Raman database of amino acids solutions: A critical study of Extended Multiplicative Signal Correction

Handle URI:
http://hdl.handle.net/10754/562547
Title:
Raman database of amino acids solutions: A critical study of Extended Multiplicative Signal Correction
Authors:
Candeloro, Patrizio; Grande, Elisabetta; Raimondo, Raffaella; Di Mascolo, Daniele; Gentile, Francesco T.; Coluccio, Maria Laura; Perozziello, Gerardo; Malara, Natalia Maria; Francardi, Marco; Di Fabrizio, Enzo M. ( 0000-0001-5886-4678 )
Abstract:
The Raman spectra of biological materials always exhibit complex profiles, constituting several peaks and/or bands which arise due to the large variety of biomolecules. The extraction of quantitative information from these spectra is not a trivial task. While qualitative information can be retrieved from the changes in peaks frequencies or from the appearance/disappearance of some peaks, quantitative analysis requires an examination of peak intensities. Unfortunately in biological samples it is not easy to identify a reference peak for normalizing intensities, and this makes it very difficult to study the peak intensities. In the last decades a more refined mathematical tool, the extended multiplicative signal correction (EMSC), has been proposed for treating infrared spectra, which is also capable of providing quantitative information. From the mathematical and physical point of view, EMSC can also be applied to Raman spectra, as recently proposed. In this work the reliability of the EMSC procedure is tested by application to a well defined biological system: the 20 standard amino acids and their combination in peptides. The first step is the collection of a Raman database of these 20 amino acids, and subsequently EMSC processing is applied to retrieve quantitative information from amino acids mixtures and peptides. A critical review of the results is presented, showing that EMSC has to be carefully handled for complex biological systems. © 2013 The Royal Society of Chemistry.
KAUST Department:
Physical Sciences and Engineering (PSE) Division; Materials Science and Engineering Program
Publisher:
Royal Society of Chemistry
Journal:
Analyst
Issue Date:
2013
DOI:
10.1039/c3an01665j
PubMed ID:
24153318
Type:
Article
ISSN:
00032654
Sponsors:
The authors gratefully acknowledge Dr Camillo Palmieri for providing the peptides IBTK1 and IBTK2. Financial support is acknowledged from Italian-FIRB project "Rete Nazionale di Ricerca sulle Nanoscienze ItalNanoNet" (cod. RBPR05JH2P_010, CUP B41J09000110005) and from Cariplo Foundation under the project "New Frontiers in Plasmonic Nanosensing" (Grant no. 2011-0338).
Appears in Collections:
Articles; Physical Sciences and Engineering (PSE) Division; Materials Science and Engineering Program

Full metadata record

DC FieldValue Language
dc.contributor.authorCandeloro, Patrizioen
dc.contributor.authorGrande, Elisabettaen
dc.contributor.authorRaimondo, Raffaellaen
dc.contributor.authorDi Mascolo, Danieleen
dc.contributor.authorGentile, Francesco T.en
dc.contributor.authorColuccio, Maria Lauraen
dc.contributor.authorPerozziello, Gerardoen
dc.contributor.authorMalara, Natalia Mariaen
dc.contributor.authorFrancardi, Marcoen
dc.contributor.authorDi Fabrizio, Enzo M.en
dc.date.accessioned2015-08-03T10:42:11Zen
dc.date.available2015-08-03T10:42:11Zen
dc.date.issued2013en
dc.identifier.issn00032654en
dc.identifier.pmid24153318en
dc.identifier.doi10.1039/c3an01665jen
dc.identifier.urihttp://hdl.handle.net/10754/562547en
dc.description.abstractThe Raman spectra of biological materials always exhibit complex profiles, constituting several peaks and/or bands which arise due to the large variety of biomolecules. The extraction of quantitative information from these spectra is not a trivial task. While qualitative information can be retrieved from the changes in peaks frequencies or from the appearance/disappearance of some peaks, quantitative analysis requires an examination of peak intensities. Unfortunately in biological samples it is not easy to identify a reference peak for normalizing intensities, and this makes it very difficult to study the peak intensities. In the last decades a more refined mathematical tool, the extended multiplicative signal correction (EMSC), has been proposed for treating infrared spectra, which is also capable of providing quantitative information. From the mathematical and physical point of view, EMSC can also be applied to Raman spectra, as recently proposed. In this work the reliability of the EMSC procedure is tested by application to a well defined biological system: the 20 standard amino acids and their combination in peptides. The first step is the collection of a Raman database of these 20 amino acids, and subsequently EMSC processing is applied to retrieve quantitative information from amino acids mixtures and peptides. A critical review of the results is presented, showing that EMSC has to be carefully handled for complex biological systems. © 2013 The Royal Society of Chemistry.en
dc.description.sponsorshipThe authors gratefully acknowledge Dr Camillo Palmieri for providing the peptides IBTK1 and IBTK2. Financial support is acknowledged from Italian-FIRB project "Rete Nazionale di Ricerca sulle Nanoscienze ItalNanoNet" (cod. RBPR05JH2P_010, CUP B41J09000110005) and from Cariplo Foundation under the project "New Frontiers in Plasmonic Nanosensing" (Grant no. 2011-0338).en
dc.publisherRoyal Society of Chemistryen
dc.titleRaman database of amino acids solutions: A critical study of Extended Multiplicative Signal Correctionen
dc.typeArticleen
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.contributor.departmentMaterials Science and Engineering Programen
dc.identifier.journalAnalysten
dc.contributor.institutionBioNEM Laboratory, Experimental and Clinical Medicine Department, University Magna Graecia of Catanzaro, 88100 Loc. Germaneto, Catanzaro, Italyen
dc.contributor.institutionItalian Institute of Technology (IIT), via Barsanti, 73010 Arnesano, Lecce, Italyen
kaust.authorDi Fabrizio, Enzo M.en

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