Specificity and transcriptional activity of microbiota associated with low and high microbial abundance sponges from the Red Sea

Handle URI:
http://hdl.handle.net/10754/562913
Title:
Specificity and transcriptional activity of microbiota associated with low and high microbial abundance sponges from the Red Sea
Authors:
Moitinho-Silva, Lucas; Bayer, Kristina; Cannistraci, Carlo; Giles, Emily; Ryu, Tae Woo; Seridi, Loqmane; Ravasi, Timothy ( 0000-0002-9950-465X ) ; Hentschel, Ute T E
Abstract:
Marine sponges are generally classified as high microbial abundance (HMA) and low microbial abundance (LMA) species. Here, 16S rRNA amplicon sequencing was applied to investigate the diversity, specificity and transcriptional activity of microbes associated with an LMA sponge (Stylissa carteri), an HMA sponge (Xestospongia testudinaria) and sea water collected from the central Saudi Arabia coast of the Red Sea. Altogether, 887 068 denoised sequences were obtained, of which 806 661 sequences remained after quality control. This resulted in 1477 operational taxonomic units (OTUs) that were assigned to 27 microbial phyla. The microbial composition of S. carteri was more similar to that of sea water than to that of X. testudinaria, which is consistent with the observation that the sequence data set of S. carteri contained many more possibly sea water sequences (~24%) than the X. testudinaria data set (~6%). The most abundant OTUs were shared between all three sources (S. carteri, X. testudinaria, sea water), while rare OTUs were unique to any given source. Despite this high degree of overlap, each sponge species contained its own specific microbiota. The X. testudinaria-specific bacterial taxa were similar to those already described for this species. A set of S. carteri-specific bacterial taxa related to Proteobacteria and Nitrospira was identified, which are likely permanently associated with S. carteri. The transcriptional activity of sponge-associated microorganisms correlated well with their abundance. Quantitative PCR revealed the presence of Poribacteria, representing typical sponge symbionts, in both sponge species and in sea water; however, low transcriptional activity in sea water suggested that Poribacteria are not active outside the host context. © 2013 John Wiley & Sons Ltd.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Computational Bioscience Research Center (CBRC); Bioscience Program; Integrative Systems Biology Lab; Computer Science Program
Publisher:
Wiley-Blackwell
Journal:
Molecular Ecology
Issue Date:
20-Aug-2013
DOI:
10.1111/mec.12365
PubMed ID:
23957633
Type:
Article
ISSN:
09621083
Sponsors:
We thank the Coastal and Marine Resources Core Lab and the Biosciences Core Laboratory at KAUST. We acknowledge Susanne Schmitt for suggestion of PCR primers and Eva Reisberg for interesting discussions. The insightful comments of three anonymous reviewers have improved the quality of our manuscript. LM-S was supported by a grant of the German Excellence Initiative to the Graduate School of Life Sciences, University of Wuerzburg. Additional financial support was provided by KAUST baseline funds to TR.
Appears in Collections:
Articles; Bioscience Program; Computer Science Program; Computational Bioscience Research Center (CBRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorMoitinho-Silva, Lucasen
dc.contributor.authorBayer, Kristinaen
dc.contributor.authorCannistraci, Carloen
dc.contributor.authorGiles, Emilyen
dc.contributor.authorRyu, Tae Wooen
dc.contributor.authorSeridi, Loqmaneen
dc.contributor.authorRavasi, Timothyen
dc.contributor.authorHentschel, Ute T Een
dc.date.accessioned2015-08-03T11:15:14Zen
dc.date.available2015-08-03T11:15:14Zen
dc.date.issued2013-08-20en
dc.identifier.issn09621083en
dc.identifier.pmid23957633en
dc.identifier.doi10.1111/mec.12365en
dc.identifier.urihttp://hdl.handle.net/10754/562913en
dc.description.abstractMarine sponges are generally classified as high microbial abundance (HMA) and low microbial abundance (LMA) species. Here, 16S rRNA amplicon sequencing was applied to investigate the diversity, specificity and transcriptional activity of microbes associated with an LMA sponge (Stylissa carteri), an HMA sponge (Xestospongia testudinaria) and sea water collected from the central Saudi Arabia coast of the Red Sea. Altogether, 887 068 denoised sequences were obtained, of which 806 661 sequences remained after quality control. This resulted in 1477 operational taxonomic units (OTUs) that were assigned to 27 microbial phyla. The microbial composition of S. carteri was more similar to that of sea water than to that of X. testudinaria, which is consistent with the observation that the sequence data set of S. carteri contained many more possibly sea water sequences (~24%) than the X. testudinaria data set (~6%). The most abundant OTUs were shared between all three sources (S. carteri, X. testudinaria, sea water), while rare OTUs were unique to any given source. Despite this high degree of overlap, each sponge species contained its own specific microbiota. The X. testudinaria-specific bacterial taxa were similar to those already described for this species. A set of S. carteri-specific bacterial taxa related to Proteobacteria and Nitrospira was identified, which are likely permanently associated with S. carteri. The transcriptional activity of sponge-associated microorganisms correlated well with their abundance. Quantitative PCR revealed the presence of Poribacteria, representing typical sponge symbionts, in both sponge species and in sea water; however, low transcriptional activity in sea water suggested that Poribacteria are not active outside the host context. © 2013 John Wiley & Sons Ltd.en
dc.description.sponsorshipWe thank the Coastal and Marine Resources Core Lab and the Biosciences Core Laboratory at KAUST. We acknowledge Susanne Schmitt for suggestion of PCR primers and Eva Reisberg for interesting discussions. The insightful comments of three anonymous reviewers have improved the quality of our manuscript. LM-S was supported by a grant of the German Excellence Initiative to the Graduate School of Life Sciences, University of Wuerzburg. Additional financial support was provided by KAUST baseline funds to TR.en
dc.publisherWiley-Blackwellen
dc.subjectmarine spongesen
dc.subjectmicrobial diversityen
dc.subjectpyrosequencingen
dc.subjectsponge-specific microbesen
dc.subjectsymbiosisen
dc.subjecttranscriptional activityen
dc.titleSpecificity and transcriptional activity of microbiota associated with low and high microbial abundance sponges from the Red Seaen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.contributor.departmentBioscience Programen
dc.contributor.departmentIntegrative Systems Biology Laben
dc.contributor.departmentComputer Science Programen
dc.identifier.journalMolecular Ecologyen
dc.contributor.institutionDepartment of Botany II, Julius-von-Sachs Institute for Biological Sciences, University of Wuerzburg, Julius-von-Sachs Platz 3, 97082 Wuerzburg, Germanyen
kaust.authorCannistraci, Carloen
kaust.authorGiles, Emilyen
kaust.authorRyu, Tae Wooen
kaust.authorSeridi, Loqmaneen
kaust.authorRavasi, Timothyen

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