Expression of the Arabidopsis vacuolar H+-pyrophosphatase gene (AVP1) improves the shoot biomass of transgenic barley and increases grain yield in a saline field

Handle URI:
http://hdl.handle.net/10754/563098
Title:
Expression of the Arabidopsis vacuolar H+-pyrophosphatase gene (AVP1) improves the shoot biomass of transgenic barley and increases grain yield in a saline field
Authors:
Schilling, Rhiannon K.; Marschner, Petra; Shavrukov, Yuri N.; Berger, Bettina; Tester, Mark A. ( 0000-0002-5085-8801 ) ; Roy, Stuart John; Plett, Darren Craig
Abstract:
Cereal varieties with improved salinity tolerance are needed to achieve profitable grain yields in saline soils. The expression of AVP1, an Arabidopsis gene encoding a vacuolar proton pumping pyrophosphatase (H+-PPase), has been shown to improve the salinity tolerance of transgenic plants in greenhouse conditions. However, the potential for this gene to improve the grain yield of cereal crops in a saline field has yet to be evaluated. Recent advances in high-throughput nondestructive phenotyping technologies also offer an opportunity to quantitatively evaluate the growth of transgenic plants under abiotic stress through time. In this study, the growth of transgenic barley expressing AVP1 was evaluated under saline conditions in a pot experiment using nondestructive plant imaging and in a saline field trial. Greenhouse-grown transgenic barley expressing AVP1 produced a larger shoot biomass compared to segregants, as determined by an increase in projected shoot area, when grown in soil with 150 mm NaCl. This increase in shoot biomass of transgenic AVP1 barley occurred from an early growth stage and also in nonsaline conditions. In a saline field, the transgenic barley expressing AVP1 also showed an increase in shoot biomass and, importantly, produced a greater grain yield per plant compared to wild-type plants. Interestingly, the expression of AVP1 did not alter barley leaf sodium concentrations in either greenhouse- or field-grown plants. This study validates our greenhouse-based experiments and indicates that transgenic barley expressing AVP1 is a promising option for increasing cereal crop productivity in saline fields. © 2013 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Center for Desert Agriculture; Bioscience Program; The Salt Lab
Publisher:
Wiley-Blackwell
Journal:
Plant Biotechnology Journal
Issue Date:
22-Nov-2013
DOI:
10.1111/pbi.12145
PubMed ID:
24261956
Type:
Article
ISSN:
14677644
Appears in Collections:
Articles; Bioscience Program; Center for Desert Agriculture; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorSchilling, Rhiannon K.en
dc.contributor.authorMarschner, Petraen
dc.contributor.authorShavrukov, Yuri N.en
dc.contributor.authorBerger, Bettinaen
dc.contributor.authorTester, Mark A.en
dc.contributor.authorRoy, Stuart Johnen
dc.contributor.authorPlett, Darren Craigen
dc.date.accessioned2015-08-03T11:35:43Zen
dc.date.available2015-08-03T11:35:43Zen
dc.date.issued2013-11-22en
dc.identifier.issn14677644en
dc.identifier.pmid24261956en
dc.identifier.doi10.1111/pbi.12145en
dc.identifier.urihttp://hdl.handle.net/10754/563098en
dc.description.abstractCereal varieties with improved salinity tolerance are needed to achieve profitable grain yields in saline soils. The expression of AVP1, an Arabidopsis gene encoding a vacuolar proton pumping pyrophosphatase (H+-PPase), has been shown to improve the salinity tolerance of transgenic plants in greenhouse conditions. However, the potential for this gene to improve the grain yield of cereal crops in a saline field has yet to be evaluated. Recent advances in high-throughput nondestructive phenotyping technologies also offer an opportunity to quantitatively evaluate the growth of transgenic plants under abiotic stress through time. In this study, the growth of transgenic barley expressing AVP1 was evaluated under saline conditions in a pot experiment using nondestructive plant imaging and in a saline field trial. Greenhouse-grown transgenic barley expressing AVP1 produced a larger shoot biomass compared to segregants, as determined by an increase in projected shoot area, when grown in soil with 150 mm NaCl. This increase in shoot biomass of transgenic AVP1 barley occurred from an early growth stage and also in nonsaline conditions. In a saline field, the transgenic barley expressing AVP1 also showed an increase in shoot biomass and, importantly, produced a greater grain yield per plant compared to wild-type plants. Interestingly, the expression of AVP1 did not alter barley leaf sodium concentrations in either greenhouse- or field-grown plants. This study validates our greenhouse-based experiments and indicates that transgenic barley expressing AVP1 is a promising option for increasing cereal crop productivity in saline fields. © 2013 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd.en
dc.publisherWiley-Blackwellen
dc.subjectAVP1en
dc.subjectBarleyen
dc.subjectGM field trialsen
dc.subjectGrain yielden
dc.subjectNondestructive imagingen
dc.subjectSalinityen
dc.titleExpression of the Arabidopsis vacuolar H+-pyrophosphatase gene (AVP1) improves the shoot biomass of transgenic barley and increases grain yield in a saline fielden
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentCenter for Desert Agricultureen
dc.contributor.departmentBioscience Programen
dc.contributor.departmentThe Salt Laben
dc.identifier.journalPlant Biotechnology Journalen
dc.contributor.institutionAustralian Centre for Plant Functional Genomics, Adelaide, SA, Australiaen
dc.contributor.institutionSchool of Agriculture, Food and Wine, The University of Adelaide, Adelaide, SA, Australiaen
dc.contributor.institutionThe Plant Accelerator, Australian Plant Phenomics Facility, The University of Adelaide, Adelaide, SA, Australiaen
kaust.authorTester, Mark A.en

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