A bi-functional xyloglucan galactosyltransferase is an indispensable salt stress tolerance determinant in arabidop

Handle URI:
http://hdl.handle.net/10754/562850
Title:
A bi-functional xyloglucan galactosyltransferase is an indispensable salt stress tolerance determinant in arabidop
Authors:
Li, Wenbo; Guan, Qingmei; Wang, Zhenyu; Wang, Yingdian; Zhu, Jianhua
Abstract:
Salinity is an abiotic stress that substantially limits crop production worldwide. To identify salt stress tolerance determinants, we screened for Arabidopsis mutants that are hypersensitive to salt stress and designated these mutants as short root in salt medium (rsa). One of these mutants, rsa3-1, is hypersensitive to NaCl and LiCl but not to CsCl or to general osmotic stress. Reactive oxygen species (ROS) over-accumulate in rsa3-1 plants under salt stress. Gene expression profiling with Affymetrix microarray analysis revealed that RSA3 controls expression of many genes including genes encoding proteins for ROS detoxification under salt stress. Map-based cloning showed that RSA3 encodes a xyloglucan galactosyltransferase, which is allelic to a gene previously named MUR3/KAM1. The RSA3/ MUR3/KAM1-encoded xylogluscan galactosyltransferase regulates actin microfilament organization (and thereby contributes to endomembrane distribution) and is also involved in cell wall biosynthesis. In rsa3-1, actin cannot assemble and form bundles as it does in the wild-type but instead aggregates in the cytoplasm. Furthermore, addition of phalloidin, which prevents actin depolymerization, can rescue salt hypersensitivity of rsa3-1. Together, these results suggest that RSA3/MUR3/KAM1 along with other cell wall-associated proteins plays a critical role in salt stress tolerance by maintaining the proper organization of actin microfilaments in order to minimize damage caused by excessive ROS. © 2013 The Author.
KAUST Department:
Center for Desert Agriculture; Biological and Environmental Sciences and Engineering (BESE) Division
Publisher:
Elsevier BV
Journal:
Molecular Plant
Issue Date:
Jul-2013
DOI:
10.1093/mp/sst062
PubMed ID:
23571490
Type:
Article
ISSN:
16742052
Sponsors:
This work was supported by National Science Foundation (NSF) grants IOS0919745 and MCB0950242 to J.Z. and by NSF grant DBI0922650.
Appears in Collections:
Articles; Center for Desert Agriculture; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorLi, Wenboen
dc.contributor.authorGuan, Qingmeien
dc.contributor.authorWang, Zhenyuen
dc.contributor.authorWang, Yingdianen
dc.contributor.authorZhu, Jianhuaen
dc.date.accessioned2015-08-03T11:12:43Zen
dc.date.available2015-08-03T11:12:43Zen
dc.date.issued2013-07en
dc.identifier.issn16742052en
dc.identifier.pmid23571490en
dc.identifier.doi10.1093/mp/sst062en
dc.identifier.urihttp://hdl.handle.net/10754/562850en
dc.description.abstractSalinity is an abiotic stress that substantially limits crop production worldwide. To identify salt stress tolerance determinants, we screened for Arabidopsis mutants that are hypersensitive to salt stress and designated these mutants as short root in salt medium (rsa). One of these mutants, rsa3-1, is hypersensitive to NaCl and LiCl but not to CsCl or to general osmotic stress. Reactive oxygen species (ROS) over-accumulate in rsa3-1 plants under salt stress. Gene expression profiling with Affymetrix microarray analysis revealed that RSA3 controls expression of many genes including genes encoding proteins for ROS detoxification under salt stress. Map-based cloning showed that RSA3 encodes a xyloglucan galactosyltransferase, which is allelic to a gene previously named MUR3/KAM1. The RSA3/ MUR3/KAM1-encoded xylogluscan galactosyltransferase regulates actin microfilament organization (and thereby contributes to endomembrane distribution) and is also involved in cell wall biosynthesis. In rsa3-1, actin cannot assemble and form bundles as it does in the wild-type but instead aggregates in the cytoplasm. Furthermore, addition of phalloidin, which prevents actin depolymerization, can rescue salt hypersensitivity of rsa3-1. Together, these results suggest that RSA3/MUR3/KAM1 along with other cell wall-associated proteins plays a critical role in salt stress tolerance by maintaining the proper organization of actin microfilaments in order to minimize damage caused by excessive ROS. © 2013 The Author.en
dc.description.sponsorshipThis work was supported by National Science Foundation (NSF) grants IOS0919745 and MCB0950242 to J.Z. and by NSF grant DBI0922650.en
dc.publisherElsevier BVen
dc.subjectactin microfilamentsen
dc.subjectendomembranesen
dc.subjectRSA3en
dc.subjectSalt stress toleranceen
dc.subjectxyloglucan galactosyltransferaseen
dc.titleA bi-functional xyloglucan galactosyltransferase is an indispensable salt stress tolerance determinant in arabidopen
dc.typeArticleen
dc.contributor.departmentCenter for Desert Agricultureen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalMolecular Planten
dc.contributor.institutionCollege of Life Sciences, Beijing Normal University, Beijing, 100875, Chinaen
dc.contributor.institutionDepartment of Plant Science and Landscape Architecture, University of Maryland, College Park, MD 20742, United Statesen
kaust.authorWang, Zhenyuen
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