The chloroplast genome of a symbiodinium sp. clade C3 isolate

Handle URI:
http://hdl.handle.net/10754/563301
Title:
The chloroplast genome of a symbiodinium sp. clade C3 isolate
Authors:
Barbrook, Adrian C.; Voolstra, Christian R. ( 0000-0003-4555-3795 ) ; Howe, Christopher J.
Abstract:
Dinoflagellate algae of the genus Symbiodinium form important symbioses within corals and other benthic marine animals. Dinoflagellates possess an extremely reduced plastid genome relative to those examined in plants and other algae. In dinoflagellates the plastid genes are located on small plasmids, commonly referred to as 'minicircles'. However, the chloroplast genomes of dinoflagellates have only been extensively characterised from a handful of species. There is also evidence of considerable variation in the chloroplast genome organisation across those species that have been examined. We therefore characterised the chloroplast genome from an environmental coral isolate, in this case containing a symbiont belonging to the Symbiodinium sp. clade C3. The gene content of the genome is well conserved with respect to previously characterised genomes. However, unlike previously characterised dinoflagellate chloroplast genomes we did not identify any 'empty' minicircles. The sequences of this chloroplast genome show a high rate of evolution relative to other algal species. Particularly notable was a surprisingly high level of sequence divergence within the core polypeptides of photosystem I, the reasons for which are currently unknown. This chloroplast genome also possesses distinctive codon usage and GC content. These features suggest that chloroplast genomes in Symbiodinium are highly plastic. © 2013 Adrian C. Barbrook.
KAUST Department:
Red Sea Research Center (RSRC); Biological and Environmental Sciences and Engineering (BESE) Division; Marine Science Program; Reef Genomics Lab
Publisher:
Elsevier BV
Journal:
Protist
Issue Date:
Jan-2014
DOI:
10.1016/j.protis.2013.09.006
PubMed ID:
24316380
Type:
Article
ISSN:
14344610
Sponsors:
The authors wish to thank Professor Angela Douglas for the gift of the DNA extract used in this study. The authors also wish to thank Daniel J. Thornhilland Todd J. LaJeunesse for providing assistance inphylogenetic assignment of the Symbiodinium isolate. This work was funded through a grant from the Cambridge - KAUST Academic Excellence Alliance (AEA) program (award number 7000000056) as well as by the Leverhulme Trust.
Appears in Collections:
Articles; Red Sea Research Center (RSRC); Marine Science Program; Biological and Environmental Sciences and Engineering (BESE) Division; Reef Genomics, part of the Global Ocean Genome Project

Full metadata record

DC FieldValue Language
dc.contributor.authorBarbrook, Adrian C.en
dc.contributor.authorVoolstra, Christian R.en
dc.contributor.authorHowe, Christopher J.en
dc.date.accessioned2015-08-03T11:45:14Zen
dc.date.available2015-08-03T11:45:14Zen
dc.date.issued2014-01en
dc.identifier.issn14344610en
dc.identifier.pmid24316380en
dc.identifier.doi10.1016/j.protis.2013.09.006en
dc.identifier.urihttp://hdl.handle.net/10754/563301en
dc.description.abstractDinoflagellate algae of the genus Symbiodinium form important symbioses within corals and other benthic marine animals. Dinoflagellates possess an extremely reduced plastid genome relative to those examined in plants and other algae. In dinoflagellates the plastid genes are located on small plasmids, commonly referred to as 'minicircles'. However, the chloroplast genomes of dinoflagellates have only been extensively characterised from a handful of species. There is also evidence of considerable variation in the chloroplast genome organisation across those species that have been examined. We therefore characterised the chloroplast genome from an environmental coral isolate, in this case containing a symbiont belonging to the Symbiodinium sp. clade C3. The gene content of the genome is well conserved with respect to previously characterised genomes. However, unlike previously characterised dinoflagellate chloroplast genomes we did not identify any 'empty' minicircles. The sequences of this chloroplast genome show a high rate of evolution relative to other algal species. Particularly notable was a surprisingly high level of sequence divergence within the core polypeptides of photosystem I, the reasons for which are currently unknown. This chloroplast genome also possesses distinctive codon usage and GC content. These features suggest that chloroplast genomes in Symbiodinium are highly plastic. © 2013 Adrian C. Barbrook.en
dc.description.sponsorshipThe authors wish to thank Professor Angela Douglas for the gift of the DNA extract used in this study. The authors also wish to thank Daniel J. Thornhilland Todd J. LaJeunesse for providing assistance inphylogenetic assignment of the Symbiodinium isolate. This work was funded through a grant from the Cambridge - KAUST Academic Excellence Alliance (AEA) program (award number 7000000056) as well as by the Leverhulme Trust.en
dc.publisherElsevier BVen
dc.subjectChloroplast genomeen
dc.subjectDinoflagellateen
dc.subjectDiversity.en
dc.subjectMinicircleen
dc.subjectSymbiodiniumen
dc.titleThe chloroplast genome of a symbiodinium sp. clade C3 isolateen
dc.typeArticleen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentMarine Science Programen
dc.contributor.departmentReef Genomics Laben
dc.identifier.journalProtisten
dc.contributor.institutionDepartment of Biochemistry, University of Cambridge, Building O, Downing Site, Tennis Court Road, Cambridge, CB2 1QW, United Kingdomen
kaust.authorVoolstra, Christian R.en

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