem cells. © 2014 Springer Science+Business Media.en
dc.description.sponsorshipThis work was supported by the National Natural Science Foundation of China (grant numbers 81272788 and 81302178), the Natural Science Foundation of Heilongjiang (QC2013C096), and the Fund of the First Affiliated Hospital of Harbin Medical University (2013B01).en
dc.publisherSpringer Science + Business Mediaen
dc.subjectDifferentiationen
dc.subjectGlioma stem-like cellsen
dc.subjectLZTS2en
dc.subjectmiR-300en
dc.subjectSelf-renewalen
dc.titlemir-300 promotes self-renewal and inhibits the differentiation of glioma stem-like cellsen
dc.typeArticleen
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Divisionen
dc.contributor.departmentComputer Science Programen
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.contributor.departmentStructural and Functional Bioinformatics Groupen
dc.identifier.journalJournal of Molecular Neuroscienceen
dc.contributor.institutionDepartment of Neurosurgery, Harbin Medical University, Youzheng Street No. 23, Nangang District, Harbin, Heilongjiang Province 150001, Chinaen
dc.contributor.institutionInstitute of Brain Science, Harbin Medical University, Harbin, Heilongjiang, Chinaen
dc.contributor.institutionDepartment of Neurology, Harbin Medical University, Harbin, Heilongjiang, Chinaen
dc.contributor.institutionDepartment of Pharmacology (State-Province Key Laboratories of Biomedicine-Pharmaceutics of China), Harbin Medical University, Harbin, Heilongjiang, Chinaen
kaust.authorGao, Xinen
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