Reliable measurement of elastic modulus of cells by nanoindentation in an atomic force microscope

Handle URI:
http://hdl.handle.net/10754/562147
Title:
Reliable measurement of elastic modulus of cells by nanoindentation in an atomic force microscope
Authors:
Zhou, Zhoulong; Ngan, Alfonso H W; Tang, Bin; Wang, Anxun
Abstract:
The elastic modulus of an oral cancer cell line UM1 is investigated by nanoindentation in an atomic force microscope with a flat-ended tip. The commonly used Hertzian method gives apparent elastic modulus which increases with the loading rate, indicating strong effects of viscoelasticity. On the contrary, a rate-jump method developed for viscoelastic materials gives elastic modulus values which are independent of the rate-jump magnitude. The results show that the rate-jump method can be used as a standard protocol for measuring elastic stiffness of living cells, since the measured values are intrinsic properties of the cells. © 2011 Elsevier Ltd.
KAUST Department:
Mechanical Engineering Program; Physical Sciences and Engineering (PSE) Division
Publisher:
Elsevier BV
Journal:
Journal of the Mechanical Behavior of Biomedical Materials
Issue Date:
Apr-2012
DOI:
10.1016/j.jmbbm.2011.11.010
PubMed ID:
22402160
Type:
Article
ISSN:
17516161
Sponsors:
The work done in this article was supported by a grant from the University Grants Committee of the Hong Kong Special Administration Region, PR China (Project No. SEG-HKU06).
Appears in Collections:
Articles; Physical Sciences and Engineering (PSE) Division; Mechanical Engineering Program

Full metadata record

DC FieldValue Language
dc.contributor.authorZhou, Zhoulongen
dc.contributor.authorNgan, Alfonso H Wen
dc.contributor.authorTang, Binen
dc.contributor.authorWang, Anxunen
dc.date.accessioned2015-08-03T09:45:54Zen
dc.date.available2015-08-03T09:45:54Zen
dc.date.issued2012-04en
dc.identifier.issn17516161en
dc.identifier.pmid22402160en
dc.identifier.doi10.1016/j.jmbbm.2011.11.010en
dc.identifier.urihttp://hdl.handle.net/10754/562147en
dc.description.abstractThe elastic modulus of an oral cancer cell line UM1 is investigated by nanoindentation in an atomic force microscope with a flat-ended tip. The commonly used Hertzian method gives apparent elastic modulus which increases with the loading rate, indicating strong effects of viscoelasticity. On the contrary, a rate-jump method developed for viscoelastic materials gives elastic modulus values which are independent of the rate-jump magnitude. The results show that the rate-jump method can be used as a standard protocol for measuring elastic stiffness of living cells, since the measured values are intrinsic properties of the cells. © 2011 Elsevier Ltd.en
dc.description.sponsorshipThe work done in this article was supported by a grant from the University Grants Committee of the Hong Kong Special Administration Region, PR China (Project No. SEG-HKU06).en
dc.publisherElsevier BVen
dc.subjectAtomic force microscopyen
dc.subjectElastic modulusen
dc.subjectNanoindentationen
dc.subjectOral cancer cellsen
dc.subjectViscoelasticityen
dc.titleReliable measurement of elastic modulus of cells by nanoindentation in an atomic force microscopeen
dc.typeArticleen
dc.contributor.departmentMechanical Engineering Programen
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.identifier.journalJournal of the Mechanical Behavior of Biomedical Materialsen
dc.contributor.institutionDepartment of Mechanical Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong, Chinaen
dc.contributor.institutionDepartment of Oral and Maxillofacial Surgery, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, Chinaen
kaust.authorTang, Binen

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