Neocellularization and neovascularization of nanosized bioactive glass-coated decellularized trabecular bone scaffolds

Handle URI:
http://hdl.handle.net/10754/562325
Title:
Neocellularization and neovascularization of nanosized bioactive glass-coated decellularized trabecular bone scaffolds
Authors:
Gerhardt, Lutz Christian; Widdows, Kate L.; Erol, Melek M.; Nandakumar, Anandkumar; Roqan, Iman S. ( 0000-0001-7442-4330 ) ; Ansari, Tahera I.; Boccaccini, Aldo R.
Abstract:
In this study, the in vivo recellularization and neovascularization of nanosized bioactive glass (n-BG)-coated decellu-larized trabecular bone scaffolds were studied in a rat model and quantified using stereological analyses. Based on the highest amount of vascular endothelial growth factor (VEGF) secreted by human fibroblasts grown on n-BG coatings (0-1.245 mg/cm 2), decellularized trabecular bone samples (porosity: 43-81%) were coated with n-BG particles. Grown on n-BG particles at a coating density of 0.263 mg/cm2, human fibroblasts produced 4.3 times more VEGF than on uncoated controls. After 8 weeks of implantation in Sprague-Dawley rats, both uncoated and n-BG-coated samples were well infiltrated with newly formed tissue (47-48%) and blood vessels (3-4%). No significant differences were found in cellularization and vascularization between uncoated bone scaffolds and n-BG-coated scaffolds. This finding indicates that the decellularized bone itself may exhibit growth-promoting properties induced by the highly interconnected pore microarchitecture and/or proteins left behind on decellularized scaffolds. Even if we did not find proangiogenic effects in n-BG-coated bone scaffolds, a bioactive coating is considered to be beneficial to impart osteoinductive and osteoconductive properties to decellularized bone. n-BG-coated bone grafts have thus high clinical potential for the regeneration of complex tissue defects given their ability for recellularization and neovascularization. © 2012 Wiley Periodicals, Inc.
KAUST Department:
Physical Sciences and Engineering (PSE) Division; Materials Science and Engineering Program; Semiconductor and Material Spectroscopy (SMS) Laboratory
Publisher:
Wiley-Blackwell
Journal:
Journal of Biomedical Materials Research Part A
Issue Date:
11-Sep-2012
DOI:
10.1002/jbm.a.34373
PubMed ID:
22968899
Type:
Article
ISSN:
15493296
Sponsors:
Contract grant sponsors: Partial funding by King Abdullah University of Science and Technology (KAUST; 2009-2010)
Appears in Collections:
Articles; Physical Sciences and Engineering (PSE) Division; Materials Science and Engineering Program

Full metadata record

DC FieldValue Language
dc.contributor.authorGerhardt, Lutz Christianen
dc.contributor.authorWiddows, Kate L.en
dc.contributor.authorErol, Melek M.en
dc.contributor.authorNandakumar, Anandkumaren
dc.contributor.authorRoqan, Iman S.en
dc.contributor.authorAnsari, Tahera I.en
dc.contributor.authorBoccaccini, Aldo R.en
dc.date.accessioned2015-08-03T10:00:58Zen
dc.date.available2015-08-03T10:00:58Zen
dc.date.issued2012-09-11en
dc.identifier.issn15493296en
dc.identifier.pmid22968899en
dc.identifier.doi10.1002/jbm.a.34373en
dc.identifier.urihttp://hdl.handle.net/10754/562325en
dc.description.abstractIn this study, the in vivo recellularization and neovascularization of nanosized bioactive glass (n-BG)-coated decellu-larized trabecular bone scaffolds were studied in a rat model and quantified using stereological analyses. Based on the highest amount of vascular endothelial growth factor (VEGF) secreted by human fibroblasts grown on n-BG coatings (0-1.245 mg/cm 2), decellularized trabecular bone samples (porosity: 43-81%) were coated with n-BG particles. Grown on n-BG particles at a coating density of 0.263 mg/cm2, human fibroblasts produced 4.3 times more VEGF than on uncoated controls. After 8 weeks of implantation in Sprague-Dawley rats, both uncoated and n-BG-coated samples were well infiltrated with newly formed tissue (47-48%) and blood vessels (3-4%). No significant differences were found in cellularization and vascularization between uncoated bone scaffolds and n-BG-coated scaffolds. This finding indicates that the decellularized bone itself may exhibit growth-promoting properties induced by the highly interconnected pore microarchitecture and/or proteins left behind on decellularized scaffolds. Even if we did not find proangiogenic effects in n-BG-coated bone scaffolds, a bioactive coating is considered to be beneficial to impart osteoinductive and osteoconductive properties to decellularized bone. n-BG-coated bone grafts have thus high clinical potential for the regeneration of complex tissue defects given their ability for recellularization and neovascularization. © 2012 Wiley Periodicals, Inc.en
dc.description.sponsorshipContract grant sponsors: Partial funding by King Abdullah University of Science and Technology (KAUST; 2009-2010)en
dc.publisherWiley-Blackwellen
dc.subjectAngiogenesisen
dc.subjectBioactive glassen
dc.subjectCoatingen
dc.subjectDecellularized boneen
dc.subjectScaffolden
dc.subjectStereologyen
dc.subjectVascular endothelial growth factoren
dc.subjectVascularizationen
dc.titleNeocellularization and neovascularization of nanosized bioactive glass-coated decellularized trabecular bone scaffoldsen
dc.typeArticleen
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.contributor.departmentMaterials Science and Engineering Programen
dc.contributor.departmentSemiconductor and Material Spectroscopy (SMS) Laboratoryen
dc.identifier.journalJournal of Biomedical Materials Research Part Aen
dc.contributor.institutionDepartment of Materials, Imperial College London, Prince Consort Road, London SW7 2BP, United Kingdomen
dc.contributor.institutionDepartment of Surgical Research, NPIMR, Watford Road, Harrow HA1 3UJ, United Kingdomen
dc.contributor.institutionDepartment of Chemical Engineering, Istanbul Technical University, Maslak, Istanbul 34469, Turkeyen
dc.contributor.institutionXeltis B.V., De Lismortel 31, 5600 AB Eindhoven, Netherlandsen
dc.contributor.institutionInstitute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Cauerstrasse 6, Erlangen 91058, Germanyen
kaust.authorRoqan, Iman S.en

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