The Arabidopsis thaliana mutant air1 implicates SOS3 in the regulation of anthocyanins under salt stress

Handle URI:
http://hdl.handle.net/10754/562901
Title:
The Arabidopsis thaliana mutant air1 implicates SOS3 in the regulation of anthocyanins under salt stress
Authors:
Van Oosten, Michael James; Sharkhuu, Altanbadralt ( 0000-0002-4719-0265 ) ; Batelli, Giorgia; Bressan, Ray Anthony; Maggio, Albino
Abstract:
The accumulation of anthocyanins in plants exposed to salt stress has been largely documented. However, the functional link and regulatory components underlying the biosynthesis of these molecules during exposure to stress are largely unknown. In a screen of second site suppressors of the salt overly sensitive3-1 (sos3-1) mutant, we isolated the anthocyanin-impaired-response-1 (air1) mutant. air1 is unable to accumulate anthocyanins under salt stress, a key phenotype of sos3-1 under high NaCl levels (120 mM). The air1 mutant showed a defect in anthocyanin production in response to salt stress but not to other stresses such as high light, low phosphorous, high temperature or drought stress. This specificity indicated that air1 mutation did not affect anthocyanin biosynthesis but rather its regulation in response to salt stress. Analysis of this mutant revealed a T-DNA insertion at the first exon of an Arabidopsis thaliana gene encoding for a basic region-leucine zipper transcription factor. air1 mutants displayed higher survival rates compared to wild-type in oxidative stress conditions, and presented an altered expression of anthocyanin biosynthetic genes such as F3H, F3′H and LDOX in salt stress conditions. The results presented here indicate that AIR1 is involved in the regulation of various steps of the flavonoid and anthocyanin accumulation pathways and is itself regulated by the salt-stress response signalling machinery. The discovery and characterization of AIR1 opens avenues to dissect the connections between abiotic stress and accumulation of antioxidants in the form of flavonoids and anthocyanins. © 2013 Springer Science+Business Media Dordrecht.
KAUST Department:
Center for Desert Agriculture; Bioscience Program
Publisher:
Springer Nature
Journal:
Plant Molecular Biology
Issue Date:
8-Aug-2013
DOI:
10.1007/s11103-013-0099-z
PubMed ID:
23925404
Type:
Article
ISSN:
01674412
Sponsors:
The authors would like to thank Ana Rus for the original screen of sos3-1 suppressor mutants and Becky Fagan for her excellent administrative support. The authors would also like to thank the ABRC stock center for T-DNA mutant lines. A graduate fellowship for M.J.V. was provided by the Ross Fellowship program of Purdue University.
Appears in Collections:
Articles; Bioscience Program; Center for Desert Agriculture

Full metadata record

DC FieldValue Language
dc.contributor.authorVan Oosten, Michael Jamesen
dc.contributor.authorSharkhuu, Altanbadralten
dc.contributor.authorBatelli, Giorgiaen
dc.contributor.authorBressan, Ray Anthonyen
dc.contributor.authorMaggio, Albinoen
dc.date.accessioned2015-08-03T11:14:41Zen
dc.date.available2015-08-03T11:14:41Zen
dc.date.issued2013-08-08en
dc.identifier.issn01674412en
dc.identifier.pmid23925404en
dc.identifier.doi10.1007/s11103-013-0099-zen
dc.identifier.urihttp://hdl.handle.net/10754/562901en
dc.description.abstractThe accumulation of anthocyanins in plants exposed to salt stress has been largely documented. However, the functional link and regulatory components underlying the biosynthesis of these molecules during exposure to stress are largely unknown. In a screen of second site suppressors of the salt overly sensitive3-1 (sos3-1) mutant, we isolated the anthocyanin-impaired-response-1 (air1) mutant. air1 is unable to accumulate anthocyanins under salt stress, a key phenotype of sos3-1 under high NaCl levels (120 mM). The air1 mutant showed a defect in anthocyanin production in response to salt stress but not to other stresses such as high light, low phosphorous, high temperature or drought stress. This specificity indicated that air1 mutation did not affect anthocyanin biosynthesis but rather its regulation in response to salt stress. Analysis of this mutant revealed a T-DNA insertion at the first exon of an Arabidopsis thaliana gene encoding for a basic region-leucine zipper transcription factor. air1 mutants displayed higher survival rates compared to wild-type in oxidative stress conditions, and presented an altered expression of anthocyanin biosynthetic genes such as F3H, F3′H and LDOX in salt stress conditions. The results presented here indicate that AIR1 is involved in the regulation of various steps of the flavonoid and anthocyanin accumulation pathways and is itself regulated by the salt-stress response signalling machinery. The discovery and characterization of AIR1 opens avenues to dissect the connections between abiotic stress and accumulation of antioxidants in the form of flavonoids and anthocyanins. © 2013 Springer Science+Business Media Dordrecht.en
dc.description.sponsorshipThe authors would like to thank Ana Rus for the original screen of sos3-1 suppressor mutants and Becky Fagan for her excellent administrative support. The authors would also like to thank the ABRC stock center for T-DNA mutant lines. A graduate fellowship for M.J.V. was provided by the Ross Fellowship program of Purdue University.en
dc.publisherSpringer Natureen
dc.subjectAnthocyaninsen
dc.subjectArabidopsisen
dc.subjectFlavonoidsen
dc.subjectOxidative stressen
dc.subjectSalt stressen
dc.titleThe Arabidopsis thaliana mutant air1 implicates SOS3 in the regulation of anthocyanins under salt stressen
dc.typeArticleen
dc.contributor.departmentCenter for Desert Agricultureen
dc.contributor.departmentBioscience Programen
dc.identifier.journalPlant Molecular Biologyen
dc.contributor.institutionUniv Naples Federico II, Dept Agr, I-80055 Portici, Italyen
dc.contributor.institutionInst Plant Genet CNR IGV, Natl Res Council, I-80055 Portici, Italyen
dc.contributor.institutionPurdue Univ, Dept Hort & Landscape Architecture, W Lafayette, IN 47907 USAen
kaust.authorSharkhuu, Altanbadralten

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