Real time hybridization studies by resonant waveguide gratings using nanopattern imaging for Single Nucleotide Polymorphism detection

Handle URI:
http://hdl.handle.net/10754/563155
Title:
Real time hybridization studies by resonant waveguide gratings using nanopattern imaging for Single Nucleotide Polymorphism detection
Authors:
Bougot-Robin, Kristelle; Kodzius, Rimantas ( 0000-0001-9417-8894 ) ; Yue, Weisheng; Chen, Longqing; Li, Shunbo; Zhang, Xixiang ( 0000-0002-3478-6414 ) ; Bénisty, Henri; Wen, Weijia
Abstract:
2D imaging of biochips is particularly interesting for multiplex biosensing. Resonant properties allow label-free detection using the change of refractive index at the chip surface. We demonstrate a new principle of Scanning Of Resonance on Chip by Imaging (SORCI) based on spatial profiles of nanopatterns of resonant waveguide gratings (RWGs) and its embodiment in a fluidic chip for real-time biological studies. This scheme allows multiplexing of the resonance itself by providing nanopattern sensing areas in a bioarray format. Through several chip designs we discuss resonance spatial profiles, dispersion and electric field distribution for optimal light-matter interaction with biological species of different sizes. Fluidic integration is carried out with a black anodized aluminum chamber, advantageous in term of mechanical stability, multiple uses of the chip, temperature control and low optical background. Real-time hybridization experiments are illustrated by SNP (Single Nucleotide Polymorphism) detection in gyrase A of E. coli K12, observed in evolution studies of resistance to the antibiotic ciprofloxacin. We choose a 100 base pairs (bp) DNA target (∼30 kDa) including the codon of interest and demonstrate the high specificity of our technique for probes and targets with close affinity constants. This work validates the safe applicability of our unique combination of RWGs and simple instrumentation for real-time biosensing with sensitivity in buffer solution of ∼10 pg/mm2. Paralleling the success of RWGs sensing for cells sensing, our work opens new avenues for a large number of biological studies. © 2013 Springer Science+Business Media.
KAUST Department:
KAUST-HKUST Micro/Nanofluidic Joint Laboratory; Advanced Nanofabrication, Imaging and Characterization Core Lab; Computational Bioscience Research Center (CBRC); Physical Sciences and Engineering (PSE) Division; Materials Science and Engineering Program; Core Labs
Publisher:
Springer Nature
Journal:
Biomedical Microdevices
Issue Date:
20-Dec-2013
DOI:
10.1007/s10544-013-9832-2
PubMed ID:
24357005
Type:
Article
ISSN:
13872176
Sponsors:
The authors acknowledge L. Wang, X. Xiao, Boon S. Ooi, Q. Zhang and R. H. Austin for fruitful discussion. We thank as well HKUST Nanofabrication facilities staff for their help in chip fabrication process. The electron beam lithography project is supported by University Grants Committee reference SEG_HKUST10. The project is supported by RGC grant number 674710, as well as grant RPC11SC01.
Appears in Collections:
Articles; Advanced Nanofabrication, Imaging and Characterization Core Lab; Physical Sciences and Engineering (PSE) Division; Materials Science and Engineering Program; Computational Bioscience Research Center (CBRC)

Full metadata record

DC FieldValue Language
dc.contributor.authorBougot-Robin, Kristelleen
dc.contributor.authorKodzius, Rimantasen
dc.contributor.authorYue, Weishengen
dc.contributor.authorChen, Longqingen
dc.contributor.authorLi, Shunboen
dc.contributor.authorZhang, Xixiangen
dc.contributor.authorBénisty, Henrien
dc.contributor.authorWen, Weijiaen
dc.date.accessioned2015-08-03T11:37:04Zen
dc.date.available2015-08-03T11:37:04Zen
dc.date.issued2013-12-20en
dc.identifier.issn13872176en
dc.identifier.pmid24357005en
dc.identifier.doi10.1007/s10544-013-9832-2en
dc.identifier.urihttp://hdl.handle.net/10754/563155en
dc.description.abstract2D imaging of biochips is particularly interesting for multiplex biosensing. Resonant properties allow label-free detection using the change of refractive index at the chip surface. We demonstrate a new principle of Scanning Of Resonance on Chip by Imaging (SORCI) based on spatial profiles of nanopatterns of resonant waveguide gratings (RWGs) and its embodiment in a fluidic chip for real-time biological studies. This scheme allows multiplexing of the resonance itself by providing nanopattern sensing areas in a bioarray format. Through several chip designs we discuss resonance spatial profiles, dispersion and electric field distribution for optimal light-matter interaction with biological species of different sizes. Fluidic integration is carried out with a black anodized aluminum chamber, advantageous in term of mechanical stability, multiple uses of the chip, temperature control and low optical background. Real-time hybridization experiments are illustrated by SNP (Single Nucleotide Polymorphism) detection in gyrase A of E. coli K12, observed in evolution studies of resistance to the antibiotic ciprofloxacin. We choose a 100 base pairs (bp) DNA target (∼30 kDa) including the codon of interest and demonstrate the high specificity of our technique for probes and targets with close affinity constants. This work validates the safe applicability of our unique combination of RWGs and simple instrumentation for real-time biosensing with sensitivity in buffer solution of ∼10 pg/mm2. Paralleling the success of RWGs sensing for cells sensing, our work opens new avenues for a large number of biological studies. © 2013 Springer Science+Business Media.en
dc.description.sponsorshipThe authors acknowledge L. Wang, X. Xiao, Boon S. Ooi, Q. Zhang and R. H. Austin for fruitful discussion. We thank as well HKUST Nanofabrication facilities staff for their help in chip fabrication process. The electron beam lithography project is supported by University Grants Committee reference SEG_HKUST10. The project is supported by RGC grant number 674710, as well as grant RPC11SC01.en
dc.publisherSpringer Natureen
dc.subjectBioarray imagingen
dc.subjectLabel-freeen
dc.subjectOptofluidicen
dc.subjectReal-timeen
dc.subjectResonant waveguide gratingen
dc.subjectSingle Nucleotide Polymorphismen
dc.titleReal time hybridization studies by resonant waveguide gratings using nanopattern imaging for Single Nucleotide Polymorphism detectionen
dc.typeArticleen
dc.contributor.departmentKAUST-HKUST Micro/Nanofluidic Joint Laboratoryen
dc.contributor.departmentAdvanced Nanofabrication, Imaging and Characterization Core Laben
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.contributor.departmentMaterials Science and Engineering Programen
dc.contributor.departmentCore Labsen
dc.identifier.journalBiomedical Microdevicesen
dc.contributor.institutionHong Kong Univ Sci & Technol, Inst Adv Study, Kowloon, Hong Kong, Peoples R Chinaen
dc.contributor.institutionHong Kong Univ Sci & Technol, Dept Phys, Kowloon, Hong Kong, Peoples R Chinaen
dc.contributor.institutionUniv Paris Sud, CNRS, Inst Opt, Lab Charles Fabry,Grad Sch, F-91127 Palaiseau, Franceen
kaust.authorKodzius, Rimantasen
kaust.authorYue, Weishengen
kaust.authorChen, Longqingen
kaust.authorZhang, Xixiangen

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