Microbial community evolution during simulated managed aquifer recharge in response to different biodegradable dissolved organic carbon (BDOC) concentrations

Handle URI:
http://hdl.handle.net/10754/562739
Title:
Microbial community evolution during simulated managed aquifer recharge in response to different biodegradable dissolved organic carbon (BDOC) concentrations
Authors:
Li, Dong; Alidina, Mazahirali; Ouf, Mohamed; Sharp, Jonathan O.; Saikaly, Pascal ( 0000-0001-7678-3986 ) ; Drewes, Jorg
Abstract:
This study investigates the evolution of the microbial community in laboratory-scale soil columns simulating the infiltration zone of managed aquifer recharge (MAR) systems and analogous natural aquifer sediment ecosystems. Parallel systems were supplemented with either moderate (1.1 mg/L) or low (0.5 mg/L) biodegradable dissolved organic carbon (BDOC) for a period of six months during which time, spatial (1 cm, 30 cm, 60 cm, 90 cm, and 120 cm) and temporal (monthly) analyses of sediment-associated microbial community structure were analyzed. Total microbial biomass associated with sediments was positively correlated with BDOC concentration where a significant decline in BDOC was observed along the column length. Analysis of 16S rRNA genes indicated dominance by Bacteria with Archaea comprising less than 1 percent of the total community. Proteobacteria was found to be the major phylum in samples from all column depths with contributions from Betaproteobacteria, Alphaproteobacteria and Gammaproteobacteria. Microbial community structure at all the phylum, class and genus levels differed significantly at 1 cm between columns receiving moderate and low BDOC concentrations; in contrast strong similarities were observed both between parallel column systems and across samples from 30 to 120 cm depths. Samples from 1 cm depth of the low BDOC columns exhibited higher microbial diversity (expressed as Shannon Index) than those at 1 cm of moderate BDOC columns, and both increased from 5.4 to 5.9 at 1 cm depth to 6.7-8.3 at 30-120 cm depths. The microbial community structure reached steady state after 3-4 months since the initiation of the experiment, which also resulted in an improved DOC removal during the same time period. This study suggested that BDOC could significantly influence microbial community structure regarding both composition and diversity of artificial MAR systems and analogous natural aquifer sediment ecosystems. © 2013 Elsevier Ltd.
KAUST Department:
Water Desalination and Reuse Research Center (WDRC); Biological and Environmental Sciences and Engineering (BESE) Division; Environmental Science and Engineering Program; Water Desalination & Reuse Research Cntr; Environmental Biotechnology Research Group
Publisher:
Elsevier
Journal:
Water Research
Issue Date:
May-2013
DOI:
10.1016/j.watres.2013.02.012
PubMed ID:
23490107
Type:
Article
ISSN:
00431354
Sponsors:
This research was supported by discretionary investigator funds (J.D., P.S.) at King Abdullah University of Science and Technology (KAUST). The material presented is also based in part upon work supported by the National Science Foundation under Grant No. CBET-1055396 (J.S.) and Cooperative Agreement EEC-1028968 (J.D. and J.S.). The authors are thankful for technical assistance provided by Shahjahan Ali and Mohammed S Alarawi at the Biosciences Core Laboratory, King Abdullah University of Science and Technology.
Appears in Collections:
Articles; Environmental Science and Engineering Program; Water Desalination and Reuse Research Center (WDRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorLi, Dongen
dc.contributor.authorAlidina, Mazahiralien
dc.contributor.authorOuf, Mohameden
dc.contributor.authorSharp, Jonathan O.en
dc.contributor.authorSaikaly, Pascalen
dc.contributor.authorDrewes, Jorgen
dc.date.accessioned2015-08-03T11:03:54Zen
dc.date.available2015-08-03T11:03:54Zen
dc.date.issued2013-05en
dc.identifier.issn00431354en
dc.identifier.pmid23490107en
dc.identifier.doi10.1016/j.watres.2013.02.012en
dc.identifier.urihttp://hdl.handle.net/10754/562739en
dc.description.abstractThis study investigates the evolution of the microbial community in laboratory-scale soil columns simulating the infiltration zone of managed aquifer recharge (MAR) systems and analogous natural aquifer sediment ecosystems. Parallel systems were supplemented with either moderate (1.1 mg/L) or low (0.5 mg/L) biodegradable dissolved organic carbon (BDOC) for a period of six months during which time, spatial (1 cm, 30 cm, 60 cm, 90 cm, and 120 cm) and temporal (monthly) analyses of sediment-associated microbial community structure were analyzed. Total microbial biomass associated with sediments was positively correlated with BDOC concentration where a significant decline in BDOC was observed along the column length. Analysis of 16S rRNA genes indicated dominance by Bacteria with Archaea comprising less than 1 percent of the total community. Proteobacteria was found to be the major phylum in samples from all column depths with contributions from Betaproteobacteria, Alphaproteobacteria and Gammaproteobacteria. Microbial community structure at all the phylum, class and genus levels differed significantly at 1 cm between columns receiving moderate and low BDOC concentrations; in contrast strong similarities were observed both between parallel column systems and across samples from 30 to 120 cm depths. Samples from 1 cm depth of the low BDOC columns exhibited higher microbial diversity (expressed as Shannon Index) than those at 1 cm of moderate BDOC columns, and both increased from 5.4 to 5.9 at 1 cm depth to 6.7-8.3 at 30-120 cm depths. The microbial community structure reached steady state after 3-4 months since the initiation of the experiment, which also resulted in an improved DOC removal during the same time period. This study suggested that BDOC could significantly influence microbial community structure regarding both composition and diversity of artificial MAR systems and analogous natural aquifer sediment ecosystems. © 2013 Elsevier Ltd.en
dc.description.sponsorshipThis research was supported by discretionary investigator funds (J.D., P.S.) at King Abdullah University of Science and Technology (KAUST). The material presented is also based in part upon work supported by the National Science Foundation under Grant No. CBET-1055396 (J.S.) and Cooperative Agreement EEC-1028968 (J.D. and J.S.). The authors are thankful for technical assistance provided by Shahjahan Ali and Mohammed S Alarawi at the Biosciences Core Laboratory, King Abdullah University of Science and Technology.en
dc.publisherElsevieren
dc.subjectDNA extractionen
dc.subjectManaged aquifer rechargeen
dc.subjectMicrobial adaptationen
dc.subjectMicrobial community diversityen
dc.subjectPyrosequencingen
dc.subjectWater reuseen
dc.titleMicrobial community evolution during simulated managed aquifer recharge in response to different biodegradable dissolved organic carbon (BDOC) concentrationsen
dc.typeArticleen
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)en
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentEnvironmental Science and Engineering Programen
dc.contributor.departmentWater Desalination & Reuse Research Cntren
dc.contributor.departmentEnvironmental Biotechnology Research Groupen
dc.identifier.journalWater Researchen
dc.contributor.institutionNSF Engineering Research Center ReNUWIt, Department of Civil and Environmental Engineering, Colorado School of Mines, Golden, CO, United Statesen
kaust.authorLi, Dongen
kaust.authorAlidina, Mazahiralien
kaust.authorOuf, Mohameden
kaust.authorSaikaly, Pascalen
kaust.authorDrewes, Jorgen
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