IAA-Ala Resistant3, an evolutionarily conserved target of miR167, mediates Arabidopsis root architecture changes during high osmotic stress

Handle URI:
http://hdl.handle.net/10754/562315
Title:
IAA-Ala Resistant3, an evolutionarily conserved target of miR167, mediates Arabidopsis root architecture changes during high osmotic stress
Authors:
Kinoshita, Natsuko; Wang, Huan; Kasahara, Hiroyuki; Liu, Jun; MacPherson, Cameron R.; Machida, Yasunori; Kamiya, Yuji; Hannah, Matthew A.; Chuaa, Nam Hai
Abstract:
The functions of microRNAs and their target mRNAs in Arabidopsis thaliana development have been widely documented; however, roles of stress-responsive microRNAs and their targets are not as well understood. Using small RNA deep sequencing and ATH1 microarrays to profile mRNAs, we identified IAA-Ala Resistant3 (IAR3) as a new target of miR167a. As expected, IAR3 mRNA was cleaved at the miR167a complementary site and under high osmotic stress miR167a levels decreased, whereas IAR3 mRNA levels increased. IAR3 hydrolyzes an inactive form of auxin (indole-3-acetic acid [IAA]-alanine) and releases bioactive auxin (IAA), a central phytohormone for root development. In contrast with the wild type, iar3 mutants accumulated reduced IAA levels and did not display high osmotic stress-induced root architecture changes. Transgenic plants expressing a cleavage-resistant form of IAR3 mRNA accumulated high levels of IAR3 mRNAs and showed increased lateral root development compared with transgenic plants expressing wild-type IAR3. Expression of an inducible noncoding RNA to sequester miR167a by target mimicry led to an increase in IAR3 mRNA levels, further confirming the inverse relationship between the two partners. Sequence comparison revealed the miR167 target site on IAR3 mRNA is conserved in evolutionarily distant plant species. Finally, we showed that IAR3 is required for drought tolerance. © 2012 American Society of Plant Biologists. All rights reserved.
KAUST Department:
Computational Bioscience Research Center (CBRC); Bioscience Program
Publisher:
American Society of Plant Biologists (ASPB)
Journal:
The Plant Cell
Issue Date:
1-Sep-2012
DOI:
10.1105/tpc.112.097006
PubMed ID:
22960911
PubMed Central ID:
PMC3480289
Type:
Article
ISSN:
10404651
Sponsors:
We thank Jason Reed for the generous gifts of arf6-2 and arf8-3 single mutant and arf6-2 arf8-3 double mutant seeds, our laboratory members for discussions, Nagarajan Chandramouli of Rockefeller Proteomics Resource Center for sample preparation relating to free IAA measurement, and Scott Dewell of the Genomics Resource Center for deep sequencing. N.K. was supported by postdoctoral fellowships from the Uehara Memorial Foundation, Swiss National Science Foundation (for Prospective Researchers), and the Japan Society for the Promotion of Science. This work was supported in part by Bayer Crop Science and in part by the Cooperative Research Program for Agricultural Science and Technology Development (PJ906910), Rural Development Administration, Republic of Korea.
Additional Links:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3480289
Appears in Collections:
Articles; Bioscience Program; Computational Bioscience Research Center (CBRC)

Full metadata record

DC FieldValue Language
dc.contributor.authorKinoshita, Natsukoen
dc.contributor.authorWang, Huanen
dc.contributor.authorKasahara, Hiroyukien
dc.contributor.authorLiu, Junen
dc.contributor.authorMacPherson, Cameron R.en
dc.contributor.authorMachida, Yasunorien
dc.contributor.authorKamiya, Yujien
dc.contributor.authorHannah, Matthew A.en
dc.contributor.authorChuaa, Nam Haien
dc.date.accessioned2015-08-03T10:00:32Zen
dc.date.available2015-08-03T10:00:32Zen
dc.date.issued2012-09-01en
dc.identifier.issn10404651en
dc.identifier.pmid22960911en
dc.identifier.doi10.1105/tpc.112.097006en
dc.identifier.urihttp://hdl.handle.net/10754/562315en
dc.description.abstractThe functions of microRNAs and their target mRNAs in Arabidopsis thaliana development have been widely documented; however, roles of stress-responsive microRNAs and their targets are not as well understood. Using small RNA deep sequencing and ATH1 microarrays to profile mRNAs, we identified IAA-Ala Resistant3 (IAR3) as a new target of miR167a. As expected, IAR3 mRNA was cleaved at the miR167a complementary site and under high osmotic stress miR167a levels decreased, whereas IAR3 mRNA levels increased. IAR3 hydrolyzes an inactive form of auxin (indole-3-acetic acid [IAA]-alanine) and releases bioactive auxin (IAA), a central phytohormone for root development. In contrast with the wild type, iar3 mutants accumulated reduced IAA levels and did not display high osmotic stress-induced root architecture changes. Transgenic plants expressing a cleavage-resistant form of IAR3 mRNA accumulated high levels of IAR3 mRNAs and showed increased lateral root development compared with transgenic plants expressing wild-type IAR3. Expression of an inducible noncoding RNA to sequester miR167a by target mimicry led to an increase in IAR3 mRNA levels, further confirming the inverse relationship between the two partners. Sequence comparison revealed the miR167 target site on IAR3 mRNA is conserved in evolutionarily distant plant species. Finally, we showed that IAR3 is required for drought tolerance. © 2012 American Society of Plant Biologists. All rights reserved.en
dc.description.sponsorshipWe thank Jason Reed for the generous gifts of arf6-2 and arf8-3 single mutant and arf6-2 arf8-3 double mutant seeds, our laboratory members for discussions, Nagarajan Chandramouli of Rockefeller Proteomics Resource Center for sample preparation relating to free IAA measurement, and Scott Dewell of the Genomics Resource Center for deep sequencing. N.K. was supported by postdoctoral fellowships from the Uehara Memorial Foundation, Swiss National Science Foundation (for Prospective Researchers), and the Japan Society for the Promotion of Science. This work was supported in part by Bayer Crop Science and in part by the Cooperative Research Program for Agricultural Science and Technology Development (PJ906910), Rural Development Administration, Republic of Korea.en
dc.publisherAmerican Society of Plant Biologists (ASPB)en
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3480289en
dc.titleIAA-Ala Resistant3, an evolutionarily conserved target of miR167, mediates Arabidopsis root architecture changes during high osmotic stressen
dc.typeArticleen
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.contributor.departmentBioscience Programen
dc.identifier.journalThe Plant Cellen
dc.identifier.pmcidPMC3480289en
dc.contributor.institutionLaboratory of Plant Molecular Biology, The Rockefeller University, New York, NY 10065, United Statesen
dc.contributor.institutionDivision of Biological Science, Graduate School of Science, Nagoya University, Chikusa-ku, Nagoya 464-8602, Japanen
dc.contributor.institutionPlant Science Center, RIKEN, Yokohama, Kanagawa 230-0045, Japanen
dc.contributor.institutionBayer BioScience, Ghent 9052, Belgiumen
kaust.authorMacPherson, Cameron R.en
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