Racemization of enantiopure secondary alcohols by Thermoanaerobacter ethanolicus secondary alcohol dehydrogenase

Handle URI:
http://hdl.handle.net/10754/562489
Title:
Racemization of enantiopure secondary alcohols by Thermoanaerobacter ethanolicus secondary alcohol dehydrogenase
Authors:
Musa, Musa M.; Phillips, Robert S.; Laivenieks, Maris; Vieille, Claire; Takahashi, Masateru; Hamdan, Samir ( 0000-0001-5192-1852 )
Abstract:
Controlled racemization of enantiopure phenyl-ring-containing secondary alcohols is achieved in this study using W110A secondary alcohol dehydrogenase from Thermoanaerobacter ethanolicus (W110A TeSADH) and in the presence of the reduced and oxidized forms of its cofactor nicotinamide-adenine dinucleotide. Racemization of both enantiomers of alcohols accepted by W110A TeSADH, not only with low, but also with reasonably high, enantiomeric discrimination is achieved by this method. Furthermore, the high tolerance of TeSADH to organic solvents allows TeSADH-catalyzed racemization to be conducted in media containing up to 50% (v/v) of organic solvents. © 2013 The Royal Society of Chemistry.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program
Publisher:
Royal Society of Chemistry (RSC)
Journal:
Organic & Biomolecular Chemistry
Issue Date:
2013
DOI:
10.1039/c3ob27415b
PubMed ID:
23525226
Type:
Article
ISSN:
14770520
Sponsors:
The authors acknowledge the support provided by King Abdulaziz City for Science and Technology (KACST) through the Science and Technology Unit at King Fahd University of Petroleum and Minerals (KFUPM) for funding this work through project no. 11-BIO1666-04 as part of the National Science, Technology and Innovation Plan.
Appears in Collections:
Articles; Bioscience Program; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorMusa, Musa M.en
dc.contributor.authorPhillips, Robert S.en
dc.contributor.authorLaivenieks, Marisen
dc.contributor.authorVieille, Claireen
dc.contributor.authorTakahashi, Masateruen
dc.contributor.authorHamdan, Samiren
dc.date.accessioned2015-08-03T10:39:58Zen
dc.date.available2015-08-03T10:39:58Zen
dc.date.issued2013en
dc.identifier.issn14770520en
dc.identifier.pmid23525226en
dc.identifier.doi10.1039/c3ob27415ben
dc.identifier.urihttp://hdl.handle.net/10754/562489en
dc.description.abstractControlled racemization of enantiopure phenyl-ring-containing secondary alcohols is achieved in this study using W110A secondary alcohol dehydrogenase from Thermoanaerobacter ethanolicus (W110A TeSADH) and in the presence of the reduced and oxidized forms of its cofactor nicotinamide-adenine dinucleotide. Racemization of both enantiomers of alcohols accepted by W110A TeSADH, not only with low, but also with reasonably high, enantiomeric discrimination is achieved by this method. Furthermore, the high tolerance of TeSADH to organic solvents allows TeSADH-catalyzed racemization to be conducted in media containing up to 50% (v/v) of organic solvents. © 2013 The Royal Society of Chemistry.en
dc.description.sponsorshipThe authors acknowledge the support provided by King Abdulaziz City for Science and Technology (KACST) through the Science and Technology Unit at King Fahd University of Petroleum and Minerals (KFUPM) for funding this work through project no. 11-BIO1666-04 as part of the National Science, Technology and Innovation Plan.en
dc.publisherRoyal Society of Chemistry (RSC)en
dc.titleRacemization of enantiopure secondary alcohols by Thermoanaerobacter ethanolicus secondary alcohol dehydrogenaseen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentBioscience Programen
dc.identifier.journalOrganic & Biomolecular Chemistryen
dc.contributor.institutionDepartment of Chemistry, King Fahd University of Petroleum and Minerals, Dhahran 31261, Saudi Arabiaen
dc.contributor.institutionDepartment of Chemistry, University of Georgia, Athens GA 30602-2556, United Statesen
dc.contributor.institutionDepartment of Biochemistry and Molecular Biology, University of Georgia, Athens GA 30602-2556, United Statesen
dc.contributor.institutionDepartment of Microbiology and Molecular Genetics, Michigan State University, East Lansing MI 48824, United Statesen
kaust.authorTakahashi, Masateruen
kaust.authorHamdan, Samiren
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