Maturation and demise of human primary monocytes by carbon nanotubes

Handle URI:
http://hdl.handle.net/10754/562766
Title:
Maturation and demise of human primary monocytes by carbon nanotubes
Authors:
De Nicola, Milena D.; Mirabile Gattia, Daniele; Traversa, Enrico ( 0000-0001-6336-941X ) ; Ghibelli, Lina
Abstract:
The possibility of exploiting carbon nanotubes (CNT) in biomedical practices requires thorough analysis of the chemical or bulk effects they may exert on the immune system, the complex network that recognizes and eliminates foreign particles. In particular, the phagocytosing ability of cells belonging to the monocyte/macrophage lineage may render these immune cells an ideal toxicological target of pristine CNT, which may form aggregates of size exceeding monocyte/macrophage phagocytosing plasticity. To shed light on this issue, we analyzed the effects that pristine multi-walled CNT (MWCNT) without metal or biological impurities exert on survival and activation of freshly explanted human peripheral blood monocytes, analyzing in parallel the non-phagocytosing lymphocytes, and using graphite as control carbon material. MWCNT (diameter 10-50 nm, length up to 10 μm) exert two different toxic effects on mononuclear leukocytes: a minor apoptogenic effect (on lymphocytes > monocytes), and a major, apoptosis-independent effect that exclusively and deeply affect monocyte homeostasis. Analysis of monocyte number, adhesion, redox equilibrium, and the differentiation markers CD14 and CD11b reveals that MWCNT cause the selective disappearance of phagocytosis-competent monocytes by mechanisms related to the presence of large nanoparticle aggregates, suggesting phenomena of bulk toxicity possibly consisting of frustrated phagocytosis. At the same time, MWCNT stimulate adhesion of the phagocytosis-incompetent monocytes, and their differentiation toward a peculiar maturation asset. These observations point out novel mechanisms of CNT toxicity, renewing concerns that they may impair the innate immune system deranging the inflammatory responses. © 2013 Springer Science+Business Media Dordrecht.
KAUST Department:
Physical Sciences and Engineering (PSE) Division; Materials Science and Engineering Program; Solar and Photovoltaic Engineering Research Center (SPERC); Materials for Energy Conversion and Storage (MECS) Lab
Publisher:
Springer Verlag
Journal:
Journal of Nanoparticle Research
Issue Date:
17-May-2013
DOI:
10.1007/s11051-013-1711-5
Type:
Article
ISSN:
13880764
Sponsors:
This study was partially supported by grants from the Ministry of University and Research (MiUR) of Italy (PRIN 2008 grant "miRNA in diagnosis and experimental therapy via nano-vectors of pleural malignant mesothelioma"). The authors thank Dr. M. Vittori Antisari and Dr. R. Marazzi for providing CNT, Dr. Silvia Nuccitelli for help with blood cell characterization, and Prof. L. Campagnolo for invaluable scientific support.
Appears in Collections:
Articles; Physical Sciences and Engineering (PSE) Division; Materials Science and Engineering Program; Solar and Photovoltaic Engineering Research Center (SPERC)

Full metadata record

DC FieldValue Language
dc.contributor.authorDe Nicola, Milena D.en
dc.contributor.authorMirabile Gattia, Danieleen
dc.contributor.authorTraversa, Enricoen
dc.contributor.authorGhibelli, Linaen
dc.date.accessioned2015-08-03T11:04:57Zen
dc.date.available2015-08-03T11:04:57Zen
dc.date.issued2013-05-17en
dc.identifier.issn13880764en
dc.identifier.doi10.1007/s11051-013-1711-5en
dc.identifier.urihttp://hdl.handle.net/10754/562766en
dc.description.abstractThe possibility of exploiting carbon nanotubes (CNT) in biomedical practices requires thorough analysis of the chemical or bulk effects they may exert on the immune system, the complex network that recognizes and eliminates foreign particles. In particular, the phagocytosing ability of cells belonging to the monocyte/macrophage lineage may render these immune cells an ideal toxicological target of pristine CNT, which may form aggregates of size exceeding monocyte/macrophage phagocytosing plasticity. To shed light on this issue, we analyzed the effects that pristine multi-walled CNT (MWCNT) without metal or biological impurities exert on survival and activation of freshly explanted human peripheral blood monocytes, analyzing in parallel the non-phagocytosing lymphocytes, and using graphite as control carbon material. MWCNT (diameter 10-50 nm, length up to 10 μm) exert two different toxic effects on mononuclear leukocytes: a minor apoptogenic effect (on lymphocytes > monocytes), and a major, apoptosis-independent effect that exclusively and deeply affect monocyte homeostasis. Analysis of monocyte number, adhesion, redox equilibrium, and the differentiation markers CD14 and CD11b reveals that MWCNT cause the selective disappearance of phagocytosis-competent monocytes by mechanisms related to the presence of large nanoparticle aggregates, suggesting phenomena of bulk toxicity possibly consisting of frustrated phagocytosis. At the same time, MWCNT stimulate adhesion of the phagocytosis-incompetent monocytes, and their differentiation toward a peculiar maturation asset. These observations point out novel mechanisms of CNT toxicity, renewing concerns that they may impair the innate immune system deranging the inflammatory responses. © 2013 Springer Science+Business Media Dordrecht.en
dc.description.sponsorshipThis study was partially supported by grants from the Ministry of University and Research (MiUR) of Italy (PRIN 2008 grant "miRNA in diagnosis and experimental therapy via nano-vectors of pleural malignant mesothelioma"). The authors thank Dr. M. Vittori Antisari and Dr. R. Marazzi for providing CNT, Dr. Silvia Nuccitelli for help with blood cell characterization, and Prof. L. Campagnolo for invaluable scientific support.en
dc.publisherSpringer Verlagen
dc.subjectCarbon nanotubesen
dc.subjectHomeostasisen
dc.subjectInflammatory responseen
dc.subjectLymphocytesen
dc.subjectMonocytesen
dc.subjectNanotoxicologyen
dc.titleMaturation and demise of human primary monocytes by carbon nanotubesen
dc.typeArticleen
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.contributor.departmentMaterials Science and Engineering Programen
dc.contributor.departmentSolar and Photovoltaic Engineering Research Center (SPERC)en
dc.contributor.departmentMaterials for Energy Conversion and Storage (MECS) Laben
dc.identifier.journalJournal of Nanoparticle Researchen
dc.contributor.institutionDepartment of Biology, University of Rome Tor Vergata, Via della Ricerca Scientifica 1, 00133 Rome, Italyen
dc.contributor.institutionUTTMAT, ENEA-C.R. Casaccia, Rome, Italyen
dc.contributor.institutionDepartment of Chemical Science and Technology, University of Rome Tor Vergata, Rome, Italyen
kaust.authorTraversa, Enricoen
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