Split photosystem protein, linear-mapping topology, and growth of structural complexity in the plastid genome of chromera velia

Handle URI:
http://hdl.handle.net/10754/562917
Title:
Split photosystem protein, linear-mapping topology, and growth of structural complexity in the plastid genome of chromera velia
Authors:
Janouškovec, Jan; Sobotka, Roman; Lai, Dehua; Flegontov, Pavel N.; Koník, Peter; Komenda, Josef; Ali, Shahjahan; Prášil, Ondřej; Pain, Arnab ( 0000-0002-1755-2819 ) ; Oborník, Miroslav; Lukeš, Juliuš; Keeling, Patrick J J.
Abstract:
The canonical photosynthetic plastid genomes consist of a single circular-mapping chromosome that encodes a highly conserved protein core, involved in photosynthesis and ATP generation. Here, we demonstrate that the plastid genome of the photosynthetic relative of apicomplexans, Chromera velia, departs from this view in several unique ways. Core photosynthesis proteins PsaA and AtpB have been broken into two fragments, which we show are independently transcribed, oligoU-tailed, translated, and assembled into functional photosystem I and ATP synthase complexes. Genome-wide transcription profiles support expression of many other highly modified proteins, including several that contain extensions amounting to hundreds of amino acids in length. Canonical gene clusters and operons have been fragmented and reshuffled into novel putative transcriptional units. Massive genomic coverage by paired-end reads, coupled with pulsed-field gel electrophoresis and polymerase chain reaction, consistently indicate that the C. velia plastid genome is linear-mapping, a unique state among all plastids. Abundant intragenomic duplication probably mediated by recombination can explain protein splits, extensions, and genome linearization and is perhaps the key driving force behind the many features that defy the conventional ways of plastid genome architecture and function. © The Author 2013.
KAUST Department:
Biosciences Core Lab; Computational Bioscience Research Center (CBRC); Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program; Core Labs; Pathogen Genomics Laboratory
Publisher:
Oxford University Press (OUP)
Journal:
Molecular Biology and Evolution
Issue Date:
22-Aug-2013
DOI:
10.1093/molbev/mst144
PubMed ID:
23974208
Type:
Article
ISSN:
07374038
Sponsors:
This work was supported by a grant from the Canadian Institutes of Health Research to P.J.K. (MOP-42517); by the Czech Science Foundation projects P506/12/1522 and P501/12/G055 to M.O.; by the Praemium Academiae award to J.L.; by Award IC/2010/09 by the King Abdullah University of Science and Technology (KAUST) to A. P., M.O., and J.L.; and by the project Algatech (CZ.1.05/2.1.00/03.0110) to R. S., J.K., and O.P.. P.J.K. and J.L. are Fellows of the Canadian Institute for Advanced Research. P.J.K. was supported by a Fellowship from the John Simon Guggenheim Foundation.
Appears in Collections:
Articles; Bioscience Program; Biosciences Core Lab; Computational Bioscience Research Center (CBRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorJanouškovec, Janen
dc.contributor.authorSobotka, Romanen
dc.contributor.authorLai, Dehuaen
dc.contributor.authorFlegontov, Pavel N.en
dc.contributor.authorKoník, Peteren
dc.contributor.authorKomenda, Josefen
dc.contributor.authorAli, Shahjahanen
dc.contributor.authorPrášil, Ondřejen
dc.contributor.authorPain, Arnaben
dc.contributor.authorOborník, Miroslaven
dc.contributor.authorLukeš, Juliušen
dc.contributor.authorKeeling, Patrick J J.en
dc.date.accessioned2015-08-03T11:15:24Zen
dc.date.available2015-08-03T11:15:24Zen
dc.date.issued2013-08-22en
dc.identifier.issn07374038en
dc.identifier.pmid23974208en
dc.identifier.doi10.1093/molbev/mst144en
dc.identifier.urihttp://hdl.handle.net/10754/562917en
dc.description.abstractThe canonical photosynthetic plastid genomes consist of a single circular-mapping chromosome that encodes a highly conserved protein core, involved in photosynthesis and ATP generation. Here, we demonstrate that the plastid genome of the photosynthetic relative of apicomplexans, Chromera velia, departs from this view in several unique ways. Core photosynthesis proteins PsaA and AtpB have been broken into two fragments, which we show are independently transcribed, oligoU-tailed, translated, and assembled into functional photosystem I and ATP synthase complexes. Genome-wide transcription profiles support expression of many other highly modified proteins, including several that contain extensions amounting to hundreds of amino acids in length. Canonical gene clusters and operons have been fragmented and reshuffled into novel putative transcriptional units. Massive genomic coverage by paired-end reads, coupled with pulsed-field gel electrophoresis and polymerase chain reaction, consistently indicate that the C. velia plastid genome is linear-mapping, a unique state among all plastids. Abundant intragenomic duplication probably mediated by recombination can explain protein splits, extensions, and genome linearization and is perhaps the key driving force behind the many features that defy the conventional ways of plastid genome architecture and function. © The Author 2013.en
dc.description.sponsorshipThis work was supported by a grant from the Canadian Institutes of Health Research to P.J.K. (MOP-42517); by the Czech Science Foundation projects P506/12/1522 and P501/12/G055 to M.O.; by the Praemium Academiae award to J.L.; by Award IC/2010/09 by the King Abdullah University of Science and Technology (KAUST) to A. P., M.O., and J.L.; and by the project Algatech (CZ.1.05/2.1.00/03.0110) to R. S., J.K., and O.P.. P.J.K. and J.L. are Fellows of the Canadian Institute for Advanced Research. P.J.K. was supported by a Fellowship from the John Simon Guggenheim Foundation.en
dc.publisherOxford University Press (OUP)en
dc.subjectChromera veliaen
dc.subjectLinear-mapping genomeen
dc.subjectPlastid genome evolutionen
dc.subjectSplit proteinen
dc.titleSplit photosystem protein, linear-mapping topology, and growth of structural complexity in the plastid genome of chromera veliaen
dc.typeArticleen
dc.contributor.departmentBiosciences Core Laben
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentBioscience Programen
dc.contributor.departmentCore Labsen
dc.contributor.departmentPathogen Genomics Laboratoryen
dc.identifier.journalMolecular Biology and Evolutionen
dc.contributor.institutionDepartment of Botany, University of British Columbia, Vancouver, BC, Canadaen
dc.contributor.institutionInstitute of Microbiology, Czech Academy of Sciences, Třeboň, Czech Republicen
dc.contributor.institutionFaculty of Science, University of South Bohemia, České Budějovice, Czech Republicen
dc.contributor.institutionBiology Centre, Institute of Parasitology, Czech Academy of Sciencess, České Budějovice, Czech Republicen
dc.contributor.institutionSchool of Life Sciences, Sun Yat-Sen University, Guangzhou, Chinaen
kaust.authorAli, Shahjahanen
kaust.authorPain, Arnaben

Related articles on PubMed

All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.