Development of 35 novel microsatellite markers for the two-band anemonefish Amphiprion bicinctus

Handle URI:
http://hdl.handle.net/10754/562460
Title:
Development of 35 novel microsatellite markers for the two-band anemonefish Amphiprion bicinctus
Authors:
Nanninga, Gerrit B. ( 0000-0002-0134-1689 ) ; Mughal, Mehreen; Saenz Agudelo, Pablo; Bayer, Till; Berumen, Michael L. ( 0000-0003-2463-2742 )
Abstract:
To investigate population genetic patterns of the anemonefish Amphiprion bicinctus in the Red Sea, we isolated and characterized 35 microsatellite loci using 454-sequencing. Microsatellite sequences were identified using the Tandem Repeats Finder program. The 35 loci were tested on 80 individuals sampled from two spatially separated populations along the Saudi Arabian Red Sea coast. We found a mean of 10. 9 alleles per locus and observed levels of heterozygosity ranged from 0. 4 to 0. 98. All loci were polymorphic, none deviated significantly from Hardy-Weinberg equilibrium, no linkage disequilibrium was observed and there was no evidence for -alleles in both populations. The markers reported here constitute the first specific set for this species, and they are expected to contribute to future studies of connectivity in the Red Sea region. © 2012 Springer Science+Business Media Dordrecht.
KAUST Department:
Red Sea Research Center (RSRC); Biological and Environmental Sciences and Engineering (BESE) Division; Marine Science Program; Reef Ecology Lab
Publisher:
Springer Nature
Journal:
Conservation Genetics Resources
Issue Date:
18-Dec-2012
DOI:
10.1007/s12686-012-9841-0
Type:
Article
ISSN:
18777252
Appears in Collections:
Articles; Red Sea Research Center (RSRC); Marine Science Program; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorNanninga, Gerrit B.en
dc.contributor.authorMughal, Mehreenen
dc.contributor.authorSaenz Agudelo, Pabloen
dc.contributor.authorBayer, Tillen
dc.contributor.authorBerumen, Michael L.en
dc.date.accessioned2015-08-03T10:39:00Zen
dc.date.available2015-08-03T10:39:00Zen
dc.date.issued2012-12-18en
dc.identifier.issn18777252en
dc.identifier.doi10.1007/s12686-012-9841-0en
dc.identifier.urihttp://hdl.handle.net/10754/562460en
dc.description.abstractTo investigate population genetic patterns of the anemonefish Amphiprion bicinctus in the Red Sea, we isolated and characterized 35 microsatellite loci using 454-sequencing. Microsatellite sequences were identified using the Tandem Repeats Finder program. The 35 loci were tested on 80 individuals sampled from two spatially separated populations along the Saudi Arabian Red Sea coast. We found a mean of 10. 9 alleles per locus and observed levels of heterozygosity ranged from 0. 4 to 0. 98. All loci were polymorphic, none deviated significantly from Hardy-Weinberg equilibrium, no linkage disequilibrium was observed and there was no evidence for -alleles in both populations. The markers reported here constitute the first specific set for this species, and they are expected to contribute to future studies of connectivity in the Red Sea region. © 2012 Springer Science+Business Media Dordrecht.en
dc.publisherSpringer Natureen
dc.subjectAmphiprioninaeen
dc.subjectMicrosatellitesen
dc.subjectPopulation geneticsen
dc.subjectTwoband clownfishen
dc.titleDevelopment of 35 novel microsatellite markers for the two-band anemonefish Amphiprion bicinctusen
dc.typeArticleen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentMarine Science Programen
dc.contributor.departmentReef Ecology Laben
dc.identifier.journalConservation Genetics Resourcesen
dc.contributor.institutionBiology Department, Woods Hole Oceanographic Institution, Woods Hole, MA, 02543, United Statesen
kaust.authorNanninga, Gerrit B.en
kaust.authorSaenz Agudelo, Pabloen
kaust.authorBayer, Tillen
kaust.authorBerumen, Michael L.en
kaust.authorMughal, Mehreenen
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