Reactor performance in terms of COD and nitrogen removal and bacterial community structure of a three-stage rotating bioelectrochemical contactor

Handle URI:
http://hdl.handle.net/10754/562624
Title:
Reactor performance in terms of COD and nitrogen removal and bacterial community structure of a three-stage rotating bioelectrochemical contactor
Authors:
Sayess, Rassil R.; Saikaly, Pascal ( 0000-0001-7678-3986 ) ; El-Fadel, Mutasem E.; Li, Dong; Semerjian, Lucy A.
Abstract:
Integrating microbial fuel cell (MFC) into rotating biological contactor (RBC) creates an opportunity for enhanced removal of COD and nitrogen coupled with energy generation from wastewater. In this study, a three-stage rotating bioelectrochemical contactor (referred to as RBC-MFC unit) integrating MFC with RBC technology was constructed for simultaneous removal of carbonaceous and nitrogenous compounds and electricity generation from a synthetic medium containing acetate and ammonium. The performance of the RBC-MFC unit was compared to a control reactor (referred to as RBC unit) that was operated under the same conditions but without current generation (i.e. open-circuit mode). The effect of hydraulic loading rate (HLR) and COD/N ratio on the performance of the two units was investigated. At low (3.05 gCOD g-1N) and high COD/N ratio (6.64 gCOD g-1N), both units achieved almost similar COD and ammonia-nitrogen removal. However, the RBC-MFC unit achieved significantly higher denitrification and nitrogen removal compared to the RBC unit indicating improved denitrification at the cathode due to current flow. The average voltage under 1000 Ω external resistance ranged between 0.03 and 0.30 V and between 0.02 and 0.21 V for stages 1 and 2 of the RBC-MFC unit. Pyrosequencing analysis of bacterial 16S rRNA gene revealed high bacterial diversity at the anode and cathode of both units. Genera that play a role in nitrification (Nitrospira; Nitrosomonas), denitrification (Comamonas; Thauera) and electricity generation (Geobacter) were identified at the electrodes. Geobacter was only detected on the anode of the RBC-MFC unit. Nitrifiers and denitrifiers were more abundant in the RBC-MFC unit compared to the RBC unit and were largely present on the cathode of both units suggesting that most of the nitrogen removal occurred at the cathode. © 2012 Elsevier Ltd.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Water Desalination and Reuse Research Center (WDRC); Environmental Science and Engineering Program; Water Desalination & Reuse Research Cntr; Environmental Biotechnology Research Group
Publisher:
Elsevier
Journal:
Water Research
Issue Date:
Feb-2013
DOI:
10.1016/j.watres.2012.11.023
PubMed ID:
23219389
Type:
Article
ISSN:
00431354
Sponsors:
This research was supported by discretionary investigator funds (P.S.) at King Abdullah University of Science and Technology (KAUST). The material presented is also based in part upon work supported by the University Research Board at the American University of Beirut. Special thanks are extended to Mohammed Alarawi and Shahjahan Ali at the Bioscience Core Laboratory at KAUST.
Appears in Collections:
Articles; Environmental Science and Engineering Program; Water Desalination and Reuse Research Center (WDRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorSayess, Rassil R.en
dc.contributor.authorSaikaly, Pascalen
dc.contributor.authorEl-Fadel, Mutasem E.en
dc.contributor.authorLi, Dongen
dc.contributor.authorSemerjian, Lucy A.en
dc.date.accessioned2015-08-03T10:58:49Zen
dc.date.available2015-08-03T10:58:49Zen
dc.date.issued2013-02en
dc.identifier.issn00431354en
dc.identifier.pmid23219389en
dc.identifier.doi10.1016/j.watres.2012.11.023en
dc.identifier.urihttp://hdl.handle.net/10754/562624en
dc.description.abstractIntegrating microbial fuel cell (MFC) into rotating biological contactor (RBC) creates an opportunity for enhanced removal of COD and nitrogen coupled with energy generation from wastewater. In this study, a three-stage rotating bioelectrochemical contactor (referred to as RBC-MFC unit) integrating MFC with RBC technology was constructed for simultaneous removal of carbonaceous and nitrogenous compounds and electricity generation from a synthetic medium containing acetate and ammonium. The performance of the RBC-MFC unit was compared to a control reactor (referred to as RBC unit) that was operated under the same conditions but without current generation (i.e. open-circuit mode). The effect of hydraulic loading rate (HLR) and COD/N ratio on the performance of the two units was investigated. At low (3.05 gCOD g-1N) and high COD/N ratio (6.64 gCOD g-1N), both units achieved almost similar COD and ammonia-nitrogen removal. However, the RBC-MFC unit achieved significantly higher denitrification and nitrogen removal compared to the RBC unit indicating improved denitrification at the cathode due to current flow. The average voltage under 1000 Ω external resistance ranged between 0.03 and 0.30 V and between 0.02 and 0.21 V for stages 1 and 2 of the RBC-MFC unit. Pyrosequencing analysis of bacterial 16S rRNA gene revealed high bacterial diversity at the anode and cathode of both units. Genera that play a role in nitrification (Nitrospira; Nitrosomonas), denitrification (Comamonas; Thauera) and electricity generation (Geobacter) were identified at the electrodes. Geobacter was only detected on the anode of the RBC-MFC unit. Nitrifiers and denitrifiers were more abundant in the RBC-MFC unit compared to the RBC unit and were largely present on the cathode of both units suggesting that most of the nitrogen removal occurred at the cathode. © 2012 Elsevier Ltd.en
dc.description.sponsorshipThis research was supported by discretionary investigator funds (P.S.) at King Abdullah University of Science and Technology (KAUST). The material presented is also based in part upon work supported by the University Research Board at the American University of Beirut. Special thanks are extended to Mohammed Alarawi and Shahjahan Ali at the Bioscience Core Laboratory at KAUST.en
dc.publisherElsevieren
dc.subject16S rRNA gene pyrosequencingen
dc.subjectDenitrificationen
dc.subjectMicrobial fuel cellen
dc.subjectNitrificationen
dc.subjectRotating bioelectrochemical contactoren
dc.subjectRotating biological contactoren
dc.titleReactor performance in terms of COD and nitrogen removal and bacterial community structure of a three-stage rotating bioelectrochemical contactoren
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)en
dc.contributor.departmentEnvironmental Science and Engineering Programen
dc.contributor.departmentWater Desalination & Reuse Research Cntren
dc.contributor.departmentEnvironmental Biotechnology Research Groupen
dc.identifier.journalWater Researchen
dc.contributor.institutionDepartment of Civil and Environmental Engineering, American University of Beirut, Beirut, Lebanonen
kaust.authorSaikaly, Pascalen
kaust.authorLi, Dongen
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