A microfluidic device integrating plasmonic nanodevices for Raman spectroscopy analysis on trapped single living cells

Handle URI:
http://hdl.handle.net/10754/563067
Title:
A microfluidic device integrating plasmonic nanodevices for Raman spectroscopy analysis on trapped single living cells
Authors:
Perozziello, Gerardo; Catalano, Rossella; Francardi, Marco; Rondanina, Eliana; Pardeo, Francesca; De Angelis, Francesco De; Malara, Natalia Maria; Candeloro, Patrizio; Morrone, Giovanni; Di Fabrizio, Enzo M. ( 0000-0001-5886-4678 )
Abstract:
In this work we developed a microfluidic device integrating nanoplasmonic devices combined with fluidic trapping regions. The microfuidic traps allow to capture single cells in areas where plasmonic sensors are placed. In this way it is possible to perform Enhanced Raman analysis on the cell membranes. Moreover, by changing direction of the flux it is possible to change the orientation of the cell in the trap, so that it is possible to analyze different points of the membrane of the same cell. We shows an innovative procedure to fabricate and assembly the microfluidic device which combine photolithography, focused ion beam machining, and hybrid bonding between a polymer substrate and lid of Calcium fluoride. This procedure is compatible with the fabrication of the plasmonic sensors in close proximity of the microfluidic traps. Moreover, the use of Calcium fluoride as lid allows full compatibility with Raman measurements producing negligible Raman background signal and avoids Raman artifacts. Finally, we performed Raman analysis on cells to monitor their oxidative stress under particular non physiological conditions. © 2013 Elsevier B.V. All rights reserved.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Physical Sciences and Engineering (PSE) Division; Materials Science and Engineering Program
Publisher:
Elsevier BV
Journal:
Microelectronic Engineering
Issue Date:
Nov-2013
DOI:
10.1016/j.mee.2013.02.023
Type:
Article
ISSN:
01679317
Sponsors:
This work was partially supported by the project PON "Nuove strategie nanotecnologiche per la messa a punto di farmaci e presidi diagnostici diretti verso cellule cancerose circolanti" (cod.PON01_02782, CUP B71H11000870005), the project FIRB "Rete Nazionale di Ricerca sulle Nanoscienze ItalNanoNet" (cod.RBPR05JH2P_010, CUP B41 J09000110005) and Cariplo Foundation under the project "New Frontiers in Plasmonic Nanosensing" (Grant No. 2011-0338) granted to the nanotechnology laboratory of the Department of Experimental Medicine of the University "Magna Graecia" of Catanzaro.
Appears in Collections:
Articles; Physical Sciences and Engineering (PSE) Division; Materials Science and Engineering Program; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorPerozziello, Gerardoen
dc.contributor.authorCatalano, Rossellaen
dc.contributor.authorFrancardi, Marcoen
dc.contributor.authorRondanina, Elianaen
dc.contributor.authorPardeo, Francescaen
dc.contributor.authorDe Angelis, Francesco Deen
dc.contributor.authorMalara, Natalia Mariaen
dc.contributor.authorCandeloro, Patrizioen
dc.contributor.authorMorrone, Giovannien
dc.contributor.authorDi Fabrizio, Enzo M.en
dc.date.accessioned2015-08-03T11:35:01Zen
dc.date.available2015-08-03T11:35:01Zen
dc.date.issued2013-11en
dc.identifier.issn01679317en
dc.identifier.doi10.1016/j.mee.2013.02.023en
dc.identifier.urihttp://hdl.handle.net/10754/563067en
dc.description.abstractIn this work we developed a microfluidic device integrating nanoplasmonic devices combined with fluidic trapping regions. The microfuidic traps allow to capture single cells in areas where plasmonic sensors are placed. In this way it is possible to perform Enhanced Raman analysis on the cell membranes. Moreover, by changing direction of the flux it is possible to change the orientation of the cell in the trap, so that it is possible to analyze different points of the membrane of the same cell. We shows an innovative procedure to fabricate and assembly the microfluidic device which combine photolithography, focused ion beam machining, and hybrid bonding between a polymer substrate and lid of Calcium fluoride. This procedure is compatible with the fabrication of the plasmonic sensors in close proximity of the microfluidic traps. Moreover, the use of Calcium fluoride as lid allows full compatibility with Raman measurements producing negligible Raman background signal and avoids Raman artifacts. Finally, we performed Raman analysis on cells to monitor their oxidative stress under particular non physiological conditions. © 2013 Elsevier B.V. All rights reserved.en
dc.description.sponsorshipThis work was partially supported by the project PON "Nuove strategie nanotecnologiche per la messa a punto di farmaci e presidi diagnostici diretti verso cellule cancerose circolanti" (cod.PON01_02782, CUP B71H11000870005), the project FIRB "Rete Nazionale di Ricerca sulle Nanoscienze ItalNanoNet" (cod.RBPR05JH2P_010, CUP B41 J09000110005) and Cariplo Foundation under the project "New Frontiers in Plasmonic Nanosensing" (Grant No. 2011-0338) granted to the nanotechnology laboratory of the Department of Experimental Medicine of the University "Magna Graecia" of Catanzaro.en
dc.publisherElsevier BVen
dc.subjectCell handlingen
dc.subjectPlasmonic nanodevicesen
dc.subjectSingle molecule detectionen
dc.titleA microfluidic device integrating plasmonic nanodevices for Raman spectroscopy analysis on trapped single living cellsen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.contributor.departmentMaterials Science and Engineering Programen
dc.identifier.journalMicroelectronic Engineeringen
dc.contributor.institutionBioNEM (Bio Nano Engineering and Technology for Medicine) Lab, University Magna Graecia of Catanzaro, Loc. Germaneto, 88100 Catanzaro, Italyen
dc.contributor.institutionItalian Institute of Technology IIT, Via Morego 30, 16163 Genova, Italyen
kaust.authorDi Fabrizio, Enzo M.en
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