Circular dichroism measured on single chlorosomal light-harvesting complexes of green photosynthetic bacteria

Handle URI:
http://hdl.handle.net/10754/562454
Title:
Circular dichroism measured on single chlorosomal light-harvesting complexes of green photosynthetic bacteria
Authors:
Furumaki, Shu; Yabiku, Yu; Habuchi, Satoshi ( 0000-0002-6663-2807 ) ; Tsukatani, Yusuke; Bryant, Donald A.; Vácha, Martin
Abstract:
We report results on circular dichroism (CD) measured on single immobilized chlorosomes of a triple mutant of green sulfur bacterium Chlorobaculum tepidum. The CD signal is measured by monitoring chlorosomal bacteriochlorphyll c fluorescence excited by alternate left and right circularly polarized laser light with a fixed wavelength of 733 nm. The excitation wavelength is close to a maximum of the negative CD signal of a bulk solution of the same chlorosomes. The average CD dissymmetry parameter obtained from an ensemble of individual chlorosomes was gs = -0.025, with an intrinsic standard deviation (due to variations between individual chlorosomes) of 0.006. The dissymmetry value is about 2.5 times larger than that obtained at the same wavelength in the bulk solution. The difference can be satisfactorily explained by taking into account the orientation factor in the single-chlorosome experiments. The observed distribution of the dissymmetry parameter reflects the well-ordered nature of the mutant chlorosomes. © 2012 American Chemical Society.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program; Single-Molecule Spectroscopy and Microscopy Research Group
Publisher:
American Chemical Society (ACS)
Journal:
The Journal of Physical Chemistry Letters
Issue Date:
6-Dec-2012
DOI:
10.1021/jz301671p
Type:
Article
ISSN:
19487185
Sponsors:
The work was supported by a Grant-in-Aid for Scientific Research No. 23651107 of the Japan Society for the Promotion of Science (M.V.).
Appears in Collections:
Articles; Bioscience Program; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorFurumaki, Shuen
dc.contributor.authorYabiku, Yuen
dc.contributor.authorHabuchi, Satoshien
dc.contributor.authorTsukatani, Yusukeen
dc.contributor.authorBryant, Donald A.en
dc.contributor.authorVácha, Martinen
dc.date.accessioned2015-08-03T10:38:46Zen
dc.date.available2015-08-03T10:38:46Zen
dc.date.issued2012-12-06en
dc.identifier.issn19487185en
dc.identifier.doi10.1021/jz301671pen
dc.identifier.urihttp://hdl.handle.net/10754/562454en
dc.description.abstractWe report results on circular dichroism (CD) measured on single immobilized chlorosomes of a triple mutant of green sulfur bacterium Chlorobaculum tepidum. The CD signal is measured by monitoring chlorosomal bacteriochlorphyll c fluorescence excited by alternate left and right circularly polarized laser light with a fixed wavelength of 733 nm. The excitation wavelength is close to a maximum of the negative CD signal of a bulk solution of the same chlorosomes. The average CD dissymmetry parameter obtained from an ensemble of individual chlorosomes was gs = -0.025, with an intrinsic standard deviation (due to variations between individual chlorosomes) of 0.006. The dissymmetry value is about 2.5 times larger than that obtained at the same wavelength in the bulk solution. The difference can be satisfactorily explained by taking into account the orientation factor in the single-chlorosome experiments. The observed distribution of the dissymmetry parameter reflects the well-ordered nature of the mutant chlorosomes. © 2012 American Chemical Society.en
dc.description.sponsorshipThe work was supported by a Grant-in-Aid for Scientific Research No. 23651107 of the Japan Society for the Promotion of Science (M.V.).en
dc.publisherAmerican Chemical Society (ACS)en
dc.titleCircular dichroism measured on single chlorosomal light-harvesting complexes of green photosynthetic bacteriaen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentBioscience Programen
dc.contributor.departmentSingle-Molecule Spectroscopy and Microscopy Research Groupen
dc.identifier.journalThe Journal of Physical Chemistry Lettersen
dc.contributor.institutionDepartment of Organic and Polymeric Materials, Tokyo Institute of Technology, Ookayama 2-12-1-S8, Meguro-ku, Tokyo 152-8552, Japanen
dc.contributor.institutionDepartment of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16802, United Statesen
dc.contributor.institutionDepartment of Chemistry and Biochemistry, Montana State University, Bozeman, MN 59717, United Statesen
dc.contributor.institutionGraduate School of Life Sciences, Ritsumeikan University, Shiga 525-8577, Japanen
dc.contributor.institutionPRESTO, Japan Science and Technology Agency, Saitama 332-0012, Japanen
kaust.authorHabuchi, Satoshien
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