Protein-carbohydrate complex reveals circulating metastatic cells in a microfluidic assay

Handle URI:
http://hdl.handle.net/10754/562651
Title:
Protein-carbohydrate complex reveals circulating metastatic cells in a microfluidic assay
Authors:
Simone, Giuseppina; Malara, Natalia Maria; Trunzo, Valentina; Perozziello, Gerardo; Neužil, Pavel; Francardi, Marco; Roveda, Laura; Renne, Maria; Prati, Ubaldo; Mollace, Vincenzo; Manz, Andreas; Di Fabrizio, Enzo M. ( 0000-0001-5886-4678 )
Abstract:
Advances in carbohydrate sequencing technologies reveal the tremendous complexity of the glycome and the role that glycomics might have to bring insight into the biological functions. Carbohydrate-protein interactions, in particular, are known to be crucial to most mammalian physiological processes as mediators of cell adhesion and metastasis, signal transducers, and organizers of protein interactions. An assay is developed here to mimic the multivalency of biological complexes that selectively and sensitively detect carbohydrate-protein interactions. The binding of β-galactosides and galectin-3 - a protein that is correlated to the progress of tumor and metastasis - is examined. The efficiency of the assay is related to the expression of the receptor while anchoring to the interaction's strength. Comparative binding experiments reveal molecular binding preferences. This study establishes that the assay is robust to isolate metastatic cells from colon affected patients and paves the way to personalized medicine. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Physical Sciences and Engineering (PSE) Division; Materials Science and Engineering Program
Publisher:
Wiley-VCH Verlag
Journal:
Small
Issue Date:
11-Feb-2013
DOI:
10.1002/smll.201202867
PubMed ID:
23401360
Type:
Article
ISSN:
16136810
Sponsors:
This work was partially supported by the project PON "Nuove strategie nanotecnologiche per la messa a punto di farmaci e presidi diagnostici diretti verso cellule cancerose circolanti." (cod. PON01_02782, CUP B71H11000870005), and the project FIRB "Rete Nazionale di Ricerca sulle Nanoscienze ItalNanoNet" (cod. RBPR05JH2P_010, CUP B41J09000110005) granted to the nanotechnology laboratory of the Department of Experimental Medicine of the University of Magna Graecia of Catanzaro, the foundation "T. Campanella" and RSA (ANASTE Calabria).
Appears in Collections:
Articles; Physical Sciences and Engineering (PSE) Division; Materials Science and Engineering Program; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorSimone, Giuseppinaen
dc.contributor.authorMalara, Natalia Mariaen
dc.contributor.authorTrunzo, Valentinaen
dc.contributor.authorPerozziello, Gerardoen
dc.contributor.authorNeužil, Pavelen
dc.contributor.authorFrancardi, Marcoen
dc.contributor.authorRoveda, Lauraen
dc.contributor.authorRenne, Mariaen
dc.contributor.authorPrati, Ubaldoen
dc.contributor.authorMollace, Vincenzoen
dc.contributor.authorManz, Andreasen
dc.contributor.authorDi Fabrizio, Enzo M.en
dc.date.accessioned2015-08-03T10:59:49Zen
dc.date.available2015-08-03T10:59:49Zen
dc.date.issued2013-02-11en
dc.identifier.issn16136810en
dc.identifier.pmid23401360en
dc.identifier.doi10.1002/smll.201202867en
dc.identifier.urihttp://hdl.handle.net/10754/562651en
dc.description.abstractAdvances in carbohydrate sequencing technologies reveal the tremendous complexity of the glycome and the role that glycomics might have to bring insight into the biological functions. Carbohydrate-protein interactions, in particular, are known to be crucial to most mammalian physiological processes as mediators of cell adhesion and metastasis, signal transducers, and organizers of protein interactions. An assay is developed here to mimic the multivalency of biological complexes that selectively and sensitively detect carbohydrate-protein interactions. The binding of β-galactosides and galectin-3 - a protein that is correlated to the progress of tumor and metastasis - is examined. The efficiency of the assay is related to the expression of the receptor while anchoring to the interaction's strength. Comparative binding experiments reveal molecular binding preferences. This study establishes that the assay is robust to isolate metastatic cells from colon affected patients and paves the way to personalized medicine. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.en
dc.description.sponsorshipThis work was partially supported by the project PON "Nuove strategie nanotecnologiche per la messa a punto di farmaci e presidi diagnostici diretti verso cellule cancerose circolanti." (cod. PON01_02782, CUP B71H11000870005), and the project FIRB "Rete Nazionale di Ricerca sulle Nanoscienze ItalNanoNet" (cod. RBPR05JH2P_010, CUP B41J09000110005) granted to the nanotechnology laboratory of the Department of Experimental Medicine of the University of Magna Graecia of Catanzaro, the foundation "T. Campanella" and RSA (ANASTE Calabria).en
dc.publisherWiley-VCH Verlagen
dc.subjectcarbohydratesen
dc.subjectglycomicsen
dc.subjectmetastatic cellsen
dc.subjectmicrofluidic assaysen
dc.subjectproteinsen
dc.titleProtein-carbohydrate complex reveals circulating metastatic cells in a microfluidic assayen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.contributor.departmentMaterials Science and Engineering Programen
dc.identifier.journalSmallen
dc.contributor.institutionKIST Europe, Campus E71, 66123 Saarbrucken, Germanyen
dc.contributor.institutionDepartment of Experimental and Clinical Medicine, Campus S. Venuta, University of Magna Graecia, Germaneto 88100 Catanzaro, Italyen
dc.contributor.institutionOncologic Surgery, Cancer Centre of Excellence T. Campanella Fondation, Germaneto 88100 Catanzaro, Italyen
dc.contributor.institutionFaculty of Pharmacy, Roccelletta di Borgia, University of Magna Graecia, Germaneto 88100 Catanzaro, Italyen
dc.contributor.institutionKIST, Korea Institute for Science and Technology, Hwangno 14 gil 5, Seongbuk-gu, Seoul 136-791, South Koreaen
dc.contributor.institutionIIT (Istituto Italiano di Tecnologia), Via Morego 30, 16163 Genova, Italyen
dc.contributor.institutionCenter for Advanced Biomaterials for Health Care, University of Napoli Federico II, Piazzale Tecchio 80, 80125 Napoli, Italyen
kaust.authorDi Fabrizio, Enzo M.en

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