Arabidopsis cysteine-rich receptor-like kinase 45 functions in the responses to abscisic acid and abiotic stresses

Handle URI:
http://hdl.handle.net/10754/562786
Title:
Arabidopsis cysteine-rich receptor-like kinase 45 functions in the responses to abscisic acid and abiotic stresses
Authors:
Zhang, Xiujuan; Yang, Guanyu; Shi, Rui; Han, Xiaomin; Qi, Liwang; Wang, Ruigang; Xiong, Liming ( 0000-0001-8099-0806 ) ; Li, Guojing
Abstract:
The phytohormone abscisic acid (ABA) regulates seed germination, plant growth and development, and response to abiotic stresses such as drought and salt stresses. Receptor-like kinases are well known signaling components that mediate plant responses to developmental and environmental stimuli. Here, we characterized the biological function of an ABA and stress-inducible cysteine-rich receptor-like protein kinase, CRK45, in ABA signaling in Arabidopsis thaliana. The crk45 mutant was less sensitive to ABA than the wild type during seed germination and early seedling development, whereas CRK45 overexpression plants were more sensitive to ABA compared to the wild type. Furthermore, overexpression of CRK45 led to hypersensitivity to salt and glucose inhibition of seed germination, whereas the crk45 mutant showed the opposite phenotypes. In addition, CRK45 overexpression plants had enhanced tolerance to drought. Gene expression analyses revealed that the expression of representative stress-responsive genes was significantly enhanced in CRK45 overexpression plants in response to salt stress. ABA biosynthetic genes such as NCED3,. 22NCED3, 9-Cis-Epoxycarotenoid Dioxygenase 3.NCED5,. 33NCED5, 9-Cis-Epoxycarotenoid Dioxygenase 5.ABA2,. 44ABA2, Abscisic Acid Deficient 2. and AAO355AAO3, Abscisic Aldehyde Oxidase 3. were also constitutively elevated in the CRK45 overexpression plants. We concluded that CRK45 plays an important role in ABA signaling that regulates Arabidopsis seeds germination, early seedling development and abiotic stresses response, by positively regulating ABA responses in these processes. © 2013 Elsevier Masson SAS.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Center for Desert Agriculture; Bioscience Program; Plant Stress Genomics Research Lab
Publisher:
Elsevier BV
Journal:
Plant Physiology and Biochemistry
Issue Date:
Jun-2013
DOI:
10.1016/j.plaphy.2013.03.013
PubMed ID:
23583936
Type:
Article
ISSN:
09819428
Sponsors:
We thank Dr. Shuqun Zhang for critical reading of the manuscript, and Kathleen M. Buckley, M.S. for editing assistance. This work was supported by the grants from National Natural Science Foundation and Ministry of Education of China (grant nos. 30860029 and NCET-11-1020 to R.W.), and from Ministry of Science and Technology of China and Inner Mongolia Agricultural University (grant nos. 2011AA100203 and NDPYTD2010-3 to G.L.).
Appears in Collections:
Articles; Bioscience Program; Center for Desert Agriculture; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorZhang, Xiujuanen
dc.contributor.authorYang, Guanyuen
dc.contributor.authorShi, Ruien
dc.contributor.authorHan, Xiaominen
dc.contributor.authorQi, Liwangen
dc.contributor.authorWang, Ruigangen
dc.contributor.authorXiong, Limingen
dc.contributor.authorLi, Guojingen
dc.date.accessioned2015-08-03T11:05:41Zen
dc.date.available2015-08-03T11:05:41Zen
dc.date.issued2013-06en
dc.identifier.issn09819428en
dc.identifier.pmid23583936en
dc.identifier.doi10.1016/j.plaphy.2013.03.013en
dc.identifier.urihttp://hdl.handle.net/10754/562786en
dc.description.abstractThe phytohormone abscisic acid (ABA) regulates seed germination, plant growth and development, and response to abiotic stresses such as drought and salt stresses. Receptor-like kinases are well known signaling components that mediate plant responses to developmental and environmental stimuli. Here, we characterized the biological function of an ABA and stress-inducible cysteine-rich receptor-like protein kinase, CRK45, in ABA signaling in Arabidopsis thaliana. The crk45 mutant was less sensitive to ABA than the wild type during seed germination and early seedling development, whereas CRK45 overexpression plants were more sensitive to ABA compared to the wild type. Furthermore, overexpression of CRK45 led to hypersensitivity to salt and glucose inhibition of seed germination, whereas the crk45 mutant showed the opposite phenotypes. In addition, CRK45 overexpression plants had enhanced tolerance to drought. Gene expression analyses revealed that the expression of representative stress-responsive genes was significantly enhanced in CRK45 overexpression plants in response to salt stress. ABA biosynthetic genes such as NCED3,. 22NCED3, 9-Cis-Epoxycarotenoid Dioxygenase 3.NCED5,. 33NCED5, 9-Cis-Epoxycarotenoid Dioxygenase 5.ABA2,. 44ABA2, Abscisic Acid Deficient 2. and AAO355AAO3, Abscisic Aldehyde Oxidase 3. were also constitutively elevated in the CRK45 overexpression plants. We concluded that CRK45 plays an important role in ABA signaling that regulates Arabidopsis seeds germination, early seedling development and abiotic stresses response, by positively regulating ABA responses in these processes. © 2013 Elsevier Masson SAS.en
dc.description.sponsorshipWe thank Dr. Shuqun Zhang for critical reading of the manuscript, and Kathleen M. Buckley, M.S. for editing assistance. This work was supported by the grants from National Natural Science Foundation and Ministry of Education of China (grant nos. 30860029 and NCET-11-1020 to R.W.), and from Ministry of Science and Technology of China and Inner Mongolia Agricultural University (grant nos. 2011AA100203 and NDPYTD2010-3 to G.L.).en
dc.publisherElsevier BVen
dc.subjectAbscisic aciden
dc.subjectArabidopsis thalianaen
dc.subjectCRK45en
dc.subjectDroughten
dc.subjectEarly seedling establishmenten
dc.subjectGerminationen
dc.subjectSalten
dc.titleArabidopsis cysteine-rich receptor-like kinase 45 functions in the responses to abscisic acid and abiotic stressesen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentCenter for Desert Agricultureen
dc.contributor.departmentBioscience Programen
dc.contributor.departmentPlant Stress Genomics Research Laben
dc.identifier.journalPlant Physiology and Biochemistryen
dc.contributor.institutionCollege of Life Sciences, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, Chinaen
dc.contributor.institutionThe Research Institute of Forestry, The Chinese Academy of Forestry, Beijing 100091, Chinaen
kaust.authorXiong, Limingen

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