High throughput second harmonic imaging for label-free biological applications

Handle URI:
http://hdl.handle.net/10754/563269
Title:
High throughput second harmonic imaging for label-free biological applications
Authors:
Macias Romero, Carlos; Didier, Marie E P; Jourdain, Pascal; Marquet, Pierre; Magistretti, Pierre J. ( 0000-0002-6678-320X ) ; Tarun, Orly B.; Zubkovs, Vitalijs; Radenovic, Aleksandra; Roke, Sylvie
Abstract:
Second harmonic generation (SHG) is inherently sensitive to the absence of spatial centrosymmetry, which can render it intrinsically sensitive to interfacial processes, chemical changes and electrochemical responses. Here, we seek to improve the imaging throughput of SHG microscopy by using a wide-field imaging scheme in combination with a medium-range repetition rate amplified near infrared femtosecond laser source and gated detection. The imaging throughput of this configuration is tested by measuring the optical image contrast for different image acquisition times of BaTiO3 nanoparticles in two different wide-field setups and one commercial point-scanning configuration. We find that the second harmonic imaging throughput is improved by 2-3 orders of magnitude compared to point-scan imaging. Capitalizing on this result, we perform low fluence imaging of (parts of) living mammalian neurons in culture.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program
Publisher:
The Optical Society
Journal:
Optics Express
Issue Date:
2014
DOI:
10.1364/OE.22.031102
Type:
Article
ISSN:
10944087
Sponsors:
This work was supported by the Julia Jacobi Foundation, the Swiss National Foundation (grant number 200021-146884), and European Commission, Research Executive Agency Marie Curie Actions 'FINON' (ITN-2013-607842). We would like to thank Prof. K. B. Eisenthal for very inspiring discussions.
Appears in Collections:
Articles; Bioscience Program; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorMacias Romero, Carlosen
dc.contributor.authorDidier, Marie E Pen
dc.contributor.authorJourdain, Pascalen
dc.contributor.authorMarquet, Pierreen
dc.contributor.authorMagistretti, Pierre J.en
dc.contributor.authorTarun, Orly B.en
dc.contributor.authorZubkovs, Vitalijsen
dc.contributor.authorRadenovic, Aleksandraen
dc.contributor.authorRoke, Sylvieen
dc.date.accessioned2015-08-03T11:44:31Zen
dc.date.available2015-08-03T11:44:31Zen
dc.date.issued2014en
dc.identifier.issn10944087en
dc.identifier.doi10.1364/OE.22.031102en
dc.identifier.urihttp://hdl.handle.net/10754/563269en
dc.description.abstractSecond harmonic generation (SHG) is inherently sensitive to the absence of spatial centrosymmetry, which can render it intrinsically sensitive to interfacial processes, chemical changes and electrochemical responses. Here, we seek to improve the imaging throughput of SHG microscopy by using a wide-field imaging scheme in combination with a medium-range repetition rate amplified near infrared femtosecond laser source and gated detection. The imaging throughput of this configuration is tested by measuring the optical image contrast for different image acquisition times of BaTiO3 nanoparticles in two different wide-field setups and one commercial point-scanning configuration. We find that the second harmonic imaging throughput is improved by 2-3 orders of magnitude compared to point-scan imaging. Capitalizing on this result, we perform low fluence imaging of (parts of) living mammalian neurons in culture.en
dc.description.sponsorshipThis work was supported by the Julia Jacobi Foundation, the Swiss National Foundation (grant number 200021-146884), and European Commission, Research Executive Agency Marie Curie Actions 'FINON' (ITN-2013-607842). We would like to thank Prof. K. B. Eisenthal for very inspiring discussions.en
dc.publisherThe Optical Societyen
dc.titleHigh throughput second harmonic imaging for label-free biological applicationsen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentBioscience Programen
dc.identifier.journalOptics Expressen
dc.contributor.institutionLaboratory for Fundamental BioPhotonics, Institute of Bioengineering, École Polytechnique Fédérale de Lausanne (EPFL)Lausanne, Switzerlanden
dc.contributor.institutionLaboratory of Neuroenergetics and Cellular Dynamics, Brain Mind Institute, École Polytechnique Fédérale de Lausanne (EPFL)Lausanne, Switzerlanden
dc.contributor.institutionLaboratory of Nanoscale Biology, Institute of Bioengineering, École Polytechnique Fédérale de Lausanne (EPFL)Lausanne, Switzerlanden
kaust.authorMagistretti, Pierre J.en
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