Insights into fruit function from the proteome of the hypanthium

Handle URI:
http://hdl.handle.net/10754/562044
Title:
Insights into fruit function from the proteome of the hypanthium
Authors:
Marondedze, Claudius ( 0000-0002-2113-904X ) ; Thomas, Ludivine
Abstract:
Apple (Malus× domestica Borkh.) was used as a model to studying essential biological processes occurring in mature fruit hypanthium, commonly referred to as the fruit flesh or pulp, a highly active tissue where numerous metabolic processes such as carbohydrate metabolism and signal transduction occur. To understand the complex biological processes occurring in the hypanthium, a proteomics approach was used to analyze the proteome from freshly harvested ripe apple fruits. A total of 290 well-resolved spots were detected using two-dimensional gel electrophoresis (2-DE). Out of these, 216 proteins were identified representing 116 non-redundant proteins using matrix-assisted laser-desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) and either the MASCOT or ProteinProspector engine for peptide mass fingerprinting (PMF) database searching. Identified proteins were classified into 13 major functional categories. Among these, the energy metabolism class was the most represented and included 50% of proteins homologous to Arabidopsis proteins that are involved in the response to biotic and abiotic stresses, suggesting a dual role for these proteins in addition to energy metabolism. We also identified dynein heavy chain in the hypanthium although this protein has been proposed as absent from angiosperms and thus suggest that the lack of dyneins in higher plants studied to date may not be a general characteristic to angiosperm genomic organisation. We therefore conclude that the detection and elucidation of the apple hypanthium proteome is an indispensable step towards the comprehension of fruit metabolism, the integration of genomic, proteomic and metabolomic data to agronomic trait information and thus fruit quality improvements. © 2011 Elsevier GmbH.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program; Core Labs
Publisher:
Elsevier BV
Journal:
Journal of Plant Physiology
Issue Date:
Jan-2012
DOI:
10.1016/j.jplph.2011.10.001
PubMed ID:
22050892
Type:
Article
ISSN:
01761617
Sponsors:
This work was supported by grants from the Agricultural Research Council and Technology for Human Resources for Industry Programme, South Africa. The authors wish to thank Prof. Christoph Gehring for critically revising the manuscript and the Department of Biotechnology, University of the Western Cape, South Africa for assistance with proteomics equipment used in the study.
Appears in Collections:
Articles; Bioscience Program; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorMarondedze, Claudiusen
dc.contributor.authorThomas, Ludivineen
dc.date.accessioned2015-08-03T09:43:29Zen
dc.date.available2015-08-03T09:43:29Zen
dc.date.issued2012-01en
dc.identifier.issn01761617en
dc.identifier.pmid22050892en
dc.identifier.doi10.1016/j.jplph.2011.10.001en
dc.identifier.urihttp://hdl.handle.net/10754/562044en
dc.description.abstractApple (Malus× domestica Borkh.) was used as a model to studying essential biological processes occurring in mature fruit hypanthium, commonly referred to as the fruit flesh or pulp, a highly active tissue where numerous metabolic processes such as carbohydrate metabolism and signal transduction occur. To understand the complex biological processes occurring in the hypanthium, a proteomics approach was used to analyze the proteome from freshly harvested ripe apple fruits. A total of 290 well-resolved spots were detected using two-dimensional gel electrophoresis (2-DE). Out of these, 216 proteins were identified representing 116 non-redundant proteins using matrix-assisted laser-desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) and either the MASCOT or ProteinProspector engine for peptide mass fingerprinting (PMF) database searching. Identified proteins were classified into 13 major functional categories. Among these, the energy metabolism class was the most represented and included 50% of proteins homologous to Arabidopsis proteins that are involved in the response to biotic and abiotic stresses, suggesting a dual role for these proteins in addition to energy metabolism. We also identified dynein heavy chain in the hypanthium although this protein has been proposed as absent from angiosperms and thus suggest that the lack of dyneins in higher plants studied to date may not be a general characteristic to angiosperm genomic organisation. We therefore conclude that the detection and elucidation of the apple hypanthium proteome is an indispensable step towards the comprehension of fruit metabolism, the integration of genomic, proteomic and metabolomic data to agronomic trait information and thus fruit quality improvements. © 2011 Elsevier GmbH.en
dc.description.sponsorshipThis work was supported by grants from the Agricultural Research Council and Technology for Human Resources for Industry Programme, South Africa. The authors wish to thank Prof. Christoph Gehring for critically revising the manuscript and the Department of Biotechnology, University of the Western Cape, South Africa for assistance with proteomics equipment used in the study.en
dc.publisherElsevier BVen
dc.subject2-DEen
dc.subjectDynein heavy chainen
dc.subjectEnergy metabolismen
dc.subjectFruit hypanthiumen
dc.subjectProteomicsen
dc.titleInsights into fruit function from the proteome of the hypanthiumen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentBioscience Programen
dc.contributor.departmentCore Labsen
dc.identifier.journalJournal of Plant Physiologyen
dc.contributor.institutionDepartment of Biotechnology, University of the Western Cape, Private Bag X17, Bellville 7535, South Africaen
kaust.authorMarondedze, Claudiusen
kaust.authorThomas, Ludivineen

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