Acclimation increases freezing stress response of Arabidopsis thaliana at proteome level

Handle URI:
http://hdl.handle.net/10754/562210
Title:
Acclimation increases freezing stress response of Arabidopsis thaliana at proteome level
Authors:
Fanucchi, Francesca; Alpi, Emanuele; Olivieri, Stefano; Cannistraci, Carlo; Bachi, Angela; Alpi, Amedeo; Alessio, Massimo
Abstract:
This study used 2DE to investigate how Arabidopsis thaliana modulates protein levels in response to freezing stress after sub-lethal exposure at - 10 °C, both in cold-acclimated and in non-acclimated plants. A map was implemented in which 62 spots, corresponding to 44 proteins, were identified. Twenty-two spots were modulated upon treatments, and the corresponding proteins proved to be related to photosynthesis, energy metabolism, and stress response. Proteins demonstrated differences between control and acclimation conditions. Most of the acclimation-responsive proteins were either not further modulated or they were down-modulated by freezing treatment, indicating that the levels reached during acclimation were sufficient to deal with freezing. Anabolic metabolism appeared to be down-regulated in favor of catabolic metabolism. Acclimated plants and plants submitted to freezing after acclimation showed greater reciprocal similarity in protein profiles than either showed when compared both to control plants and to plants frozen without acclimation. The response of non-acclimated plants was aimed at re-modulating photosynthetic apparatus activity, and at increasing the levels of proteins with antioxidant-, molecular chaperone-, or post-transcriptional regulative functions. These changes, even less effective than the acclimation strategy, might allow the injured plastids to minimize the production of non-useful metabolites and might counteract photosynthetic apparatus injuries. © 2012 Elsevier B.V. All rights reserved.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Computational Bioscience Research Center (CBRC)
Publisher:
Elsevier BV
Journal:
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics
Issue Date:
Jun-2012
DOI:
10.1016/j.bbapap.2012.03.015
PubMed ID:
22510494
Type:
Article
ISSN:
15709639
Sponsors:
Massimo Alessio is supported by MoH RF-FSR-2007-637144 and by AIRC.
Appears in Collections:
Articles; Computational Bioscience Research Center (CBRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorFanucchi, Francescaen
dc.contributor.authorAlpi, Emanueleen
dc.contributor.authorOlivieri, Stefanoen
dc.contributor.authorCannistraci, Carloen
dc.contributor.authorBachi, Angelaen
dc.contributor.authorAlpi, Amedeoen
dc.contributor.authorAlessio, Massimoen
dc.date.accessioned2015-08-03T09:47:20Zen
dc.date.available2015-08-03T09:47:20Zen
dc.date.issued2012-06en
dc.identifier.issn15709639en
dc.identifier.pmid22510494en
dc.identifier.doi10.1016/j.bbapap.2012.03.015en
dc.identifier.urihttp://hdl.handle.net/10754/562210en
dc.description.abstractThis study used 2DE to investigate how Arabidopsis thaliana modulates protein levels in response to freezing stress after sub-lethal exposure at - 10 °C, both in cold-acclimated and in non-acclimated plants. A map was implemented in which 62 spots, corresponding to 44 proteins, were identified. Twenty-two spots were modulated upon treatments, and the corresponding proteins proved to be related to photosynthesis, energy metabolism, and stress response. Proteins demonstrated differences between control and acclimation conditions. Most of the acclimation-responsive proteins were either not further modulated or they were down-modulated by freezing treatment, indicating that the levels reached during acclimation were sufficient to deal with freezing. Anabolic metabolism appeared to be down-regulated in favor of catabolic metabolism. Acclimated plants and plants submitted to freezing after acclimation showed greater reciprocal similarity in protein profiles than either showed when compared both to control plants and to plants frozen without acclimation. The response of non-acclimated plants was aimed at re-modulating photosynthetic apparatus activity, and at increasing the levels of proteins with antioxidant-, molecular chaperone-, or post-transcriptional regulative functions. These changes, even less effective than the acclimation strategy, might allow the injured plastids to minimize the production of non-useful metabolites and might counteract photosynthetic apparatus injuries. © 2012 Elsevier B.V. All rights reserved.en
dc.description.sponsorshipMassimo Alessio is supported by MoH RF-FSR-2007-637144 and by AIRC.en
dc.publisherElsevier BVen
dc.subject2D electrophoresisen
dc.subjectArabidopsis thalianaen
dc.subjectFreezing stressen
dc.subjectMass spectrometryen
dc.titleAcclimation increases freezing stress response of Arabidopsis thaliana at proteome levelen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.identifier.journalBiochimica et Biophysica Acta (BBA) - Proteins and Proteomicsen
dc.contributor.institutionDepartment of Crop Plant Biology, University of Pisa, via Mariscoglio 34, 56100 Pisa, Italyen
dc.contributor.institutionBiological Mass Spectrometry Unit, San Raffaele Scientific Institute, Milan, Italyen
dc.contributor.institutionProteome Biochemistry Unit, San Raffaele Scientific Institute, Milan, Italyen
dc.contributor.institutionDepartment of Mechanics, Politecnico di Torino, Turin, Italyen
kaust.authorCannistraci, Carloen

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