General anaesthetics do not impair developmental expression of the KCC2 potassium-chloride cotransporter in neonatal rats during the brain growth spurt

Handle URI:
http://hdl.handle.net/10754/562689
Title:
General anaesthetics do not impair developmental expression of the KCC2 potassium-chloride cotransporter in neonatal rats during the brain growth spurt
Authors:
Lacoh, Claudia Marvine; Bodogan, T.; Kaila, Kai K.; Fiumelli, Hubert ( 0000-0001-5136-3045 ) ; Vutskits, László
Abstract:
BackgroundThe developmental transition from depolarizing to hyperpolarizing γ-aminobutyric acid-mediated neurotransmission is primarily mediated by an increase in the amount of the potassium-chloride cotransporter KCC2 during early postnatal life. However, it is not known whether early neuronal activity plays a modulatory role in the expression of total KCC2 mRNA and protein in the immature brain. As general anaesthetics are powerful modulators of neuronal activity, the purpose of this study was to explore how these drugs affect KCC2 expression during the brain growth spurt.MethodsWistar rat pups were exposed to either a single dose or 6 h of midazolam, propofol, or ketamine anaesthesia at postnatal days 0, 5, 10, or 15. KCC2 expression was assessed using immunoblotting, immunohistochemistry, or quantitative polymerase chain reaction analysis up to 3 days post-exposure in the medial prefrontal cortex.ResultsThere was a progressive and steep increase in the expression of KCC2 between birth and 2 weeks of age. Exposure to midazolam, propofol, or ketamine up to 6 h at any investigated stages of the brain growth spurt did not influence the expression of this cotransporter protein.ConclusionI.V. general anaesthetics do not seem to influence developmental expression of KCC2 during the brain growth spurt. © 2013 © The Author [2013].
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Publisher:
Oxford University Press (OUP)
Journal:
British Journal of Anaesthesia
Issue Date:
26-Mar-2013
DOI:
10.1093/bja/aet063
PubMed ID:
23533251
Type:
Article
ISSN:
00070912
Sponsors:
This work was founded by the Swiss National Science Foundation (grant 31003A-130625 to L. V.) and by the Academy of Finland and the Letten Foundation (to K.K.).
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorLacoh, Claudia Marvineen
dc.contributor.authorBodogan, T.en
dc.contributor.authorKaila, Kai K.en
dc.contributor.authorFiumelli, Huberten
dc.contributor.authorVutskits, Lászlóen
dc.date.accessioned2015-08-03T11:01:35Zen
dc.date.available2015-08-03T11:01:35Zen
dc.date.issued2013-03-26en
dc.identifier.issn00070912en
dc.identifier.pmid23533251en
dc.identifier.doi10.1093/bja/aet063en
dc.identifier.urihttp://hdl.handle.net/10754/562689en
dc.description.abstractBackgroundThe developmental transition from depolarizing to hyperpolarizing γ-aminobutyric acid-mediated neurotransmission is primarily mediated by an increase in the amount of the potassium-chloride cotransporter KCC2 during early postnatal life. However, it is not known whether early neuronal activity plays a modulatory role in the expression of total KCC2 mRNA and protein in the immature brain. As general anaesthetics are powerful modulators of neuronal activity, the purpose of this study was to explore how these drugs affect KCC2 expression during the brain growth spurt.MethodsWistar rat pups were exposed to either a single dose or 6 h of midazolam, propofol, or ketamine anaesthesia at postnatal days 0, 5, 10, or 15. KCC2 expression was assessed using immunoblotting, immunohistochemistry, or quantitative polymerase chain reaction analysis up to 3 days post-exposure in the medial prefrontal cortex.ResultsThere was a progressive and steep increase in the expression of KCC2 between birth and 2 weeks of age. Exposure to midazolam, propofol, or ketamine up to 6 h at any investigated stages of the brain growth spurt did not influence the expression of this cotransporter protein.ConclusionI.V. general anaesthetics do not seem to influence developmental expression of KCC2 during the brain growth spurt. © 2013 © The Author [2013].en
dc.description.sponsorshipThis work was founded by the Swiss National Science Foundation (grant 31003A-130625 to L. V.) and by the Academy of Finland and the Letten Foundation (to K.K.).en
dc.publisherOxford University Press (OUP)en
dc.subjectanaesthesia, paediatricen
dc.subjectbrain, GABAen
dc.subjectneonatesen
dc.subjecttoxicity, neurotoxicityen
dc.titleGeneral anaesthetics do not impair developmental expression of the KCC2 potassium-chloride cotransporter in neonatal rats during the brain growth spurten
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalBritish Journal of Anaesthesiaen
dc.contributor.institutionDepartment of Anesthesiology, Pharmacology and Intensive Care, University Hospital of Geneva, 1211 Geneva, Switzerlanden
dc.contributor.institutionDepartment of Fundamental Neuroscience, University of Geneva Medical School, 1204 Geneva, Switzerlanden
dc.contributor.institutionDepartment of Biosciences, Finlanden
dc.contributor.institutionNeuroscience Center, University of Helsinki, 00014 Helsinki, Finlanden
kaust.authorFiumelli, Huberten
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