Multicomponent mixed dopant optimization for rapid screening of polycyclic aromatic hydrocarbons using ultra high performance liquid chromatography coupled to atmospheric pressure photoionization high-resolution mass spectrometry

Handle URI:
http://hdl.handle.net/10754/562179
Title:
Multicomponent mixed dopant optimization for rapid screening of polycyclic aromatic hydrocarbons using ultra high performance liquid chromatography coupled to atmospheric pressure photoionization high-resolution mass spectrometry
Authors:
Sioud, Salim; Amad, Maan H.; Al-Talla, Zeyad
Abstract:
RATIONALE To enhance the ionization efficiencies in atmospheric pressure photoionization mass spectrometry a dopant with favorable ionization energy such as chlorobenzene is typically used. These dopants are typically toxic and difficult to mix with water-soluble organic solvents. In order to achieve a more efficient and less toxic dopant, a multicomponent mixed dopant was explored. METHODS A multicomponent mixed dopant for non-targeted rapid screening of polycyclic aromatic hydrocarbons (PAHs) was developed and optimized using ultra high performance liquid chromatography (UPLC) coupled to atmospheric pressure photoionization high-resolution mass spectrometry. Various single and multicomponent mixed dopants consisting of ethanol, chlorobenzene, bromobenzene, anisole and toluene were evaluated. RESULTS Fourteen out of eighteen PAHs were successfully separated and detected at low pg/μL levels within 5 min with high mass accuracy ≤4 ppm. The optimal mixed multicomponent dopant consisted of ethanol/chlorobenzene/bromobenzene/anisole (98.975:0.1:0.9:0.025, v/v %) and it improved the limit of detection (LOD) by 2- to 10-fold for the tested PAHs compared to those obtained with pure chlorobenzene. CONCLUSIONS A novel multicomponent dopant that contains 99% ethanol and 1% mixture of chlorobenzene, bromobenzene and anisole was found to be an effective dopant mixture to ionize PAHs. The developed UPLC multicomponent dopant assisted atmospheric pressure photoionization high-resolution mass spectrometry offered a rapid non targeted screening method for detecting the PAHs at low pg/;μL levels within a 5 min run time with high mass accuracy a;circ4 ppm. Copyright © 2012 John Wiley & Sons, Ltd.
KAUST Department:
Analytical Core Lab; Core Labs
Publisher:
Wiley
Journal:
Rapid Communications in Mass Spectrometry
Issue Date:
4-May-2012
DOI:
10.1002/rcm.6250
Type:
Article
ISSN:
09514198
Sponsors:
The authors gratefully acknowledge the research support from the Analytical Core Lab at the King Abdullah University of Science and Technology, Saudi Arabia.
Appears in Collections:
Articles; Analytical Core Lab

Full metadata record

DC FieldValue Language
dc.contributor.authorSioud, Salimen
dc.contributor.authorAmad, Maan H.en
dc.contributor.authorAl-Talla, Zeyaden
dc.date.accessioned2015-08-03T09:46:37Zen
dc.date.available2015-08-03T09:46:37Zen
dc.date.issued2012-05-04en
dc.identifier.issn09514198en
dc.identifier.doi10.1002/rcm.6250en
dc.identifier.urihttp://hdl.handle.net/10754/562179en
dc.description.abstractRATIONALE To enhance the ionization efficiencies in atmospheric pressure photoionization mass spectrometry a dopant with favorable ionization energy such as chlorobenzene is typically used. These dopants are typically toxic and difficult to mix with water-soluble organic solvents. In order to achieve a more efficient and less toxic dopant, a multicomponent mixed dopant was explored. METHODS A multicomponent mixed dopant for non-targeted rapid screening of polycyclic aromatic hydrocarbons (PAHs) was developed and optimized using ultra high performance liquid chromatography (UPLC) coupled to atmospheric pressure photoionization high-resolution mass spectrometry. Various single and multicomponent mixed dopants consisting of ethanol, chlorobenzene, bromobenzene, anisole and toluene were evaluated. RESULTS Fourteen out of eighteen PAHs were successfully separated and detected at low pg/μL levels within 5 min with high mass accuracy ≤4 ppm. The optimal mixed multicomponent dopant consisted of ethanol/chlorobenzene/bromobenzene/anisole (98.975:0.1:0.9:0.025, v/v %) and it improved the limit of detection (LOD) by 2- to 10-fold for the tested PAHs compared to those obtained with pure chlorobenzene. CONCLUSIONS A novel multicomponent dopant that contains 99% ethanol and 1% mixture of chlorobenzene, bromobenzene and anisole was found to be an effective dopant mixture to ionize PAHs. The developed UPLC multicomponent dopant assisted atmospheric pressure photoionization high-resolution mass spectrometry offered a rapid non targeted screening method for detecting the PAHs at low pg/;μL levels within a 5 min run time with high mass accuracy a;circ4 ppm. Copyright © 2012 John Wiley & Sons, Ltd.en
dc.description.sponsorshipThe authors gratefully acknowledge the research support from the Analytical Core Lab at the King Abdullah University of Science and Technology, Saudi Arabia.en
dc.publisherWileyen
dc.titleMulticomponent mixed dopant optimization for rapid screening of polycyclic aromatic hydrocarbons using ultra high performance liquid chromatography coupled to atmospheric pressure photoionization high-resolution mass spectrometryen
dc.typeArticleen
dc.contributor.departmentAnalytical Core Laben
dc.contributor.departmentCore Labsen
dc.identifier.journalRapid Communications in Mass Spectrometryen
kaust.authorSioud, Salimen
kaust.authorAmad, Maan H.en
kaust.authorAl-Talla, Zeyaden
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