Light harvesting complexes of Chromera velia, photosynthetic relative of apicomplexan parasites

Handle URI:
http://hdl.handle.net/10754/562781
Title:
Light harvesting complexes of Chromera velia, photosynthetic relative of apicomplexan parasites
Authors:
Tichý, Josef; Gardian, Zdenko; Bína, David; Koník, Peter; Litvín, Radek V.; Herbstová, Miroslava; Pain, Arnab ( 0000-0002-1755-2819 ) ; Vácha, František
Abstract:
The structure and composition of the light harvesting complexes from the unicellular alga Chromera velia were studied by means of optical spectroscopy, biochemical and electron microscopy methods. Two different types of antennae systems were identified. One exhibited a molecular weight (18-19 kDa) similar to FCP (fucoxanthin chlorophyll protein) complexes from diatoms, however, single particle analysis and circular dichroism spectroscopy indicated similarity of this structure to the recently characterized XLH antenna of xanthophytes. In light of these data we denote this antenna complex CLH, for "Chromera Light Harvesting" complex. The other system was identified as the photosystem I with bound Light Harvesting Complexes (PSI-LHCr) related to the red algae LHCI antennae. The result of this study is the finding that C. velia, when grown in natural light conditions, possesses light harvesting antennae typically found in two different, evolutionary distant, groups of photosynthetic organisms. © 2013 Elsevier B.V. All rights reserved.
KAUST Department:
Computational Bioscience Research Center (CBRC); Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program; Pathogen Genomics Laboratory
Publisher:
Elsevier BV
Journal:
Biochimica et Biophysica Acta (BBA) - Bioenergetics
Issue Date:
Jun-2013
DOI:
10.1016/j.bbabio.2013.02.002
PubMed ID:
23428396
Type:
Article
ISSN:
00052728
Sponsors:
This research was supported by the GAAV 32-IAA601410907 and GAJU 134/2010/P with institutional support RVO:60077344. Research on C. velia genomics is supported by an Award IC/2010/09 made by the King Abdullah University of Science and Technology (KAUST) to A. Pain. The authors are greatly indebted to the team of J. Lukes, R. Sobotka and P. Keeling, for giving access to their data concerning the split of the photosystem I reaction center protein prior to publication. Thanks are extended to I. Hunalova and F. Matousek for technical assistance.
Appears in Collections:
Articles; Bioscience Program; Computational Bioscience Research Center (CBRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorTichý, Josefen
dc.contributor.authorGardian, Zdenkoen
dc.contributor.authorBína, Daviden
dc.contributor.authorKoník, Peteren
dc.contributor.authorLitvín, Radek V.en
dc.contributor.authorHerbstová, Miroslavaen
dc.contributor.authorPain, Arnaben
dc.contributor.authorVácha, Františeken
dc.date.accessioned2015-08-03T11:05:29Zen
dc.date.available2015-08-03T11:05:29Zen
dc.date.issued2013-06en
dc.identifier.issn00052728en
dc.identifier.pmid23428396en
dc.identifier.doi10.1016/j.bbabio.2013.02.002en
dc.identifier.urihttp://hdl.handle.net/10754/562781en
dc.description.abstractThe structure and composition of the light harvesting complexes from the unicellular alga Chromera velia were studied by means of optical spectroscopy, biochemical and electron microscopy methods. Two different types of antennae systems were identified. One exhibited a molecular weight (18-19 kDa) similar to FCP (fucoxanthin chlorophyll protein) complexes from diatoms, however, single particle analysis and circular dichroism spectroscopy indicated similarity of this structure to the recently characterized XLH antenna of xanthophytes. In light of these data we denote this antenna complex CLH, for "Chromera Light Harvesting" complex. The other system was identified as the photosystem I with bound Light Harvesting Complexes (PSI-LHCr) related to the red algae LHCI antennae. The result of this study is the finding that C. velia, when grown in natural light conditions, possesses light harvesting antennae typically found in two different, evolutionary distant, groups of photosynthetic organisms. © 2013 Elsevier B.V. All rights reserved.en
dc.description.sponsorshipThis research was supported by the GAAV 32-IAA601410907 and GAJU 134/2010/P with institutional support RVO:60077344. Research on C. velia genomics is supported by an Award IC/2010/09 made by the King Abdullah University of Science and Technology (KAUST) to A. Pain. The authors are greatly indebted to the team of J. Lukes, R. Sobotka and P. Keeling, for giving access to their data concerning the split of the photosystem I reaction center protein prior to publication. Thanks are extended to I. Hunalova and F. Matousek for technical assistance.en
dc.publisherElsevier BVen
dc.subjectChromera veliaen
dc.subjectCircular dichroismen
dc.subjectElectron microscopyen
dc.subjectFCPen
dc.subjectLHCren
dc.subjectPhotosystem Ien
dc.titleLight harvesting complexes of Chromera velia, photosynthetic relative of apicomplexan parasitesen
dc.typeArticleen
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentBioscience Programen
dc.contributor.departmentPathogen Genomics Laboratoryen
dc.identifier.journalBiochimica et Biophysica Acta (BBA) - Bioenergeticsen
dc.contributor.institutionFaculty of Science, University of South Bohemia, Branisovska 31, 37005 Ceske Budejovice, Czech Republicen
dc.contributor.institutionInstitute of Plant Molecular Biology, Biology Centre ASCR, Branisovska 31, 37005 Ceske Budejovice, Czech Republicen
kaust.authorPain, Arnaben

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