Characterization of internal structure of hydrated agar and gelatin matrices by cryo-SEM

Handle URI:
http://hdl.handle.net/10754/562467
Title:
Characterization of internal structure of hydrated agar and gelatin matrices by cryo-SEM
Authors:
Rahbani, Janane; Behzad, Ali Reza; Khashab, Niveen M. ( 0000-0003-2728-0666 ) ; Al-Ghoul, Mazen
Abstract:
There has been a considerable interest in recent years in developing polymer gel matrices for many important applications such as 2DE for quantization and separation of a variety of proteins and drug delivery system to control the release of active agents. However, a well-defined knowledge of the ultrastructures of the gels has been elusive. In this study, we report the characterization of two different polymers used in 2DE: Gelatin, a naturally occurring polymer derived from collagen (protein) and agar, a polymer of polysaccharide (sugar) origin. Low-temperature SEM is used to examine the internal structure of these gels in their frozen natural hydrated states. Results of this study show that both polymers have an array of hollow cells that resembles honeycomb structures. While agar pores are almost circular, the corresponding Gaussian curve is very broad exhibiting a range of radii from nearly 370 to 700 nm. Gelatin pores are smaller and more homogeneous reflecting a narrower distribution from nearly 320 to 650 nm. Overall, these ultrastructural findings could be used to correlate with functions of the polymers. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
KAUST Department:
Advanced Nanofabrication, Imaging and Characterization Core Lab; Numerical Porous Media SRI Center (NumPor); Smart Hybrid Materials (SHMs) lab; Physical Sciences and Engineering (PSE) Division; Chemical Science Program; Advanced Membranes and Porous Materials Research Center; Core Labs
Publisher:
Wiley-Blackwell
Journal:
ELECTROPHORESIS
Issue Date:
26-Dec-2012
DOI:
10.1002/elps.201200434
PubMed ID:
23161518
Type:
Article
ISSN:
01730835
Sponsors:
The authors gratefully acknowledge the funding provided by the American University of Beirut Research Board and by the Lebanese National Council for Scientific Research (LCNSR).
Appears in Collections:
Articles; Advanced Membranes and Porous Materials Research Center; Advanced Nanofabrication, Imaging and Characterization Core Lab; Physical Sciences and Engineering (PSE) Division; Controlled Release and Delivery Laboratory; Chemical Science Program

Full metadata record

DC FieldValue Language
dc.contributor.authorRahbani, Jananeen
dc.contributor.authorBehzad, Ali Rezaen
dc.contributor.authorKhashab, Niveen M.en
dc.contributor.authorAl-Ghoul, Mazenen
dc.date.accessioned2015-08-03T10:39:17Zen
dc.date.available2015-08-03T10:39:17Zen
dc.date.issued2012-12-26en
dc.identifier.issn01730835en
dc.identifier.pmid23161518en
dc.identifier.doi10.1002/elps.201200434en
dc.identifier.urihttp://hdl.handle.net/10754/562467en
dc.description.abstractThere has been a considerable interest in recent years in developing polymer gel matrices for many important applications such as 2DE for quantization and separation of a variety of proteins and drug delivery system to control the release of active agents. However, a well-defined knowledge of the ultrastructures of the gels has been elusive. In this study, we report the characterization of two different polymers used in 2DE: Gelatin, a naturally occurring polymer derived from collagen (protein) and agar, a polymer of polysaccharide (sugar) origin. Low-temperature SEM is used to examine the internal structure of these gels in their frozen natural hydrated states. Results of this study show that both polymers have an array of hollow cells that resembles honeycomb structures. While agar pores are almost circular, the corresponding Gaussian curve is very broad exhibiting a range of radii from nearly 370 to 700 nm. Gelatin pores are smaller and more homogeneous reflecting a narrower distribution from nearly 320 to 650 nm. Overall, these ultrastructural findings could be used to correlate with functions of the polymers. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.en
dc.description.sponsorshipThe authors gratefully acknowledge the funding provided by the American University of Beirut Research Board and by the Lebanese National Council for Scientific Research (LCNSR).en
dc.publisherWiley-Blackwellen
dc.subjectAgaren
dc.subjectCryo-SEMen
dc.subjectGelatin-Proteomicsen
dc.titleCharacterization of internal structure of hydrated agar and gelatin matrices by cryo-SEMen
dc.typeArticleen
dc.contributor.departmentAdvanced Nanofabrication, Imaging and Characterization Core Laben
dc.contributor.departmentNumerical Porous Media SRI Center (NumPor)en
dc.contributor.departmentSmart Hybrid Materials (SHMs) laben
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.contributor.departmentChemical Science Programen
dc.contributor.departmentAdvanced Membranes and Porous Materials Research Centeren
dc.contributor.departmentCore Labsen
dc.identifier.journalELECTROPHORESISen
dc.contributor.institutionDepartment of Chemistry, American University of Beirut, Riad El-Solh, Beirut, Lebanonen
kaust.authorBehzad, Ali Rezaen
kaust.authorKhashab, Niveen M.en

Related articles on PubMed

All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.