Hydrophilic interaction chromatography coupled matrix assisted laser desorption/ionization mass spectrometry for molecular analysis of organic compounds in medicines, tea, and coffee

Handle URI:
http://hdl.handle.net/10754/562537
Title:
Hydrophilic interaction chromatography coupled matrix assisted laser desorption/ionization mass spectrometry for molecular analysis of organic compounds in medicines, tea, and coffee
Authors:
Wang, Renqi; Bao, Kai; Croue, Jean-Philippe; Ng, Siuchoon
Abstract:
Natural occurring organic compounds from food, natural organic matter, as well as metabolic products have received intense attention in current chemical and biological studies. Examination of unknown compounds in complex sample matrices is hampered by the limited choices for data readout and molecular elucidation. Herein, we report a generic method of hydrophilic interaction chromatography (HILIC) coupled with matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS) for the rapid characterization of ingredients in pharmaceutical compounds, tea, and coffee. The analytes were first fractionated using a cationic HILIC column prior to MALDI-MS analyses. It was found that the retention times of a compound arising from different samples were consistent under the same conditions. Accordingly, molecules can be readily characterized by both the mass and chromatographic retention time. The retention behaviors of acidic and basic compounds on the cationic HILIC column were found to be significantly influenced by the pH of mobile phases, whereas neutral compounds depicted a constant retention time at different pH. The general HILIC-MALDI-MS method is feasible for fast screening of naturally occurring organic compounds. A series of homologs can be determined if they have the same retention behavior. Their structural features can be elucidated by considering their mass differences and hydrophilic properties as determined by HILIC chromatogram. © 2013 The Royal Society of Chemistry.
KAUST Department:
Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division; Water Desalination and Reuse Research Center (WDRC); Biological and Environmental Sciences and Engineering (BESE) Division
Publisher:
Royal Society of Chemistry
Journal:
Analyst
Issue Date:
2013
DOI:
10.1039/c3an01425h
PubMed ID:
24045409
Type:
Article
ISSN:
00032654
Sponsors:
The authors are grateful for funding from A*STAR (SERC GRANT no.: 0921010056)-M47120013 and the starting-funding of Lanzhou University.
Appears in Collections:
Articles; Water Desalination and Reuse Research Center (WDRC); Biological and Environmental Sciences and Engineering (BESE) Division; Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorWang, Renqien
dc.contributor.authorBao, Kaien
dc.contributor.authorCroue, Jean-Philippeen
dc.contributor.authorNg, Siuchoonen
dc.date.accessioned2015-08-03T10:41:48Zen
dc.date.available2015-08-03T10:41:48Zen
dc.date.issued2013en
dc.identifier.issn00032654en
dc.identifier.pmid24045409en
dc.identifier.doi10.1039/c3an01425hen
dc.identifier.urihttp://hdl.handle.net/10754/562537en
dc.description.abstractNatural occurring organic compounds from food, natural organic matter, as well as metabolic products have received intense attention in current chemical and biological studies. Examination of unknown compounds in complex sample matrices is hampered by the limited choices for data readout and molecular elucidation. Herein, we report a generic method of hydrophilic interaction chromatography (HILIC) coupled with matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS) for the rapid characterization of ingredients in pharmaceutical compounds, tea, and coffee. The analytes were first fractionated using a cationic HILIC column prior to MALDI-MS analyses. It was found that the retention times of a compound arising from different samples were consistent under the same conditions. Accordingly, molecules can be readily characterized by both the mass and chromatographic retention time. The retention behaviors of acidic and basic compounds on the cationic HILIC column were found to be significantly influenced by the pH of mobile phases, whereas neutral compounds depicted a constant retention time at different pH. The general HILIC-MALDI-MS method is feasible for fast screening of naturally occurring organic compounds. A series of homologs can be determined if they have the same retention behavior. Their structural features can be elucidated by considering their mass differences and hydrophilic properties as determined by HILIC chromatogram. © 2013 The Royal Society of Chemistry.en
dc.description.sponsorshipThe authors are grateful for funding from A*STAR (SERC GRANT no.: 0921010056)-M47120013 and the starting-funding of Lanzhou University.en
dc.publisherRoyal Society of Chemistryen
dc.titleHydrophilic interaction chromatography coupled matrix assisted laser desorption/ionization mass spectrometry for molecular analysis of organic compounds in medicines, tea, and coffeeen
dc.typeArticleen
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Divisionen
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)en
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalAnalysten
dc.contributor.institutionCollege of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, Chinaen
dc.contributor.institutionSchool of Chemical and Biomolecular Engineering, Nanyang Technological University, 62 Nanyang Drive, Singapore 637459, Singaporeen
kaust.authorBao, Kaien
kaust.authorCroue, Jean-Philippeen
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