Development of an avidin sensor based on the poly(methoxy amino-β-styryl terthiophene)-coated glassy carbon electrode

Handle URI:
http://hdl.handle.net/10754/562113
Title:
Development of an avidin sensor based on the poly(methoxy amino-β-styryl terthiophene)-coated glassy carbon electrode
Authors:
Mehenni, Hakim
Abstract:
In this study, a simple and direct biosensor was proposed, which was based on biotin immobilized onto a conducting polymer-coated electrode, for the determination of avidin, a highly stable glycoprotein found in egg whites. Biotin was immobilized onto the electrode by covalent coupling to the primary amine group on poly-3′-(2-methoxy-5-amino-β-styryl)-(2,2′: 5′,2″-terthiophene) (PMAST), and the biotin-avidin interaction was monitored by square-wave voltammetry. Incubation of the PMAST/biotin-modified coated electrode with avidin in a phosphate-buffered saline solution caused a significant change to its square-wave voltammogram, which was explained by the binding of avidin by biotin, and resulted in restricted ion transfer to and from the conducting polymer. This change was then utilized to determine avidin. Importantly, we found a linear relationship for the avidin sensor in the range of 4 × 10 -14 to 3 × 10 -4 mol/L, and the detection limit was determined to be approximately 10 -14 mol/L. © 2012 Published by NRC Research Press.
KAUST Department:
Functional Nanomaterials Lab (FuNL); Materials Science and Engineering Program; KAUST Catalysis Center (KCC)
Publisher:
NRC Research Press
Journal:
Canadian Journal of Chemistry
Issue Date:
Mar-2012
DOI:
10.1139/v11-160
Type:
Article
ISSN:
00084042
Sponsors:
This work was supported by the National Institute of Scientific Research (INRS) and the Natural Sciences and Engineering Research Council of Canada (NSERC).
Appears in Collections:
Articles; Materials Science and Engineering Program; KAUST Catalysis Center (KCC)

Full metadata record

DC FieldValue Language
dc.contributor.authorMehenni, Hakimen
dc.date.accessioned2015-08-03T09:45:05Zen
dc.date.available2015-08-03T09:45:05Zen
dc.date.issued2012-03en
dc.identifier.issn00084042en
dc.identifier.doi10.1139/v11-160en
dc.identifier.urihttp://hdl.handle.net/10754/562113en
dc.description.abstractIn this study, a simple and direct biosensor was proposed, which was based on biotin immobilized onto a conducting polymer-coated electrode, for the determination of avidin, a highly stable glycoprotein found in egg whites. Biotin was immobilized onto the electrode by covalent coupling to the primary amine group on poly-3′-(2-methoxy-5-amino-β-styryl)-(2,2′: 5′,2″-terthiophene) (PMAST), and the biotin-avidin interaction was monitored by square-wave voltammetry. Incubation of the PMAST/biotin-modified coated electrode with avidin in a phosphate-buffered saline solution caused a significant change to its square-wave voltammogram, which was explained by the binding of avidin by biotin, and resulted in restricted ion transfer to and from the conducting polymer. This change was then utilized to determine avidin. Importantly, we found a linear relationship for the avidin sensor in the range of 4 × 10 -14 to 3 × 10 -4 mol/L, and the detection limit was determined to be approximately 10 -14 mol/L. © 2012 Published by NRC Research Press.en
dc.description.sponsorshipThis work was supported by the National Institute of Scientific Research (INRS) and the Natural Sciences and Engineering Research Council of Canada (NSERC).en
dc.publisherNRC Research Pressen
dc.subjectAvidinen
dc.subjectBiological functionalizationen
dc.subjectBiosensoren
dc.subjectCyclic voltammetryen
dc.subjectElectrode surface modificationen
dc.subjectElectronic conducting polymeren
dc.subjectSquare-wave voltammetryen
dc.titleDevelopment of an avidin sensor based on the poly(methoxy amino-β-styryl terthiophene)-coated glassy carbon electrodeen
dc.typeArticleen
dc.contributor.departmentFunctional Nanomaterials Lab (FuNL)en
dc.contributor.departmentMaterials Science and Engineering Programen
dc.contributor.departmentKAUST Catalysis Center (KCC)en
dc.identifier.journalCanadian Journal of Chemistryen
dc.contributor.institutionLaboratoires de Recherche sur les Matériaux Organiques Avancés, INRS-Energie et Matériaux, 1650 Bd Lionel Boulet, Varennes, QC J3X 1S2, Canadaen
kaust.authorMehenni, Hakimen
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