Adaptation of the genetically tractable malaria pathogen Plasmodium knowlesi to continuous culture in human erythrocytes

Handle URI:
http://hdl.handle.net/10754/562466
Title:
Adaptation of the genetically tractable malaria pathogen Plasmodium knowlesi to continuous culture in human erythrocytes
Authors:
Moon, Robert; Hall, Joanna M.; Rangkuti, Farania Gama Ardhina; Ho, YungShwen; Almond, Neil M.; Mitchell, Graham Howard; Pain, Arnab ( 0000-0002-1755-2819 ) ; Holder, Anthony A.; Blackman, Michael J.
Abstract:
Research into the aetiological agent of the most widespread form of severe malaria, Plasmodium falciparum, has benefitted enormously from the ability to culture and genetically manipulate blood-stage forms of the parasite in vitro. However, most malaria outside Africa is caused by a distinct Plasmodium species, Plasmodium vivax, and it has become increasingly apparent that zoonotic infection by the closely related simian parasite Plasmodium knowlesi is a frequent cause of life-threatening malaria in regions of southeast Asia. Neither of these important malarial species can be cultured in human cells in vitro, requiring access to primates with the associated ethical and practical constraints. We report the successful adaptation of P. knowlesi to continuous culture in human erythrocytes. Human-adapted P. knowlesi clones maintain their capacity to replicate in monkey erythrocytes and can be genetically modified with unprecedented efficiency, providing an important and unique model for studying conserved aspects of malarial biology as well as species-specific features of an emerging pathogen.
KAUST Department:
Computational Bioscience Research Center (CBRC); Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program; Computer Science Program; Pathogen Genomics Laboratory
Publisher:
Proceedings of the National Academy of Sciences
Journal:
Proceedings of the National Academy of Sciences
Issue Date:
24-Dec-2012
DOI:
10.1073/pnas.1216457110
PubMed ID:
23267069
PubMed Central ID:
PMC3545754
Type:
Article
ISSN:
00278424
Sponsors:
This work was supported by the United Kingdom Medical Research Council (U117532063 and U117532067), the European Community's FP7 Programme under Grant Agreement 242095 (EviMalar), and a Medical Research Council Career Development fellowship (to R. W. M.). A. P. was funded by his faculty baseline support and Office for Competitive Research Funds from the King Abdullah University of Science and Technology.
Additional Links:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3545754
Appears in Collections:
Articles; Bioscience Program; Computer Science Program; Computational Bioscience Research Center (CBRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorMoon, Roberten
dc.contributor.authorHall, Joanna M.en
dc.contributor.authorRangkuti, Farania Gama Ardhinaen
dc.contributor.authorHo, YungShwenen
dc.contributor.authorAlmond, Neil M.en
dc.contributor.authorMitchell, Graham Howarden
dc.contributor.authorPain, Arnaben
dc.contributor.authorHolder, Anthony A.en
dc.contributor.authorBlackman, Michael J.en
dc.date.accessioned2015-08-03T10:39:14Zen
dc.date.available2015-08-03T10:39:14Zen
dc.date.issued2012-12-24en
dc.identifier.issn00278424en
dc.identifier.pmid23267069en
dc.identifier.doi10.1073/pnas.1216457110en
dc.identifier.urihttp://hdl.handle.net/10754/562466en
dc.description.abstractResearch into the aetiological agent of the most widespread form of severe malaria, Plasmodium falciparum, has benefitted enormously from the ability to culture and genetically manipulate blood-stage forms of the parasite in vitro. However, most malaria outside Africa is caused by a distinct Plasmodium species, Plasmodium vivax, and it has become increasingly apparent that zoonotic infection by the closely related simian parasite Plasmodium knowlesi is a frequent cause of life-threatening malaria in regions of southeast Asia. Neither of these important malarial species can be cultured in human cells in vitro, requiring access to primates with the associated ethical and practical constraints. We report the successful adaptation of P. knowlesi to continuous culture in human erythrocytes. Human-adapted P. knowlesi clones maintain their capacity to replicate in monkey erythrocytes and can be genetically modified with unprecedented efficiency, providing an important and unique model for studying conserved aspects of malarial biology as well as species-specific features of an emerging pathogen.en
dc.description.sponsorshipThis work was supported by the United Kingdom Medical Research Council (U117532063 and U117532067), the European Community's FP7 Programme under Grant Agreement 242095 (EviMalar), and a Medical Research Council Career Development fellowship (to R. W. M.). A. P. was funded by his faculty baseline support and Office for Competitive Research Funds from the King Abdullah University of Science and Technology.en
dc.publisherProceedings of the National Academy of Sciencesen
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3545754en
dc.subjectInvasionen
dc.subjectTransfectionen
dc.titleAdaptation of the genetically tractable malaria pathogen Plasmodium knowlesi to continuous culture in human erythrocytesen
dc.typeArticleen
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentBioscience Programen
dc.contributor.departmentComputer Science Programen
dc.contributor.departmentPathogen Genomics Laboratoryen
dc.identifier.journalProceedings of the National Academy of Sciencesen
dc.identifier.pmcidPMC3545754en
dc.contributor.institutionNatl Inst Med Res, MRC, Div Parasitol, London NW7 1AA, Englanden
dc.contributor.institutionHlth Protect Agcy, Natl Inst Biol Stand & Controls, Div Retrovirol, Potters Bar EN6 3QG, Herts, Englanden
dc.contributor.institutionGuys Hosp, Kings Hosp, Sch Med Guys, Peter Gorer Dept Immunobiol,Kings Coll London, London SE1 9RT, Englanden
dc.contributor.institutionGuys Hosp, St Thomas Hosp, London SE1 9RT, Englanden
kaust.authorRangkuti, Farania Gama Ardhinaen
kaust.authorPain, Arnaben
kaust.authorHo, YungShwenen

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