Molecular cloning, sequencing and structural studies of granulocyte-macrophage colony-stimulating factor (GM-CSF) from Indian water buffalo (Bubalus bubalis)

Handle URI:
http://hdl.handle.net/10754/562823
Title:
Molecular cloning, sequencing and structural studies of granulocyte-macrophage colony-stimulating factor (GM-CSF) from Indian water buffalo (Bubalus bubalis)
Authors:
Sugumar, Thennarasu; Ganesan, Pugalenthi; Harishankar, Murugesan; Dhinakar Raj, Gopal
Abstract:
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine that is essential for growth and development of progenitors of granulocytes and monocytes/macrophages. In this study, we report molecular cloning, sequencing and characterization of GM-CSF from Indian water buffalo, Bubalus bubalis. In addition, we performed sequence and structural analysis for buffalo GM-CSF. Buffalo GM-CSF has been compared with 17 mammalian GM-CSFs using multiple sequence alignment and phylogenetic tree. Three-dimensional model for buffalo GM-CSF and human receptor complex was built using homology modelling to study cross-reactivity between two species. Detailed analysis was performed to study GM-CSF interface and various interactions at the interface. © 2013 John Wiley & Sons Ltd.
KAUST Department:
Biosciences Core Lab; Core Labs
Publisher:
Wiley-Blackwell
Journal:
International Journal of Immunogenetics
Issue Date:
25-Jun-2013
DOI:
10.1111/iji.12074
PubMed ID:
23800159
Type:
Article
ISSN:
17443121
Sponsors:
The authors thank the Department of Biotechnology (DBT), Government of India, for the financial assistance to this Project. ST and MHS were SRFs who worked in this project. GP carried out the bioinformatics analysis.
Appears in Collections:
Articles; Biosciences Core Lab

Full metadata record

DC FieldValue Language
dc.contributor.authorSugumar, Thennarasuen
dc.contributor.authorGanesan, Pugalenthien
dc.contributor.authorHarishankar, Murugesanen
dc.contributor.authorDhinakar Raj, Gopalen
dc.date.accessioned2015-08-03T11:11:38Zen
dc.date.available2015-08-03T11:11:38Zen
dc.date.issued2013-06-25en
dc.identifier.issn17443121en
dc.identifier.pmid23800159en
dc.identifier.doi10.1111/iji.12074en
dc.identifier.urihttp://hdl.handle.net/10754/562823en
dc.description.abstractGranulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine that is essential for growth and development of progenitors of granulocytes and monocytes/macrophages. In this study, we report molecular cloning, sequencing and characterization of GM-CSF from Indian water buffalo, Bubalus bubalis. In addition, we performed sequence and structural analysis for buffalo GM-CSF. Buffalo GM-CSF has been compared with 17 mammalian GM-CSFs using multiple sequence alignment and phylogenetic tree. Three-dimensional model for buffalo GM-CSF and human receptor complex was built using homology modelling to study cross-reactivity between two species. Detailed analysis was performed to study GM-CSF interface and various interactions at the interface. © 2013 John Wiley & Sons Ltd.en
dc.description.sponsorshipThe authors thank the Department of Biotechnology (DBT), Government of India, for the financial assistance to this Project. ST and MHS were SRFs who worked in this project. GP carried out the bioinformatics analysis.en
dc.publisherWiley-Blackwellen
dc.titleMolecular cloning, sequencing and structural studies of granulocyte-macrophage colony-stimulating factor (GM-CSF) from Indian water buffalo (Bubalus bubalis)en
dc.typeArticleen
dc.contributor.departmentBiosciences Core Laben
dc.contributor.departmentCore Labsen
dc.identifier.journalInternational Journal of Immunogeneticsen
dc.contributor.institutionDepartment of Immunology, Tuberculosis Research Centre, Indian Council of Medical Research, Chennai, Indiaen
dc.contributor.institutionDepartment of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Indiaen
kaust.authorSugumar, Thennarasuen
kaust.authorGanesan, Pugalenthien

Related articles on PubMed

All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.