Arabidopsis ECERIFERUM9 involvement in cuticle formation and maintenance of plant water status

Handle URI:
http://hdl.handle.net/10754/562195
Title:
Arabidopsis ECERIFERUM9 involvement in cuticle formation and maintenance of plant water status
Authors:
Lu, Shiyou; Zhao, Huayan; Des Marais, David L.; Parsons, Eugene P.; Wen, Xiaoxue; Xu, Xiaojing; Bangarusamy, Dhinoth Kumar; Wang, Guangchao; Rowland, Owen; Juenger, Thomas E.; Bressan, Ray Anthony; Jenks, Matthew A.
Abstract:
Mutation of the ECERIFERUM9 (CER9) gene in Arabidopsis (Arabidopsis thaliana) causes elevated amounts of 18-carbon-length cutin monomers and a dramatic shift in the cuticular wax profile (especially on leaves) toward the very-long-chain free fatty acids tetracosanoic acid (C24) and hexacosanoic acid (C26). Relative to the wild type, cer9 mutants exhibit elevated cuticle membrane thickness over epidermal cells and cuticular ledges with increased occlusion of the stomatal pore. The cuticular phenotypes of cer9 are associated with delayed onset of wilting in plants experiencing water deficit, lower transpiration rates, and improved water use efficiency measured as carbon isotope discrimination. The CER9 protein thus encodes a novel determinant of plant drought tolerance-associated traits, one whose deficiency elevates cutin synthesis, redistributes wax composition, and suppresses transpiration. Map-based cloning identified CER9, and sequence analysis predicted that it encodes an E3 ubiquitin ligase homologous to yeast Doa10 (previously shown to target endoplasmic reticulum proteins for proteasomal degradation). To further elucidate CER9 function, the impact of CER9 deficiency on interactions with other genes was examined using double mutant and transcriptome analyses. For both wax and cutin, cer9 showed mostly additive effects with cer6, long-chain acyl-CoA synthetase1 (lacs1), and lacs2 and revealed its role in early steps of both wax and cutin synthetic pathways. Transcriptome analysis revealed that the cer9 mutation affected diverse cellular processes, with primary impact on genes associated with diverse stress responses. The discovery of CER9 lays new groundwork for developing novel cuticle-based strategies for improving the drought tolerance and water use efficiency of crop plants. © 2012 American Society of Plant Biologists. All Rights Reserved.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program; Core Labs
Publisher:
American Society of Plant Biologists
Journal:
Plant Physiology
Issue Date:
25-May-2012
DOI:
10.1104/pp.112.198697
PubMed ID:
22635115
PubMed Central ID:
PMC3387718
Type:
Article
ISSN:
00320889
Sponsors:
This work was supported by the Natural Sciences and Engineering Research Council of Canada (Discovery grant to O.R.), the National Science Foundation (grant no. DEB-0618347 to T.J.), and the U.S. Department of Agriculture (National Institute of Food and Agriculture Biomass Research and Development Initiative grant to M.A.J.).
Additional Links:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3387718
Appears in Collections:
Articles; Bioscience Program; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorLu, Shiyouen
dc.contributor.authorZhao, Huayanen
dc.contributor.authorDes Marais, David L.en
dc.contributor.authorParsons, Eugene P.en
dc.contributor.authorWen, Xiaoxueen
dc.contributor.authorXu, Xiaojingen
dc.contributor.authorBangarusamy, Dhinoth Kumaren
dc.contributor.authorWang, Guangchaoen
dc.contributor.authorRowland, Owenen
dc.contributor.authorJuenger, Thomas E.en
dc.contributor.authorBressan, Ray Anthonyen
dc.contributor.authorJenks, Matthew A.en
dc.date.accessioned2015-08-03T09:47:00Zen
dc.date.available2015-08-03T09:47:00Zen
dc.date.issued2012-05-25en
dc.identifier.issn00320889en
dc.identifier.pmid22635115en
dc.identifier.doi10.1104/pp.112.198697en
dc.identifier.urihttp://hdl.handle.net/10754/562195en
dc.description.abstractMutation of the ECERIFERUM9 (CER9) gene in Arabidopsis (Arabidopsis thaliana) causes elevated amounts of 18-carbon-length cutin monomers and a dramatic shift in the cuticular wax profile (especially on leaves) toward the very-long-chain free fatty acids tetracosanoic acid (C24) and hexacosanoic acid (C26). Relative to the wild type, cer9 mutants exhibit elevated cuticle membrane thickness over epidermal cells and cuticular ledges with increased occlusion of the stomatal pore. The cuticular phenotypes of cer9 are associated with delayed onset of wilting in plants experiencing water deficit, lower transpiration rates, and improved water use efficiency measured as carbon isotope discrimination. The CER9 protein thus encodes a novel determinant of plant drought tolerance-associated traits, one whose deficiency elevates cutin synthesis, redistributes wax composition, and suppresses transpiration. Map-based cloning identified CER9, and sequence analysis predicted that it encodes an E3 ubiquitin ligase homologous to yeast Doa10 (previously shown to target endoplasmic reticulum proteins for proteasomal degradation). To further elucidate CER9 function, the impact of CER9 deficiency on interactions with other genes was examined using double mutant and transcriptome analyses. For both wax and cutin, cer9 showed mostly additive effects with cer6, long-chain acyl-CoA synthetase1 (lacs1), and lacs2 and revealed its role in early steps of both wax and cutin synthetic pathways. Transcriptome analysis revealed that the cer9 mutation affected diverse cellular processes, with primary impact on genes associated with diverse stress responses. The discovery of CER9 lays new groundwork for developing novel cuticle-based strategies for improving the drought tolerance and water use efficiency of crop plants. © 2012 American Society of Plant Biologists. All Rights Reserved.en
dc.description.sponsorshipThis work was supported by the Natural Sciences and Engineering Research Council of Canada (Discovery grant to O.R.), the National Science Foundation (grant no. DEB-0618347 to T.J.), and the U.S. Department of Agriculture (National Institute of Food and Agriculture Biomass Research and Development Initiative grant to M.A.J.).en
dc.publisherAmerican Society of Plant Biologistsen
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3387718en
dc.titleArabidopsis ECERIFERUM9 involvement in cuticle formation and maintenance of plant water statusen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentBioscience Programen
dc.contributor.departmentCore Labsen
dc.identifier.journalPlant Physiologyen
dc.identifier.pmcidPMC3387718en
dc.contributor.institutionSection of Integrative Biology, University of Texas, Austin, TX 78712, United Statesen
dc.contributor.institutionDepartment of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907-2054, United Statesen
dc.contributor.institutionDepartment of Biology and Institute of Biochemistry, Carleton University, Ottawa, ON K1S 5B6, Canadaen
dc.contributor.institutionUnited States Arid Land Agricultural Research Center, United States Department of Agriculture-Agricultural Research Service, Maricopa, AZ 85138, United Statesen
kaust.authorLu, Shiyouen
kaust.authorZhao, Huayanen
kaust.authorBangarusamy, Dhinoth Kumaren
kaust.authorWang, Guangchaoen
kaust.authorBressan, Ray Anthonyen

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