Detection of bacterial endotoxin in food: New planar interdigital sensors based approach

Handle URI:
http://hdl.handle.net/10754/562645
Title:
Detection of bacterial endotoxin in food: New planar interdigital sensors based approach
Authors:
Abdul Rahman, Mohd Syaifudin; Mukhopadhyay, Subhas Chandra; Yu, Paklam; Goicoechea, J.; Matias, Ignacio R.; Gooneratne, Chinthaka Pasan; Kosel, Jürgen ( 0000-0002-8998-8275 )
Abstract:
Food poisoning caused by endotoxins or Lipopolysaccharide (LPS) are associated with Gram-negative bacteria. Two major food-borne pathogens, Escherichia coli and Salmonella are examples of Gram-negative bacteria which cause a large number of outbreaks of food poisoning. New types of planar interdigital sensors have been fabricated with different coating materials to assess their response to endotoxins. A carboxyl-functional polymer, APTES (3-Aminopropyltriethoxysilane) and Thionine were chosen to be coated onto FR4 interdigital sensors. The chosen coating materials have carboxylic or amine functional groups, which were optimized to be stable in water. All coated sensors were immobilized with PmB (Polymyxin B) which has specific binding properties to LPS. The sensors were tested with different concentrations of LPS O111:B4, ranging from 0.1 to 1000 μg/ml. Analyses of sensors' performance were based on the impedance spectroscopy method. The impedance spectra were modeled using a constant phase-element (CPE) equivalent circuit, and a principal component analysis (PCA) was used for data classification. Sensor coated with APTES has shown better selectivity for LPS detection. The experiments were repeated by coating APTES and immobilizing PmB to a new improve designed of novel interdigital sensors (thin film silicon based sensors). These sensors were observed to have better sensitivity and selectivity to the target biomolecules of LPS. Further experiments were conducted to study the effect of different coating thickness on sensor sensitivity, selectivity and stability. Different food samples contaminated with endotoxin were also tested to verify that the interdigital sensing approach is able to be used for endotoxin detection. © 2012 Elsevier Ltd. All rights reserved.
KAUST Department:
Sensing, Magnetism and Microsystems Lab; Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division; Electrical Engineering Program
Publisher:
Elsevier
Journal:
Journal of Food Engineering
Issue Date:
Feb-2013
DOI:
10.1016/j.jfoodeng.2012.08.026
Type:
Article
ISSN:
02608774
Appears in Collections:
Articles; Electrical Engineering Program; Sensing, Magnetism and Microsystems Lab; Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorAbdul Rahman, Mohd Syaifudinen
dc.contributor.authorMukhopadhyay, Subhas Chandraen
dc.contributor.authorYu, Paklamen
dc.contributor.authorGoicoechea, J.en
dc.contributor.authorMatias, Ignacio R.en
dc.contributor.authorGooneratne, Chinthaka Pasanen
dc.contributor.authorKosel, Jürgenen
dc.date.accessioned2015-08-03T10:59:35Zen
dc.date.available2015-08-03T10:59:35Zen
dc.date.issued2013-02en
dc.identifier.issn02608774en
dc.identifier.doi10.1016/j.jfoodeng.2012.08.026en
dc.identifier.urihttp://hdl.handle.net/10754/562645en
dc.description.abstractFood poisoning caused by endotoxins or Lipopolysaccharide (LPS) are associated with Gram-negative bacteria. Two major food-borne pathogens, Escherichia coli and Salmonella are examples of Gram-negative bacteria which cause a large number of outbreaks of food poisoning. New types of planar interdigital sensors have been fabricated with different coating materials to assess their response to endotoxins. A carboxyl-functional polymer, APTES (3-Aminopropyltriethoxysilane) and Thionine were chosen to be coated onto FR4 interdigital sensors. The chosen coating materials have carboxylic or amine functional groups, which were optimized to be stable in water. All coated sensors were immobilized with PmB (Polymyxin B) which has specific binding properties to LPS. The sensors were tested with different concentrations of LPS O111:B4, ranging from 0.1 to 1000 μg/ml. Analyses of sensors' performance were based on the impedance spectroscopy method. The impedance spectra were modeled using a constant phase-element (CPE) equivalent circuit, and a principal component analysis (PCA) was used for data classification. Sensor coated with APTES has shown better selectivity for LPS detection. The experiments were repeated by coating APTES and immobilizing PmB to a new improve designed of novel interdigital sensors (thin film silicon based sensors). These sensors were observed to have better sensitivity and selectivity to the target biomolecules of LPS. Further experiments were conducted to study the effect of different coating thickness on sensor sensitivity, selectivity and stability. Different food samples contaminated with endotoxin were also tested to verify that the interdigital sensing approach is able to be used for endotoxin detection. © 2012 Elsevier Ltd. All rights reserved.en
dc.publisherElsevieren
dc.subjectBacterial endotoxinen
dc.subjectFood poisoningen
dc.subjectImpedance spectroscopyen
dc.subjectInterdigital sensorsen
dc.subjectPrincipal component analysisen
dc.titleDetection of bacterial endotoxin in food: New planar interdigital sensors based approachen
dc.typeArticleen
dc.contributor.departmentSensing, Magnetism and Microsystems Laben
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Divisionen
dc.contributor.departmentElectrical Engineering Programen
dc.identifier.journalJournal of Food Engineeringen
dc.contributor.institutionSchool of Engineering and Advanced Technology, Massey University, Palmerston North, New Zealanden
dc.contributor.institutionDepartment of Electrical and Electronic Engineering, Public University of Navarra, Pamplona, Spainen
dc.contributor.institutionMalaysian Agricultural Research and Development Institute, Serdang 43300, Selangor, Malaysiaen
kaust.authorGooneratne, Chinthaka Pasanen
kaust.authorKosel, Jürgenen
All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.