Polyphosphonium polymers for siRNA delivery: An efficient and nontoxic alternative to polyammonium carriers

Handle URI:
http://hdl.handle.net/10754/562078
Title:
Polyphosphonium polymers for siRNA delivery: An efficient and nontoxic alternative to polyammonium carriers
Authors:
Ornelas-Megiatto, Cátia; Wich, Peter R.; Frechet, Jean ( 0000-0001-6419-0163 )
Abstract:
A water-soluble polyphosphonium polymer was synthesized and directly compared with its ammonium analog in terms of siRNA delivery. The triethylphosphonium polymer shows transfection efficiency up to 65% with 100% cell viability, whereas the best result obtained for the ammonium analog reaches only 25% transfection with 85% cell viability. Moreover, the nature of the alkyl substituents on the phosphonium cations is shown to have an important influence on the transfection efficiency and toxicity of the polyplexes. The present results show that the use of positively charged phosphonium groups is a worthy choice to achieve a good balance between toxicity and transfection efficiency in gene delivery systems. © 2012 American Chemical Society.
KAUST Department:
Chemical Science Program; Physical Sciences and Engineering (PSE) Division
Publisher:
American Chemical Society
Journal:
Journal of the American Chemical Society
Issue Date:
Feb-2012
DOI:
10.1021/ja207366k
PubMed ID:
22239619
Type:
Article
ISSN:
00027863
Sponsors:
This project has been funded in part with Federal funds from the National Heart, Lung, and Blood Institute, National Institutes of Health, Department of Health and Human Services, under Contract No. HHSN268201000043C, and in part through the Frechet "various donors" fund for the support of research in new materials. We thank Ann Fischer and Michelle Yasukawa for help with cell culture and Dr. Peter Friebe and Dr. Eva Harris for assistance with and the use of their luminescence plate reader. P.R.W. gratefully acknowledges the Alexander von Humboldt Foundation (AvH) for partial funding.
Appears in Collections:
Articles; Physical Sciences and Engineering (PSE) Division; Chemical Science Program

Full metadata record

DC FieldValue Language
dc.contributor.authorOrnelas-Megiatto, Cátiaen
dc.contributor.authorWich, Peter R.en
dc.contributor.authorFrechet, Jeanen
dc.date.accessioned2015-08-03T09:44:18Zen
dc.date.available2015-08-03T09:44:18Zen
dc.date.issued2012-02en
dc.identifier.issn00027863en
dc.identifier.pmid22239619en
dc.identifier.doi10.1021/ja207366ken
dc.identifier.urihttp://hdl.handle.net/10754/562078en
dc.description.abstractA water-soluble polyphosphonium polymer was synthesized and directly compared with its ammonium analog in terms of siRNA delivery. The triethylphosphonium polymer shows transfection efficiency up to 65% with 100% cell viability, whereas the best result obtained for the ammonium analog reaches only 25% transfection with 85% cell viability. Moreover, the nature of the alkyl substituents on the phosphonium cations is shown to have an important influence on the transfection efficiency and toxicity of the polyplexes. The present results show that the use of positively charged phosphonium groups is a worthy choice to achieve a good balance between toxicity and transfection efficiency in gene delivery systems. © 2012 American Chemical Society.en
dc.description.sponsorshipThis project has been funded in part with Federal funds from the National Heart, Lung, and Blood Institute, National Institutes of Health, Department of Health and Human Services, under Contract No. HHSN268201000043C, and in part through the Frechet "various donors" fund for the support of research in new materials. We thank Ann Fischer and Michelle Yasukawa for help with cell culture and Dr. Peter Friebe and Dr. Eva Harris for assistance with and the use of their luminescence plate reader. P.R.W. gratefully acknowledges the Alexander von Humboldt Foundation (AvH) for partial funding.en
dc.publisherAmerican Chemical Societyen
dc.titlePolyphosphonium polymers for siRNA delivery: An efficient and nontoxic alternative to polyammonium carriersen
dc.typeArticleen
dc.contributor.departmentChemical Science Programen
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.identifier.journalJournal of the American Chemical Societyen
dc.contributor.institutionCollege of Chemistry, University of California, Berkeley, CA 94720-1460, United Statesen
kaust.authorFrechet, Jeanen
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