Leaf curl diseases of two solanaceous species in Southwest Arabia are caused by a monopartite begomovirus evolutionarily most closely related to a species from the Nile Basin and unique suite of betasatellites

Handle URI:
http://hdl.handle.net/10754/562344
Title:
Leaf curl diseases of two solanaceous species in Southwest Arabia are caused by a monopartite begomovirus evolutionarily most closely related to a species from the Nile Basin and unique suite of betasatellites
Authors:
Idris, Ali; Abdullah, N.; Brown, Judith K.
Abstract:
The complete genome of 2780 bases was amplified using rolling circle amplification, and cloned, and sequenced for two distinct strains of the monopartite begomovirus Tomato leaf curl Sudan virus (ToLCSDV). The two strains shared 86-91% identity with the previously described ToLCSDV from the Nile Basin, and 90-91% identity with one another. One strain was cloned from symptomatic tomato plants from Tihamah (ToLCSDV-YE[YE:Tih:05]) while the other was cloned from symptomatic tobacco plants collected from Wadi Hadramaut (ToLCSDV-YE[YE:Had:89]). A distinct full-length betasatellite molecule (1352 bases) was cloned from the respective field-infected tomato and tobacco plants. Agro-inoculation of tomato and Nicotiana benthamiana plants with cloned partial tandem repeats of ToLCSDV-YE[YE:Tih11:05]) and the associated betasatellite, Tomato leaf curl Yemen betasatellite (ToLCYEB-[Tih:tom:137:05]), resulted in the reproduction of leaf curl disease symptoms in test plants like those observed in the field-infected plants. The betasatellite contributed to symptom severity in N. benthamiana test plants when it was co-inoculated with ToLCSDV-YE, compared to the milder symptoms that were observed in tobacco plants infected with the helper virus alone. © 2012 Elsevier B.V.
KAUST Department:
Center for Desert Agriculture
Publisher:
Elsevier BV
Journal:
Virus Research
Issue Date:
Oct-2012
DOI:
10.1016/j.virusres.2012.07.014
PubMed ID:
22841489
Type:
Article
ISSN:
01681702
Sponsors:
This research was supported in part by a SABIC Fellowship to the first author, and a Fulbright scholarship to the second author.
Appears in Collections:
Articles; Center for Desert Agriculture

Full metadata record

DC FieldValue Language
dc.contributor.authorIdris, Alien
dc.contributor.authorAbdullah, N.en
dc.contributor.authorBrown, Judith K.en
dc.date.accessioned2015-08-03T10:01:44Zen
dc.date.available2015-08-03T10:01:44Zen
dc.date.issued2012-10en
dc.identifier.issn01681702en
dc.identifier.pmid22841489en
dc.identifier.doi10.1016/j.virusres.2012.07.014en
dc.identifier.urihttp://hdl.handle.net/10754/562344en
dc.description.abstractThe complete genome of 2780 bases was amplified using rolling circle amplification, and cloned, and sequenced for two distinct strains of the monopartite begomovirus Tomato leaf curl Sudan virus (ToLCSDV). The two strains shared 86-91% identity with the previously described ToLCSDV from the Nile Basin, and 90-91% identity with one another. One strain was cloned from symptomatic tomato plants from Tihamah (ToLCSDV-YE[YE:Tih:05]) while the other was cloned from symptomatic tobacco plants collected from Wadi Hadramaut (ToLCSDV-YE[YE:Had:89]). A distinct full-length betasatellite molecule (1352 bases) was cloned from the respective field-infected tomato and tobacco plants. Agro-inoculation of tomato and Nicotiana benthamiana plants with cloned partial tandem repeats of ToLCSDV-YE[YE:Tih11:05]) and the associated betasatellite, Tomato leaf curl Yemen betasatellite (ToLCYEB-[Tih:tom:137:05]), resulted in the reproduction of leaf curl disease symptoms in test plants like those observed in the field-infected plants. The betasatellite contributed to symptom severity in N. benthamiana test plants when it was co-inoculated with ToLCSDV-YE, compared to the milder symptoms that were observed in tobacco plants infected with the helper virus alone. © 2012 Elsevier B.V.en
dc.description.sponsorshipThis research was supported in part by a SABIC Fellowship to the first author, and a Fulbright scholarship to the second author.en
dc.publisherElsevier BVen
dc.subjectGeminiviridaeen
dc.subjectKoch's postulatesen
dc.subjectTobaccoen
dc.subjectTomato virusen
dc.titleLeaf curl diseases of two solanaceous species in Southwest Arabia are caused by a monopartite begomovirus evolutionarily most closely related to a species from the Nile Basin and unique suite of betasatellitesen
dc.typeArticleen
dc.contributor.departmentCenter for Desert Agricultureen
dc.identifier.journalVirus Researchen
dc.contributor.institutionUniv Arizona, Sch Plant Sci, Tucson, AZ 85721 USAen
dc.contributor.institutionSanaa Univ, Dept Plant Protect, Sanaa, Yemenen
kaust.authorIdris, Alien
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