Characterization of bacterial and archaeal communities in air-cathode microbial fuel cells, open circuit and sealed-off reactors

Handle URI:
http://hdl.handle.net/10754/562815
Title:
Characterization of bacterial and archaeal communities in air-cathode microbial fuel cells, open circuit and sealed-off reactors
Authors:
Chehab, Noura A. ( 0000-0002-5197-9517 ) ; Li, Dong; Amy, Gary L.; Logan, Bruce E.; Saikaly, Pascal ( 0000-0001-7678-3986 )
Abstract:
A large percentage of organic fuel consumed in a microbial fuel cell (MFC) is lost as a result of oxygen transfer through the cathode. In order to understand how this oxygen transfer affects the microbial community structure, reactors were operated in duplicate using three configurations: closed circuit (CC; with current generation), open circuit (OC; no current generation), and sealed off cathodes (SO; no current, with a solid plate placed across the cathode). Most (98 %) of the chemical oxygen demand (COD) was removed during power production in the CC reactor (maximum of 640 ± 10 mW/m 2), with a low percent of substrate converted to current (coulombic efficiency of 26.5 ± 2.1 %). Sealing the cathode reduced COD removal to 7 %, but with an open cathode, there was nearly as much COD removal by the OC reactor (94.5 %) as the CC reactor. Oxygen transfer into the reactor substantially affected the composition of the microbial communities. Based on analysis of the biofilms using 16S rRNA gene pyrosequencing, microbes most similar to Geobacter were predominant on the anodes in the CC MFC (72 % of sequences), but the most abundant bacteria were Azoarcus (42 to 47 %) in the OC reactor, and Dechloromonas (17 %) in the SO reactor. Hydrogenotrophic methanogens were most predominant, with sequences most similar to Methanobacterium in the CC and SO reactor, and Methanocorpusculum in the OC reactors. These results show that oxygen leakage through the cathode substantially alters the bacterial anode communities, and that hydrogenotrophic methanogens predominate despite high concentrations of acetate. The predominant methanogens in the CC reactor most closely resembled those in the SO reactor, demonstrating that oxygen leakage alters methanogenic as well as general bacterial communities. © 2013 Springer-Verlag Berlin Heidelberg.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Water Desalination and Reuse Research Center (WDRC); Environmental Science and Engineering Program; Water Desalination & Reuse Research Cntr; Environmental Biotechnology Research Group
Publisher:
Springer Nature
Journal:
Applied Microbiology and Biotechnology
Issue Date:
18-Jun-2013
DOI:
10.1007/s00253-013-5025-4
PubMed ID:
23775270
Type:
Article
ISSN:
01757598
Sponsors:
This work was sponsored by a PhD fellowship, a Global Research Partnership-Collaborative Fellows award (GRP-CF-2011-14-S), KAUST Award KUS-I1-003-13 (Bruce E. Logan) and discretionary investigator funds (Pascal E. Saikaly) at King Abdullah University of Science and Technology (KAUST). Special thanks are extended to Hari Ananda Rao for PCR assistance and Mohammed Alarawi and Dr. Shahjahan Ali of the Bioscience Core Laboratory at KAUST for 454 pyrosequencing.
Appears in Collections:
Articles; Environmental Science and Engineering Program; Water Desalination and Reuse Research Center (WDRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorChehab, Noura A.en
dc.contributor.authorLi, Dongen
dc.contributor.authorAmy, Gary L.en
dc.contributor.authorLogan, Bruce E.en
dc.contributor.authorSaikaly, Pascalen
dc.date.accessioned2015-08-03T11:11:20Zen
dc.date.available2015-08-03T11:11:20Zen
dc.date.issued2013-06-18en
dc.identifier.issn01757598en
dc.identifier.pmid23775270en
dc.identifier.doi10.1007/s00253-013-5025-4en
dc.identifier.urihttp://hdl.handle.net/10754/562815en
dc.description.abstractA large percentage of organic fuel consumed in a microbial fuel cell (MFC) is lost as a result of oxygen transfer through the cathode. In order to understand how this oxygen transfer affects the microbial community structure, reactors were operated in duplicate using three configurations: closed circuit (CC; with current generation), open circuit (OC; no current generation), and sealed off cathodes (SO; no current, with a solid plate placed across the cathode). Most (98 %) of the chemical oxygen demand (COD) was removed during power production in the CC reactor (maximum of 640 ± 10 mW/m 2), with a low percent of substrate converted to current (coulombic efficiency of 26.5 ± 2.1 %). Sealing the cathode reduced COD removal to 7 %, but with an open cathode, there was nearly as much COD removal by the OC reactor (94.5 %) as the CC reactor. Oxygen transfer into the reactor substantially affected the composition of the microbial communities. Based on analysis of the biofilms using 16S rRNA gene pyrosequencing, microbes most similar to Geobacter were predominant on the anodes in the CC MFC (72 % of sequences), but the most abundant bacteria were Azoarcus (42 to 47 %) in the OC reactor, and Dechloromonas (17 %) in the SO reactor. Hydrogenotrophic methanogens were most predominant, with sequences most similar to Methanobacterium in the CC and SO reactor, and Methanocorpusculum in the OC reactors. These results show that oxygen leakage through the cathode substantially alters the bacterial anode communities, and that hydrogenotrophic methanogens predominate despite high concentrations of acetate. The predominant methanogens in the CC reactor most closely resembled those in the SO reactor, demonstrating that oxygen leakage alters methanogenic as well as general bacterial communities. © 2013 Springer-Verlag Berlin Heidelberg.en
dc.description.sponsorshipThis work was sponsored by a PhD fellowship, a Global Research Partnership-Collaborative Fellows award (GRP-CF-2011-14-S), KAUST Award KUS-I1-003-13 (Bruce E. Logan) and discretionary investigator funds (Pascal E. Saikaly) at King Abdullah University of Science and Technology (KAUST). Special thanks are extended to Hari Ananda Rao for PCR assistance and Mohammed Alarawi and Dr. Shahjahan Ali of the Bioscience Core Laboratory at KAUST for 454 pyrosequencing.en
dc.publisherSpringer Natureen
dc.subject16S rRNA gene pyrosequencingen
dc.subjectAir-cathodeen
dc.subjectMicrobial communityen
dc.subjectOxygen intrusionen
dc.titleCharacterization of bacterial and archaeal communities in air-cathode microbial fuel cells, open circuit and sealed-off reactorsen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)en
dc.contributor.departmentEnvironmental Science and Engineering Programen
dc.contributor.departmentWater Desalination & Reuse Research Cntren
dc.contributor.departmentEnvironmental Biotechnology Research Groupen
dc.identifier.journalApplied Microbiology and Biotechnologyen
dc.contributor.institutionDepartment of Civil and Environmental Engineering, Pennsylvania State University, University Park, PA 16802, United Statesen
kaust.authorChehab, Noura A.en
kaust.authorLi, Dongen
kaust.authorAmy, Gary L.en
kaust.authorSaikaly, Pascalen

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