Genes involved in the astrocyte-neuron lactate shuttle (ANLS) are specifcally regulated in cortical astrocytes following sleep deprivation in mice

Handle URI:
http://hdl.handle.net/10754/563029
Title:
Genes involved in the astrocyte-neuron lactate shuttle (ANLS) are specifcally regulated in cortical astrocytes following sleep deprivation in mice
Authors:
Petit, Jean Marie; Gyger, Joël; Burlet-Godinot, Sophie; Fiumelli, Hubert ( 0000-0001-5136-3045 ) ; Martin, Jean Luc; Magistretti, Pierre J. ( 0000-0002-6678-320X )
Abstract:
Study Objectives: There is growing evidence indicating that in order to meet the neuronal energy demands, astrocytes provide lactate as an energy substrate for neurons through a mechanism called "astrocyte-neuron lactate shuttle" (ANLS). Since neuronal activity changes dramatically during vigilance states, we hypothesized that the ANLS may be regulated during the sleep-wake cycle. To test this hypothesis we investigated the expression of genes associated with the ANLS specifcally in astrocytes following sleep deprivation. Astrocytes were purifed by fuorescence-activated cell sorting from transgenic mice expressing the green fuorescent protein (GFP) under the control of the human astrocytic GFAP-promoter. Design: 6-hour instrumental sleep deprivation (TSD). Setting: Animal sleep research laboratory. Participants: Young (P23-P27) FVB/N-Tg (GFAP-GFP) 14Mes/J (Tg) mice of both sexes and 7-8 week male Tg and FVB/Nj mice. Interventions: Basal sleep recordings and sleep deprivation achieved using a modifed cage where animals were gently forced to move. Measurements and Results: Since Tg and FVB/Nj mice displayed a similar sleep-wake pattern, we performed a TSD in young Tg mice. Total RNA was extracted from the GFP-positive and GFP-negative cells sorted from cerebral cortex. Quantitative RT-PCR analysis showed that levels of Glut1, a-2-Na/K pump, Glt1, and Ldha mRNAs were signifcantly increased following TSD in GFP-positive cells. In GFP-negative cells, a tendency to increase, although not signifcant, was observed for Ldha, Mct2, and α-3-Na/K pump mRNAs. Conclusions: This study shows that TSD induces the expression of genes associated with ANLS specifcally in astrocytes, underlying the important role of astrocytes in the maintenance of the neuro-metabolic coupling across the sleep-wake cycle.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program
Publisher:
Associated Professional Sleep Societies (APSS)
Journal:
SLEEP
Issue Date:
1-Oct-2013
DOI:
10.5665/sleep.3034
PubMed ID:
24082304
PubMed Central ID:
PMC3773194
Type:
Article
ISSN:
01618105
Sponsors:
The authors warmly thank M. Garcia, G. Tapia, and S. Winkler of the Flow Cytometry Core Facility (Life Sciences Faculty, EPFL, Lausanne, Switzerland) for their technical expertise. This work was supported by a Swiss National Science Foundation grant (3100AO-108336/1) to Dr. Magistretti.
Additional Links:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3773194
Appears in Collections:
Articles; Bioscience Program; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorPetit, Jean Marieen
dc.contributor.authorGyger, Joëlen
dc.contributor.authorBurlet-Godinot, Sophieen
dc.contributor.authorFiumelli, Huberten
dc.contributor.authorMartin, Jean Lucen
dc.contributor.authorMagistretti, Pierre J.en
dc.date.accessioned2015-08-03T11:34:06Zen
dc.date.available2015-08-03T11:34:06Zen
dc.date.issued2013-10-01en
dc.identifier.issn01618105en
dc.identifier.pmid24082304en
dc.identifier.doi10.5665/sleep.3034en
dc.identifier.urihttp://hdl.handle.net/10754/563029en
dc.description.abstractStudy Objectives: There is growing evidence indicating that in order to meet the neuronal energy demands, astrocytes provide lactate as an energy substrate for neurons through a mechanism called "astrocyte-neuron lactate shuttle" (ANLS). Since neuronal activity changes dramatically during vigilance states, we hypothesized that the ANLS may be regulated during the sleep-wake cycle. To test this hypothesis we investigated the expression of genes associated with the ANLS specifcally in astrocytes following sleep deprivation. Astrocytes were purifed by fuorescence-activated cell sorting from transgenic mice expressing the green fuorescent protein (GFP) under the control of the human astrocytic GFAP-promoter. Design: 6-hour instrumental sleep deprivation (TSD). Setting: Animal sleep research laboratory. Participants: Young (P23-P27) FVB/N-Tg (GFAP-GFP) 14Mes/J (Tg) mice of both sexes and 7-8 week male Tg and FVB/Nj mice. Interventions: Basal sleep recordings and sleep deprivation achieved using a modifed cage where animals were gently forced to move. Measurements and Results: Since Tg and FVB/Nj mice displayed a similar sleep-wake pattern, we performed a TSD in young Tg mice. Total RNA was extracted from the GFP-positive and GFP-negative cells sorted from cerebral cortex. Quantitative RT-PCR analysis showed that levels of Glut1, a-2-Na/K pump, Glt1, and Ldha mRNAs were signifcantly increased following TSD in GFP-positive cells. In GFP-negative cells, a tendency to increase, although not signifcant, was observed for Ldha, Mct2, and α-3-Na/K pump mRNAs. Conclusions: This study shows that TSD induces the expression of genes associated with ANLS specifcally in astrocytes, underlying the important role of astrocytes in the maintenance of the neuro-metabolic coupling across the sleep-wake cycle.en
dc.description.sponsorshipThe authors warmly thank M. Garcia, G. Tapia, and S. Winkler of the Flow Cytometry Core Facility (Life Sciences Faculty, EPFL, Lausanne, Switzerland) for their technical expertise. This work was supported by a Swiss National Science Foundation grant (3100AO-108336/1) to Dr. Magistretti.en
dc.publisherAssociated Professional Sleep Societies (APSS)en
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3773194en
dc.subjectEnergy metabolismen
dc.subjectFow cytometryen
dc.subjectGliaen
dc.subjectGlucoseen
dc.subjectGlutamateen
dc.subjectTransgenic miceen
dc.titleGenes involved in the astrocyte-neuron lactate shuttle (ANLS) are specifcally regulated in cortical astrocytes following sleep deprivation in miceen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentBioscience Programen
dc.identifier.journalSLEEPen
dc.identifier.pmcidPMC3773194en
dc.contributor.institutionLNDC-BMI-SV, EPFL, Life Sciences Faculty, Swiss Federal Institute of Technology, Station 19, 1015 Lausanne, Switzerlanden
dc.contributor.institutionCenter for Psychiatric Neuroscience, Department of Psychiatry, CHUV, Prilly, Switzerlanden
kaust.authorMagistretti, Pierre J.en

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