Molecular genetics and epigenetics of CACTA elements

Handle URI:
http://hdl.handle.net/10754/562915
Title:
Molecular genetics and epigenetics of CACTA elements
Authors:
Fedoroff, Nina V.
Abstract:
The CACTA transposons, so named for a highly conserved motif at element ends, comprise one of the most abundant superfamilies of Class 2 (cut-and-paste) plant transposons. CACTA transposons characteristically include subterminal sequences of several hundred nucleotides containing closely spaced direct and inverted repeats of a short, conserved sequence of 14-15 bp. The Supressor-mutator (Spm) transposon, identified and subjected to detailed genetic analysis by Barbara McClintock, remains the paradigmatic element of the CACTA family. The Spm transposon encodes two proteins required for transposition, the transposase (TnpD) and a regulatory protein (TnpA) that binds to the subterminal repeats. Spm expression is subject to both genetic and epigenetic regulation. The Spm-encoded TnpA serves as an activator of the epigenetically inactivated, methylated Spm, stimulating both transient and heritable activation of the transposon. TnpA also serves as a negative regulator of the demethylated active element promoter and is required, in addition to the TnpD, for transposition. © Springer Science+Business Media, New York 2013.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Publisher:
Humana Press
Journal:
Methods in Molecular Biology
Issue Date:
21-Aug-2013
DOI:
10.1007/978-1-62703-568-2-13
Type:
Article
ISSN:
10643745
ISBN:
9781627035675
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorFedoroff, Nina V.en
dc.date.accessioned2015-08-03T11:15:19Zen
dc.date.available2015-08-03T11:15:19Zen
dc.date.issued2013-08-21en
dc.identifier.isbn9781627035675en
dc.identifier.issn10643745en
dc.identifier.doi10.1007/978-1-62703-568-2-13en
dc.identifier.urihttp://hdl.handle.net/10754/562915en
dc.description.abstractThe CACTA transposons, so named for a highly conserved motif at element ends, comprise one of the most abundant superfamilies of Class 2 (cut-and-paste) plant transposons. CACTA transposons characteristically include subterminal sequences of several hundred nucleotides containing closely spaced direct and inverted repeats of a short, conserved sequence of 14-15 bp. The Supressor-mutator (Spm) transposon, identified and subjected to detailed genetic analysis by Barbara McClintock, remains the paradigmatic element of the CACTA family. The Spm transposon encodes two proteins required for transposition, the transposase (TnpD) and a regulatory protein (TnpA) that binds to the subterminal repeats. Spm expression is subject to both genetic and epigenetic regulation. The Spm-encoded TnpA serves as an activator of the epigenetically inactivated, methylated Spm, stimulating both transient and heritable activation of the transposon. TnpA also serves as a negative regulator of the demethylated active element promoter and is required, in addition to the TnpD, for transposition. © Springer Science+Business Media, New York 2013.en
dc.publisherHumana Pressen
dc.subjectCACTA elementsen
dc.subjectepigenetic regulationen
dc.subjectSpmen
dc.subjectSuppressor-mutatoren
dc.subjectTnpAen
dc.subjectTnpDen
dc.subjecttranspositionen
dc.subjecttransposonen
dc.titleMolecular genetics and epigenetics of CACTA elementsen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalMethods in Molecular Biologyen
dc.contributor.institutionHuck Institutes of the Life Sciences, Penn State University, University Park, PA, United Statesen
kaust.authorFedoroff, Nina V.en
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