Proteomic and metabolomic profiles of marine Vibrio sp. 010 in response to an antifoulant challenge

Handle URI:
http://hdl.handle.net/10754/562885
Title:
Proteomic and metabolomic profiles of marine Vibrio sp. 010 in response to an antifoulant challenge
Authors:
Chandramouli, Kondethimmanahalli ( 0000-0002-0104-1993 ) ; Dash, Swagatika; Zhang, Yu; Ravasi, Timothy ( 0000-0002-9950-465X ) ; Qian, Peiyuan
Abstract:
Vibrio spp. have the ability to form biofilms, which may contribute to the subsequent successful colonization by microfouling and macrofouling organisms. The effects of an antifouling compound, poly-ether B, on Vibrio sp. 010 were investigated using flow cytometry, proteomics, and metabolomics. A 2-D gel-based proteomic analysis was used to identify proteins responsive to poly-ether B treatment. The profiles of biofilm metabolites were analyzed by ultra-performance liquid chromatography-mass spectrometry. Poly-ether B caused a significant reduction in viability. The proteins affected by the treatment were related to nucleotide metabolism, the glyoxylate cycle, and stress responses. Metabolites such as tripeptides, fatty acids, and quorum-sensing molecules were regulated differentially. Down-regulation of proteins and metabolites potentially led to a loss in colonisation ability, thereby affecting the structure of the biofilm. These results suggest that the proteins and metabolites identified may serve as target molecules for potent antifouling compounds. © 2013 Copyright Taylor and Francis Group, LLC.
KAUST Department:
KAUST Global Collaborative Research Program; Applied Mathematics and Computational Science Program; Biological and Environmental Sciences and Engineering (BESE) Division; Integrative Systems Biology Lab; Bioscience Program; Computational Bioscience Research Center (CBRC)
Publisher:
Informa UK Limited
Journal:
Biofouling
Issue Date:
Aug-2013
DOI:
10.1080/08927014.2013.805209
PubMed ID:
23822634
Type:
Article
ISSN:
08927014
Sponsors:
The authors are grateful to Dr On On Lee, Ms Cherry Kwan, and Ms Emily Giles for proof-reading the manuscript and Ms Lisa Soo, Dr Jin Sun and Ms Crystal Lai for technical help with the experiments. This study was supported by a grant from China Ocean Mineral Resources Research and Development (DY125-15-T-02), a grant from Sanya Institute of Deep-Sea Science and Engineering (SIDSSE-201206), and an award (SA-C0040/UK-C0016) from the King Abdullah University of Science and Technology to PY Qian.
Appears in Collections:
Articles; Bioscience Program; Applied Mathematics and Computational Science Program; Computational Bioscience Research Center (CBRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorChandramouli, Kondethimmanahallien
dc.contributor.authorDash, Swagatikaen
dc.contributor.authorZhang, Yuen
dc.contributor.authorRavasi, Timothyen
dc.contributor.authorQian, Peiyuanen
dc.date.accessioned2015-08-03T11:14:06Zen
dc.date.available2015-08-03T11:14:06Zen
dc.date.issued2013-08en
dc.identifier.issn08927014en
dc.identifier.pmid23822634en
dc.identifier.doi10.1080/08927014.2013.805209en
dc.identifier.urihttp://hdl.handle.net/10754/562885en
dc.description.abstractVibrio spp. have the ability to form biofilms, which may contribute to the subsequent successful colonization by microfouling and macrofouling organisms. The effects of an antifouling compound, poly-ether B, on Vibrio sp. 010 were investigated using flow cytometry, proteomics, and metabolomics. A 2-D gel-based proteomic analysis was used to identify proteins responsive to poly-ether B treatment. The profiles of biofilm metabolites were analyzed by ultra-performance liquid chromatography-mass spectrometry. Poly-ether B caused a significant reduction in viability. The proteins affected by the treatment were related to nucleotide metabolism, the glyoxylate cycle, and stress responses. Metabolites such as tripeptides, fatty acids, and quorum-sensing molecules were regulated differentially. Down-regulation of proteins and metabolites potentially led to a loss in colonisation ability, thereby affecting the structure of the biofilm. These results suggest that the proteins and metabolites identified may serve as target molecules for potent antifouling compounds. © 2013 Copyright Taylor and Francis Group, LLC.en
dc.description.sponsorshipThe authors are grateful to Dr On On Lee, Ms Cherry Kwan, and Ms Emily Giles for proof-reading the manuscript and Ms Lisa Soo, Dr Jin Sun and Ms Crystal Lai for technical help with the experiments. This study was supported by a grant from China Ocean Mineral Resources Research and Development (DY125-15-T-02), a grant from Sanya Institute of Deep-Sea Science and Engineering (SIDSSE-201206), and an award (SA-C0040/UK-C0016) from the King Abdullah University of Science and Technology to PY Qian.en
dc.publisherInforma UK Limiteden
dc.subjectbiofilmen
dc.subjectmetabolomeen
dc.subjectpoly-ether Ben
dc.subjectproteomeen
dc.subjectVibrio sp.en
dc.titleProteomic and metabolomic profiles of marine Vibrio sp. 010 in response to an antifoulant challengeen
dc.typeArticleen
dc.contributor.departmentKAUST Global Collaborative Research Programen
dc.contributor.departmentApplied Mathematics and Computational Science Programen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentIntegrative Systems Biology Laben
dc.contributor.departmentBioscience Programen
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.identifier.journalBiofoulingen
dc.contributor.institutionShenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, College of Life Science, Shenzhen University, Shenzhen, Chinaen
kaust.authorChandramouli, Kondethimmanahallien
kaust.authorRavasi, Timothyen

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