High throughput generation and trapping of individual agarose microgel using microfluidic approach

Handle URI:
http://hdl.handle.net/10754/562664
Title:
High throughput generation and trapping of individual agarose microgel using microfluidic approach
Authors:
Shi, Yang; Gao, Xinghua; Chen, Longqing; Zhang, Min; Ma, Jingyun; Zhang, Xixiang ( 0000-0002-3478-6414 ) ; Qin, Jianhua
Abstract:
Microgel is a kind of biocompatible polymeric material, which has been widely used as micro-carriers in materials synthesis, drug delivery and cell biology applications. However, high-throughput generation of individual microgel for on-site analysis in a microdevice still remains a challenge. Here, we presented a simple and stable droplet microfluidic system to realize high-throughput generation and trapping of individual agarose microgels based on the synergetic effect of surface tension and hydrodynamic forces in microchannels and used it for 3-D cell culture in real-time. The established system was mainly composed of droplet generators with flow focusing T-junction and a series of array individual trap structures. The whole process including the independent agarose microgel formation, immobilization in trapping array and gelation in situ via temperature cooling could be realized on the integrated microdevice completely. The performance of this system was demonstrated by successfully encapsulating and culturing adenoid cystic carcinoma (ACCM) cells in the gelated agarose microgels. This established approach is simple, easy to operate, which can not only generate the micro-carriers with different components in parallel, but also monitor the cell behavior in 3D matrix in real-time. It can also be extended for applications in the area of material synthesis and tissue engineering. © 2013 Springer-Verlag Berlin Heidelberg.
KAUST Department:
Advanced Nanofabrication, Imaging and Characterization Core Lab; Physical Sciences and Engineering (PSE) Division; Materials Science and Engineering Program; Core Labs
Publisher:
Springer Verlag
Journal:
Microfluidics and Nanofluidics
Issue Date:
28-Feb-2013
DOI:
10.1007/s10404-013-1160-6
Type:
Article
ISSN:
16134982
Sponsors:
This research was supported by the Joint Research Fund of NSFC-RGC (11161160522, N_HKUST601/11), Knowledge Innovation Program of the Chinese Academy of Sciences (KJCX2-YW-H18), and Instrument Research and Development Program of the Chinese Academy of Sciences (YZ200908).
Appears in Collections:
Articles; Advanced Nanofabrication, Imaging and Characterization Core Lab; Physical Sciences and Engineering (PSE) Division; Materials Science and Engineering Program

Full metadata record

DC FieldValue Language
dc.contributor.authorShi, Yangen
dc.contributor.authorGao, Xinghuaen
dc.contributor.authorChen, Longqingen
dc.contributor.authorZhang, Minen
dc.contributor.authorMa, Jingyunen
dc.contributor.authorZhang, Xixiangen
dc.contributor.authorQin, Jianhuaen
dc.date.accessioned2015-08-03T11:00:27Zen
dc.date.available2015-08-03T11:00:27Zen
dc.date.issued2013-02-28en
dc.identifier.issn16134982en
dc.identifier.doi10.1007/s10404-013-1160-6en
dc.identifier.urihttp://hdl.handle.net/10754/562664en
dc.description.abstractMicrogel is a kind of biocompatible polymeric material, which has been widely used as micro-carriers in materials synthesis, drug delivery and cell biology applications. However, high-throughput generation of individual microgel for on-site analysis in a microdevice still remains a challenge. Here, we presented a simple and stable droplet microfluidic system to realize high-throughput generation and trapping of individual agarose microgels based on the synergetic effect of surface tension and hydrodynamic forces in microchannels and used it for 3-D cell culture in real-time. The established system was mainly composed of droplet generators with flow focusing T-junction and a series of array individual trap structures. The whole process including the independent agarose microgel formation, immobilization in trapping array and gelation in situ via temperature cooling could be realized on the integrated microdevice completely. The performance of this system was demonstrated by successfully encapsulating and culturing adenoid cystic carcinoma (ACCM) cells in the gelated agarose microgels. This established approach is simple, easy to operate, which can not only generate the micro-carriers with different components in parallel, but also monitor the cell behavior in 3D matrix in real-time. It can also be extended for applications in the area of material synthesis and tissue engineering. © 2013 Springer-Verlag Berlin Heidelberg.en
dc.description.sponsorshipThis research was supported by the Joint Research Fund of NSFC-RGC (11161160522, N_HKUST601/11), Knowledge Innovation Program of the Chinese Academy of Sciences (KJCX2-YW-H18), and Instrument Research and Development Program of the Chinese Academy of Sciences (YZ200908).en
dc.publisherSpringer Verlagen
dc.subject3D cell cultureen
dc.subjectAgaroseen
dc.subjectDropleten
dc.subjectMicrofluidicen
dc.subjectMicrogelen
dc.titleHigh throughput generation and trapping of individual agarose microgel using microfluidic approachen
dc.typeArticleen
dc.contributor.departmentAdvanced Nanofabrication, Imaging and Characterization Core Laben
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.contributor.departmentMaterials Science and Engineering Programen
dc.contributor.departmentCore Labsen
dc.identifier.journalMicrofluidics and Nanofluidicsen
dc.contributor.institutionDalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, Chinaen
kaust.authorChen, Longqingen
kaust.authorZhang, Xixiangen
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